How autophagy regulate periodontal bacterium

• Project/Area Number: 25293372
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Osaka University
• Principal Investigator: Noda Takeshi 大阪大学, 歯学研究科(研究院), 教授 (00290908)
• Co-Investigator(Kenkyū-buntansha): 天野 敦雄 大阪大学, 歯学研究科(研究院), 教授 (50193024) ; 稲葉 裕明 大阪大学, 歯学研究科(研究院), 助教 (70359850)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: オートファジー / エンドサイトーシス / 歯周病

Outline of Final Research Achievements

Periodondal disease is caused by excessive immunological reaction in epitheliumm, macrophage, and osteoclast in the gums and alveolar bone, which is caused by colonization of bacterium such as Porohyromonus gingibalis. In this study we revealed that Porohyromonus gingibalis invasion is associated with LYST mediated endocytosis.

Research Products

• [Journal Article] Quantitative assay of macroautophagy using Pho8d60 assay and GFP-cleavage assay in yeast (2017)
  • Author(s): Araki Y., Kira S., and Noda T*
  • Journal Title: Methods in Enzymolgy Volume: 588 Pages: 307-321
  • DOI: 10.1016/bs.mie.2016.10.027
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiae (2017)
  • Author(s): Ogasawara Y, Kira S , Mukai Y, Noda T*, Yamamoto A*
  • Journal Title: Biol Open Volume: 6 Pages: 35-40
  • DOI: 10.1242/bio.022053
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Atg9A Trafficking Through the Recycling Endosomes is Required for Autophagosome Formation (2016)
  • Author(s): Imai K, Hao F, Fujita N, Tsuji Y, Oe Y, Araki Y, Hamasaki M, Noda T*, and Yoshimori T*
  • Journal Title: J Cell Sci Volume: 129 Issue: 20 Pages: 3781-3791
  • DOI: 10.1242/jcs.196196
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The PtdIns3-phosphatase MTMR3 interacts with mTORC1 and suppresses its activity. (2016)
  • Author(s): Hao F, Itoh T, Morita E, Shirahama-Noda K, Yoshimori T, Noda T.
  • Journal Title: FEBS Lett. Volume: 509 Issue: 1 Pages: 161-173
  • DOI: 10.1002/1873-3468.12048
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dynamic relocation of the TORC1–Gtr1/2–Ego1/2/3 complex is regulated by Gtr1 and Gtr2 (2015)
  • Author(s): Kira S, Kumano Y, Ukai H, Takeda E, Matsuura A, Noda T
  • Journal Title: Molecular Biology of the cell Volume: 27 Issue: 2 Pages: 382-386
  • DOI: 10.1091/mbc.e15-07-0470
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Reciprocal conversion of Gtr1 and Gtr2 nucleotide- binding states by Npr2-Npr3 inactivates TORC1 and induces autophagy. (2014)
  • Author(s): Kira S, Tabata K, Shirahama-Noda K, Nozoe A, Yoshimori T, Noda T.
  • Journal Title: Autophagy. Volume: 10 Issue: 9 Pages: 1565-78
  • DOI: 10.4161/auto.29397
  • Peer Reviewed / Open Access
• [Journal Article] Porphyromonas gingivalis promotes invasion of oral squamous cell carcinoma through induction of proMMP9 and its activation (2014)
  • Author(s): Inaba H, Sugita H, Kuboniwa M, Iwai S, Hamada M, Noda T, Morisaki I, Lamont RJ, Amano A
  • Journal Title: Cellular Microbiology Volume: 16(1) Issue: 1 Pages: 131-145
  • DOI: 10.1111/cmi.12211
  • Peer Reviewed / Open Access
• [Journal Article] TRAPPIII is responsible for the vesicular transport from early endosomes to the Golgi apparatus that facilitates Atg9 cycling in autophagy (2013)
  • Author(s): Shirahama-Noda K, Kira S, Yoshimori T, Noda T
  • Journal Title: J Cell Sci. Volume: 126 Pages: 4963-73
  • DOI: 10.1242/jcs.131318
  • Peer Reviewed / Open Access
• [Journal Article] Recruitment of the autophagic machinery to endosomes during infection is mediated by ubiquitin (2013)
  • Author(s): Naonobu Fujita et al.
  • Journal Title: J. Cell Biol. Volume: 203 Issue: 1 Pages: 115-128
  • DOI: 10.1083/jcb.201304188
  • Peer Reviewed / Open Access
• [Presentation] Study of termination mechanism of autophagy under prolonged starvation (2016)
  • Author(s): Noda T.
  • Organizer: the 14th International Congress on Yeasts
  • Place of Presentation: 淡路夢舞台（兵庫県淡路市）
  • Year and Date: 2016-09-11
  • Int'l Joint Research
• [Presentation] 破骨細胞に関わるRabタンパク質の網羅的スクリーニング (2016)
  • Author(s): 野田 和也、伊藤 敬、福田 光則、野田 健司
  • Organizer: 第68回日本細胞生物学会
  • Place of Presentation: 京都テルサ（京都府京都市）
  • Year and Date: 2016-06-15
• [Presentation] The PtdIns3-phosphatase MTMR3 interacts with mTORC1 and suppresses its activity (2016)
  • Author(s): Feike Hao、伊藤 敬、森田 英嗣、野田 佳苗、吉森 保、野田 健司
  • Organizer: 第６８回日本細胞生物学会大会・第11回日本ケミカルバイオロジー学会
  • Place of Presentation: 京都テルサ（京都府京都市）
  • Year and Date: 2016-06-15
• [Presentation] Autophagy Regulation by Gtr-Gia-Tag Network (2016)
  • Author(s): Noda T
  • Organizer: The 4th Sino-Japan Symposium on Autophagy
  • Place of Presentation: 北京、中国
  • Int'l Joint Research / Invited
• [Presentation] 新たなTORC1 制御機構の解明 (2015)
  • Author(s): 鵜飼洋史、吉良新太郎、野田健司
  • Organizer: 第38回分子生 物学会年会・第88回生化学会大会合同大会BMB2015
  • Place of Presentation: 兵庫県神戸市神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] Ego複合体新規サブユニットの解析 (2015)
  • Author(s): 吉良新太郎、熊野佑里、野田健司
  • Organizer: 第38回分子生 物学会年会・第88回生化学会大会合同大会BMB2015
  • Place of Presentation: 兵庫県神戸市神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] 長時間飢餓時のオートファジー停止機構の研究 (2015)
  • Author(s): 野田健司
  • Organizer: 日本細胞生物学会年会シンポジウム
  • Place of Presentation: 東京都江東区タワーホール船堀
  • Year and Date: 2015-07-01