Supermolecule structure and mechanism of a novel bacterial protein secretion system, type IX secretion system

• Project/Area Number: 25293375
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Nagasaki University
• Principal Investigator: NAKAYAMA Koji 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (80150473)
• Co-Investigator(Kenkyū-buntansha): IMADA Katsumi 大阪大学, 理学研究科, 教授 (40346143)
• Co-Investigator(Renkei-kenkyūsha): NAITO Mariko 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (20244072) ; SHOJI Mikio 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (10336175) ; SATO Keiko 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (70410579) ; YUKITAKE Hideharu 長崎大学, 医歯薬学総合研究科(歯学系), 技術職員 (30380984)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
• Keywords: 歯周病 / タンパク分泌 / 細菌 / 蛋白質分泌 / タンパク質分泌

Outline of Final Research Achievements

We found a novel secretion system, Por secretion system in the periodontal pathogen Porphyromonas gingivalis, which is now called the type IX secretion system (T9SS). We found a ring-shaped structure consisting of PorK and PorN in the membrane fraction of P. gingivalis. We found with mutant study that Tannerella forsythia, Prvotella intermedia and Capnocytophaga ochracea have functional T9SSs. T9SSs are not limited to the oral bacteria but extend to other bacteria belonging to the phylum Bacteroidetes such as Flavobacterium johnsoniae and Cytophaga hutchinsonii known as gliding bacteria. Gliding motility of the bacteria is tightly associated with T9SS. We propose a helical loop track model for gliding motility of Bacteroidetes phylum bacteria.

Research Products

• [Int'l Joint Research] Scripps研究所/SLAC国立加速器研究所(米国)
• [Int'l Joint Research] ロンドン大学クイーン・メリー校(英国)
• [Journal Article] A Distinct Type of Pilus from the Human Microbiome. (2016)
  • Author(s): Xu Q, Shoji M, Shibata S, Naito M, Sato K, Elsliger MA, Grant JC, Axelrod HL, Chiu HJ, Farr CL, Jaroszewski L, Knuth MW, Deacon AM, Godzik A, Lesley SA, Curtis MA, Nakayama K, Wilson IA
  • Journal Title: Cell Volume: 165 Issue: 3 Pages: 690-703
  • DOI: 10.1016/j.cell.2016.03.016
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A two-component system regulates gene expression of the type IX secretion component proteins via an ECF sigma factor (2016)
  • Author(s): 門脇知子, 雪竹英治, 内藤真理子, 佐藤啓子, 菊池有一郎, 近藤好夫, 庄子幹郎, 中山浩次
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 23288-23288
  • DOI: 10.1038/srep23288
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of the type IX secretion system in the gliding motility and biofilm formation of Capnocytophaga ochracea (2016)
  • Author(s): Kita D, Shibata S, Kikuchi Y, Kokubu E, Nakayama K, Saito A, Ishihara K
  • Journal Title: Appl Environ Microbiol Volume: 82 Issue: 6 Pages: 1756-1766
  • DOI: 10.1128/aem.03452-15
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of an Skp-Like Protein, PGN_0300, in the Type IX Secretion System of Porphyromonas gingivalis. (2015)
  • Author(s): Taguchi Y, Sato K, Yukitake H, Inoue T, Nakayama M, Naito M, Kondo Y, Kano K, Hoshino T, Nakayama K, Takashiba S, Ohara N.
  • Journal Title: Infection and Immunity Volume: 84 Issue: 1 Pages: 230-40
  • DOI: 10.1128/iai.01308-15
  • Peer Reviewed / Open Access
• [Journal Article] Porphyromonas gingivalis and related bacteria: from colonial pigmentation to the type IX secretion system and gliding motility. (2015)
  • Author(s): Nakayama K
  • Journal Title: J Periodontal Res Volume: 50 Issue: 1 Pages: 1-8
  • DOI: 10.1111/jre.12255
  • NAID: 120006986253
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of the Wbp pathway in the biosynthesis of Porphyromonas gingivalis lipopolysaccharide with anionic polysaccharide. (2014)
  • Author(s): 6.Shoji M, Sato K, Yukitake H, Naito M, Nakayama K
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 5156-5156
  • DOI: 10.1038/srep05056
  • NAID: 120006986576
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Analysis of a Lys-specific serine endopeptidase secreted via the type IX secretion system in Porphyromonas gingivalis. (2014)
  • Author(s): Nonaka M, Shoji M, Kadowaki T, Sato K, Yukitake H, Naito M, Nakayama K
  • Journal Title: FEMS Microbiology Letters Volume: in press Issue: 1 Pages: 60-68
  • DOI: 10.1111/1574-6968.12426
  • Peer Reviewed / Open Access
• [Journal Article] Lack of a surface layer in Tannerella forsythia mutants deficient in the type IX secretion system. (2014)
  • Author(s): 7.Narita Y, Sato K, Yukitake H, Shoji M, Daisuke N, Nagano K, Yoshimura F, Naito M, Nakayama K
  • Journal Title: Microbiology-SGM Volume: 160 Issue: 10 Pages: 2295-2303
  • DOI: 10.1099/mic.0.080192-0
  • NAID: 120006986357
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification and characterization of prokaryotic dipeptidyl-peptidase 5 from Porphyromonas gingivalis. (2014)
  • Author(s): Ohara-Nemoto Y, Rouf SMA, Naito M, Yanase A, Tetsuo F, Ono T, Kobayakawa T, Shimoyama Y, Kimura S, Nakayama K, Saiki K, Konishi K, Nemoto TK.
  • Journal Title: J. Biol. Chem. Volume: 289 Issue: 9 Pages: 5438-5448
  • DOI: 10.1074/jbc.m113.527333
  • NAID: 120006986777
  • Peer Reviewed / Open Access
• [Journal Article] Hemoglobin receptor protein from Porphyromonas gingivalis induces interleukin-8 production in human gingival epithelial cells through stimulation of the mitogen-activated protein kinase and NF-κB signal transduction pathways (2014)
  • Author(s): Fujita Y, Nakayama M, Naito M, Yamachika E, Inoue T, Nakayama K, Iida S, Ohara N.
  • Journal Title: Infection and Immunity Volume: 82 Issue: 1 Pages: 202-211
  • DOI: 10.1128/iai.01140-12
  • Peer Reviewed
• [Journal Article] Helical flow of surface protein required for bacterial gliding motility. (2013)
  • Author(s): 102. Nakane D, Sato K, Wada H, McBride MJ, Nakayama K
  • Journal Title: Proc. Nat. Acd. Sci. USA Volume: 110 Issue: 27 Pages: 11145-11150
  • DOI: 10.1073/pnas.1219753110
  • Peer Reviewed
• [Journal Article] Identification of an O-antigen chain length regul tor, WzzP, in Porphyromonas gingivalis. (2013)
  • Author(s): Shoji M, Yukitake H, Sato K, Shibata Y, Naito M, Aduse-Opoku J, Abiko Y, Curtis MA, N kayama K.
  • Journal Title: Microbiologyopen.Epub Volume: 10 Issue: 3 Pages: 383-401
  • DOI: 10.1002/mbo3.84
  • Peer Reviewed
• [Presentation] 細菌の新しい分泌システムと滑走運動 (2015)
  • Author(s): 中山浩次
  • Organizer: 第98回日本細菌学会関東支部総会
  • Place of Presentation: 東京歯科大学（東京都千代田区）
  • Year and Date: 2015-10-29
  • Invited
• [Presentation] The type IX secretion system of Porphyromonas gingivalis (2015)
  • Author(s): Nakayama K
  • Organizer: PgLONDON2015
  • Place of Presentation: ロンドン（英国）
  • Year and Date: 2015-05-14
  • Int'l Joint Research / Invited
• [Presentation] Porphyromonas gingivalis９型分泌機構の輸送に関与するCTDタンパク質の解析 (2015)
  • Author(s): 雪竹英治、佐藤啓子、近藤好夫、庄子幹郎、内藤真理子、中山浩次
  • Organizer: 第8回日本細菌学会総会
  • Place of Presentation: 長良川国際会議場（岐阜市）
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] ジンジパインプロテアーゼにおけるIg-like domainの役割 (2014)
  • Author(s): 雪竹英治、佐藤啓子、中根大介、成田由香、柴田敏史、中山浩次
  • Organizer: 日本細菌学会
  • Place of Presentation: タワーホール船堀（東京都江戸川区）