| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Outline of Final Research Achievements |
The dental pulp stem cells (DPSCs) isolated from human dental pulp tissue using colony isolation method expressed typical mesenchymal stem cell markers, and they possessed potential to differentiate into various tissues/cells, indicating that DPSCs isolated by colony isolation method may be a good source for regenerative medicine. 3-D spheroid culture of isolated DPSCs stimulated the integrin signaling, which further induced differentiation to hard tissue forming cells. On the other hand, 3-D collagen culture prohibited their differentiation to hard tissue forming cells. Control of the cellular signaling network including integrin signaling and application of appropriate scaffold may pave the way for a new approach of whole pulp tissue regeneration.
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