Elucidation of the mechanism of systemic diseases by a periodontal pathogen in the intracellular transport
| Project/Area Number |
25293383
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Nagasaki University |
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Principal Investigator |
TSUKUBA Takayuki 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (30264055)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Koji 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (80150473)
OKAMOTO Kuniaki 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (10311846)
NISHISHITA Kazushisa 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (20237697)
SAKAI Eiko 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (10176612)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | Porphyromonas gingivalis / NAFLD / lipid droplets / 歯周病細菌 / 全身疾患 / 歯周病原細菌 / 宿主細胞内輸送 |
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| Outline of Final Research Achievements |
Recent studies have shown that infection with Porphyromonas gingivalis, a major periodontal pathogen, hastens the progression of non-alcoholic fatty liver disease (NAFLD). However, the intracellular fate of P. gingivalis in hepatocytes remains unknown. Here, using oleic-acid-induced HepG2 cells as an in vitro model for NAFLD, we found that lipid droplets increased the existence of P. gingivalis in the cells at an early phase of infection. Confocal microscopic analysis revealed that lipid droplets affected the formation of autolysosomes in infected cells. Thus, lipid droplets affect the elimination of P. gingivalis in HepG2 cells by altering the autophagy-lysosome system.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Kadowaki T, Kido MA, Hatakeyama J, Okamoto K, Tsukuba T, Yamamoto K. Defective adipose tissue development associated with hepatomegaly in cathepsin E-deficient mice fed a high-fat diet.2014
Author(s)
Kadowaki T, Kido MA, Hatakeyama J, Okamoto K, Tsukuba T, Yamamoto K.
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Journal Title
Biochem Biophys Res Commun.
Volume: 446(1)
Issue: 1
Pages: 212-217
DOI
Related Report
Peer Reviewed
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[Journal Article] Cathepsin E in mammary gland: Repression of cathepsin E expression increases the risk of mammary carcinogenesis and links to poor prognosis in breast cancer.2013
Author(s)
Tomoyo Kawakubo, Atsushi Yasukochi, Tatsuya Toyama, Satoru Takahashi, Kuniaki Okamoto, Takayuki Tsukuba, Seiji Nakamura, Yasuhiko Ozaki, Koichi Nishigaki, Hiroko Yamashita, and Kenji Yamamoto
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Journal Title
Carcinogenesis
Volume: 00
Issue: 3
Pages: 1-13
DOI
Related Report
Peer Reviewed / Open Access
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