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Supermolecule structure and mechanism of a novel bacterial protein secretion system, type IX secretion system

Research Project

Project/Area Number 25293375
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionNagasaki University

Principal Investigator

NAKAYAMA Koji  長崎大学, 医歯薬学総合研究科(歯学系), 教授 (80150473)

Co-Investigator(Kenkyū-buntansha) IMADA Katsumi  大阪大学, 理学研究科, 教授 (40346143)
Co-Investigator(Renkei-kenkyūsha) NAITO Mariko  長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (20244072)
SHOJI Mikio  長崎大学, 医歯薬学総合研究科(歯学系), 助教 (10336175)
SATO Keiko  長崎大学, 医歯薬学総合研究科(歯学系), 助教 (70410579)
YUKITAKE Hideharu  長崎大学, 医歯薬学総合研究科(歯学系), 技術職員 (30380984)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Keywords歯周病 / タンパク分泌 / 細菌 / 蛋白質分泌 / タンパク質分泌
Outline of Final Research Achievements

We found a novel secretion system, Por secretion system in the periodontal pathogen Porphyromonas gingivalis, which is now called the type IX secretion system (T9SS). We found a ring-shaped structure consisting of PorK and PorN in the membrane fraction of P. gingivalis. We found with mutant study that Tannerella forsythia, Prvotella intermedia and Capnocytophaga ochracea have functional T9SSs. T9SSs are not limited to the oral bacteria but extend to other bacteria belonging to the phylum Bacteroidetes such as Flavobacterium johnsoniae and Cytophaga hutchinsonii known as gliding bacteria. Gliding motility of the bacteria is tightly associated with T9SS. We propose a helical loop track model for gliding motility of Bacteroidetes phylum bacteria.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (18 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (2 results) Journal Article (12 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 12 results,  Acknowledgement Compliant: 3 results,  Open Access: 8 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Int'l Joint Research] Scripps研究所/SLAC国立加速器研究所(米国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] ロンドン大学クイーン・メリー校(英国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] A Distinct Type of Pilus from the Human Microbiome.2016

    • Author(s)
      Xu Q, Shoji M, Shibata S, Naito M, Sato K, Elsliger MA, Grant JC, Axelrod HL, Chiu HJ, Farr CL, Jaroszewski L, Knuth MW, Deacon AM, Godzik A, Lesley SA, Curtis MA, Nakayama K, Wilson IA
    • Journal Title

      Cell

      Volume: 165 Issue: 3 Pages: 690-703

    • DOI

      10.1016/j.cell.2016.03.016

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A two-component system regulates gene expression of the type IX secretion component proteins via an ECF sigma factor2016

    • Author(s)
      門脇知子, 雪竹英治, 内藤真理子, 佐藤啓子, 菊池有一郎, 近藤好夫, 庄子幹郎, 中山浩次
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 23288-23288

    • DOI

      10.1038/srep23288

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Involvement of the type IX secretion system in the gliding motility and biofilm formation of Capnocytophaga ochracea2016

    • Author(s)
      Kita D, Shibata S, Kikuchi Y, Kokubu E, Nakayama K, Saito A, Ishihara K
    • Journal Title

      Appl Environ Microbiol

      Volume: 82 Issue: 6 Pages: 1756-1766

    • DOI

      10.1128/aem.03452-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Involvement of an Skp-Like Protein, PGN_0300, in the Type IX Secretion System of Porphyromonas gingivalis.2015

    • Author(s)
      Taguchi Y, Sato K, Yukitake H, Inoue T, Nakayama M, Naito M, Kondo Y, Kano K, Hoshino T, Nakayama K, Takashiba S, Ohara N.
    • Journal Title

      Infection and Immunity

      Volume: 84 Issue: 1 Pages: 230-40

    • DOI

      10.1128/iai.01308-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Porphyromonas gingivalis and related bacteria: from colonial pigmentation to the type IX secretion system and gliding motility.2015

    • Author(s)
      Nakayama K
    • Journal Title

      J Periodontal Res

      Volume: 50 Issue: 1 Pages: 1-8

    • DOI

      10.1111/jre.12255

    • NAID

      120006986253

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Involvement of the Wbp pathway in the biosynthesis of Porphyromonas gingivalis lipopolysaccharide with anionic polysaccharide.2014

    • Author(s)
      6.Shoji M, Sato K, Yukitake H, Naito M, Nakayama K
    • Journal Title

      Scientific Reports

      Volume: 4 Issue: 1 Pages: 5156-5156

    • DOI

      10.1038/srep05056

    • NAID

      120006986576

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Analysis of a Lys-specific serine endopeptidase secreted via the type IX secretion system in Porphyromonas gingivalis.2014

    • Author(s)
      Nonaka M, Shoji M, Kadowaki T, Sato K, Yukitake H, Naito M, Nakayama K
    • Journal Title

      FEMS Microbiology Letters

      Volume: in press Issue: 1 Pages: 60-68

    • DOI

      10.1111/1574-6968.12426

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lack of a surface layer in Tannerella forsythia mutants deficient in the type IX secretion system.2014

    • Author(s)
      7.Narita Y, Sato K, Yukitake H, Shoji M, Daisuke N, Nagano K, Yoshimura F, Naito M, Nakayama K
    • Journal Title

      Microbiology-SGM

      Volume: 160 Issue: 10 Pages: 2295-2303

    • DOI

      10.1099/mic.0.080192-0

    • NAID

      120006986357

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification and characterization of prokaryotic dipeptidyl-peptidase 5 from Porphyromonas gingivalis.2014

    • Author(s)
      Ohara-Nemoto Y, Rouf SMA, Naito M, Yanase A, Tetsuo F, Ono T, Kobayakawa T, Shimoyama Y, Kimura S, Nakayama K, Saiki K, Konishi K, Nemoto TK.
    • Journal Title

      J. Biol. Chem.

      Volume: 289 Issue: 9 Pages: 5438-5448

    • DOI

      10.1074/jbc.m113.527333

    • NAID

      120006986777

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hemoglobin receptor protein from Porphyromonas gingivalis induces interleukin-8 production in human gingival epithelial cells through stimulation of the mitogen-activated protein kinase and NF-κB signal transduction pathways2014

    • Author(s)
      Fujita Y, Nakayama M, Naito M, Yamachika E, Inoue T, Nakayama K, Iida S, Ohara N.
    • Journal Title

      Infection and Immunity

      Volume: 82 Issue: 1 Pages: 202-211

    • DOI

      10.1128/iai.01140-12

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Helical flow of surface protein required for bacterial gliding motility.2013

    • Author(s)
      102. Nakane D, Sato K, Wada H, McBride MJ, Nakayama K
    • Journal Title

      Proc. Nat. Acd. Sci. USA

      Volume: 110 Issue: 27 Pages: 11145-11150

    • DOI

      10.1073/pnas.1219753110

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of an O-antigen chain length regul tor, WzzP, in Porphyromonas gingivalis.2013

    • Author(s)
      Shoji M, Yukitake H, Sato K, Shibata Y, Naito M, Aduse-Opoku J, Abiko Y, Curtis MA, N kayama K.
    • Journal Title

      Microbiologyopen.Epub

      Volume: 10 Issue: 3 Pages: 383-401

    • DOI

      10.1002/mbo3.84

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 細菌の新しい分泌システムと滑走運動2015

    • Author(s)
      中山浩次
    • Organizer
      第98回日本細菌学会関東支部総会
    • Place of Presentation
      東京歯科大学(東京都千代田区)
    • Year and Date
      2015-10-29
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] The type IX secretion system of Porphyromonas gingivalis2015

    • Author(s)
      Nakayama K
    • Organizer
      PgLONDON2015
    • Place of Presentation
      ロンドン(英国)
    • Year and Date
      2015-05-14
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Porphyromonas gingivalis9型分泌機構の輸送に関与するCTDタンパク質の解析2015

    • Author(s)
      雪竹英治、佐藤啓子、近藤好夫、庄子幹郎、内藤真理子、中山浩次
    • Organizer
      第8回日本細菌学会総会
    • Place of Presentation
      長良川国際会議場(岐阜市)
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] ジンジパインプロテアーゼにおけるIg-like domainの役割2014

    • Author(s)
      雪竹英治、佐藤啓子、中根大介、成田由香、柴田敏史、中山浩次
    • Organizer
      日本細菌学会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2022-01-27  

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