| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
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| Outline of Final Research Achievements |
Sepsis causes fibroblast proliferation in various organs following systemic inflammation due to infection. For this process, we studied growth control of fibroblasts in mouse sepsis model and human fibroblast cell culture system. Male BALB-C mice of 8 to 12 weeks as a sepsis animal model were used to evaluate pathology by cecal ligation and puncture (CLP). Analysis of the distribution of fibroblasts using S100 calcium-binding protein, etc. showed an increase in fibroblasts in the lungs and the atrial fascia interface in the time course after CLP. In the study of cell culture of human fibroblast IMR-90, it was possible to evaluate that the β receptor activation by catecholamine and thrombin activity in systemic inflammation are strongly involved in fibroblast proliferation. It was revealed that fibroblast proliferation and organization was observed in association with inflammation of sepsis in the lung and heart.
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