Gene therapy and application of iPS cells to major organ regeneration in septic multiple organ failure
Project/Area Number |
25293365
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Emergency medicine
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Research Institution | Nagoya University |
Principal Investigator |
Matsuda Naoyuki 名古屋大学, 医学(系)研究科(研究院), 教授 (50332466)
|
Project Period (FY) |
2013-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
|
Keywords | 敗血症 / 多臓器不全 / 線維芽細胞 / 転写因子 / 肺線維症 / Oct3/4 / Sox2 / Klf4 / c-Myc / 線維症 / 遺伝子治療 / 播種性血管内凝固症候群 / 再生治療 / iPS細胞 / 肺線維化 |
Outline of Final Research Achievements |
Sepsis causes fibroblast proliferation in various organs following systemic inflammation due to infection. For this process, we studied growth control of fibroblasts in mouse sepsis model and human fibroblast cell culture system. Male BALB-C mice of 8 to 12 weeks as a sepsis animal model were used to evaluate pathology by cecal ligation and puncture (CLP). Analysis of the distribution of fibroblasts using S100 calcium-binding protein, etc. showed an increase in fibroblasts in the lungs and the atrial fascia interface in the time course after CLP. In the study of cell culture of human fibroblast IMR-90, it was possible to evaluate that the β receptor activation by catecholamine and thrombin activity in systemic inflammation are strongly involved in fibroblast proliferation. It was revealed that fibroblast proliferation and organization was observed in association with inflammation of sepsis in the lung and heart.
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Report
(5 results)
Research Products
(15 results)
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[Journal Article] Anti-Inflammatory Profile of Levosimendan in Cecal Ligation-Induced Septic Mice and in Lipopolysaccharide-Stimulated Macrophages.2015
Author(s)
Qiang Wang, Hiroki Yokoo, Michinori Takashina, Kimimasa Sakata, Wakana Ohashi, Lobna A Abdelzaher, Takahiro Imaizumi, Takuya Sakamoto, Kohshi Hattori, Naoyuki Matsuda, Yuichi Hattori
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Journal Title
Critical Care Medicine
Volume: 43巻,11号
Issue: 11
Pages: e508-e520
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Inflammation-induced endothelial cell-derived extracellukar vesicles modulate the cellular status of pericytes.2015
Author(s)
Yamamoto S., Niida S., Azuma E., Yanagibashi T., Muramatsu M., Huang TT., Sagara H., Higaki S., Ikutani M., Nagai Y., Takatsu K., Miyazaki K., Hamashima T., Mori H., Matsuda N., Ishii Y., Sasahara M.
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Journal Title
Scientific Reports
Volume: 5
Issue: 1
Pages: 8505-8505
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The Japanese guidelines for the management of sepsis.2014
Author(s)
Oda S, Aibiki M, Ikeda T, Imaizumi H, Endo S, Ochiai R, Kotani J, Shime N, Nishida O, Noguchi T, Matsuda N, Hirasawa H; Sepsis Registry Committee of The Japanese Society of Intensive Care Medicine.
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Journal Title
J Intensive Care
Volume: 28
Issue: 1
Pages: 55-55
DOI
Related Report
Peer Reviewed / Open Access
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