Establishment of dental pulp stem cell-isolation method and clinical approach of dental pulp tissue regeneration using dental pulp stem cells by signaling network control

• Project/Area Number: 25293386
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KAWASHIMA Nobuyuki 東京医科歯科大学, 大学院医歯学総合研究科, 講師 (60272605)
• Co-Investigator(Kenkyū-buntansha): 関矢 一郎 東京医科歯科大学, 統合研究機構, 教授 (10345291)
• Project Period (FY): 2013-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000); Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
• Keywords: dental pulp stem cells / stem cell isolation / 3-D culture / spheroid / integrin signaling / differentiation / collagen scafold / dental pulp regeneration / 歯髄幹細胞 / 間葉系幹細胞 / ステムネス / 三次元培養 / スフェロイド / 硬組織形成細胞 / 分化誘導 / 硬組織再生 / 間葉系幹細胞マーカー / コロニー形成能 / 硬組織形成 / 組織再生 / インテグリンシグナル / 未分化間葉細胞 / シグナルネットワーク / 細胞分化 / インテグリン / 骨再生 / 象牙質再生 / 幹細胞の分離 / スキャフォールドフリー / 再生医療

Outline of Final Research Achievements

The dental pulp stem cells (DPSCs) isolated from human dental pulp tissue using colony isolation method expressed typical mesenchymal stem cell markers, and they possessed potential to differentiate into various tissues/cells, indicating that DPSCs isolated by colony isolation method may be a good source for regenerative medicine. 3-D spheroid culture of isolated DPSCs stimulated the integrin signaling, which further induced differentiation to hard tissue forming cells. On the other hand, 3-D collagen culture prohibited their differentiation to hard tissue forming cells. Control of the cellular signaling network including integrin signaling and application of appropriate scaffold may pave the way for a new approach of whole pulp tissue regeneration.

Research Products

• [Journal Article] EDTA Treatment for Sodium Hypochlorite?treated Dentin Recovers Disturbed Attachment and Induces Differentiation of Mouse Dental Papilla Cells (2018)
  • Author(s): Hashimoto Kentaro、Kawashima Nobuyuki、Ichinose Shizuko、Nara Keisuke、Noda Sonoko、Okiji Takashi
  • Journal Title: Journal of Endodontics Volume: 44 Issue: 2 Pages: 256-262
  • DOI: 10.1016/j.joen.2017.11.003
  • Peer Reviewed
• [Journal Article] Properties of Dental Pulp?derived Mesenchymal Stem Cells and the Effects of Culture Conditions (2017)
  • Author(s): Kawashima Nobuyuki、Noda Sonoko、Yamamoto Mioko、Okiji Takashi
  • Journal Title: Journal of Endodontics Volume: 43 Issue: 9 Pages: S31-S34
  • DOI: 10.1016/j.joen.2017.06.004
  • Peer Reviewed
• [Journal Article] Transient receptor potential melastatin (TRPM) 8 is expressed in freshly isolated native human odontoblasts (2017)
  • Author(s): Tazawa Kento、Ikeda Hideharu、Kawashima Nobuyuki、Okiji Takashi
  • Journal Title: Archives of Oral Biology Volume: 75 Pages: 55-61
  • DOI: 10.1016/j.archoralbio.2016.12.007
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effect of a pH-modified Low-concentration EDTA Irrigant on Smear Layer Removal and Dentin Demineralization (2017)
  • Author(s): 川島 伸之、戸村 淳嗣、横田 兼欣、興地 隆史
  • Journal Title: The Japanese Journal of Conservative Dentistry Volume: 60 Issue: 1 Pages: 32-39
  • DOI: 10.11471/shikahozon.60.32
  • NAID: 130005402099
  • ISSN: 0387-2343, 2188-0808
  • Peer Reviewed
• [Journal Article] Odontoblasts: specialized hard-tissue-forming cells in the dentin-pulp complex (2016)
  • Author(s): Kawashima N and Okiji T
  • Journal Title: Congenit Anom (Kyoto) Volume: 56 Issue: 4 Pages: 144-153
  • DOI: 10.1111/cga.12169
  • NAID: 130008079156
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Osr2 (odd-skipped related 2)はマウス歯乳頭細胞の硬組織形成細胞への分化を促進する (2016)
  • Author(s): 川島伸之、齋藤 正寛、興地隆史
  • Journal Title: 歯内療法学会誌 Volume: 37 Pages: 38-44
  • NAID: 130006108702
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Transplantation of autologous synovial mesenchymal stem cells promotes meniscus regeneration in aged primates. (2016)
  • Author(s): Kondo S, Muneta T, Nakagawa Y, Koga H, Watanabe T, Tsuji K, Sotome S, Okawa A, Kiuchi S, Ono H, Mizuno M, Sekiya I
  • Journal Title: J Orthop Res Volume: in press Issue: 6 Pages: 1274-1282
  • DOI: 10.1002/jor.23211
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cartilage derived from bone marrow mesenchymal stem cells expresses lubricin in vitro and in vivo (2016)
  • Author(s): Yusuke Nakagawa, Takeshi Muneta, Koji Otabe, Nobutake Ozeki, Mitsuru Mizuno, Mio Udo, Ryusuke Saito, Katsuaki Yanagisawa, Shizuko Ichinose, Hideyuki Koga, Kunikazu Tsuji and Ichiro Sekiya
  • Journal Title: PLoS ONE Volume: 11 Issue: 2 Pages: e0148777-e0148777
  • DOI: 10.1371/journal.pone.0148777
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cathepsin K inhibitor regulates inflammation and bone destruction in experimentally induced rat periapical lesions (2015)
  • Author(s): Suzuki N, Takimoto K, Kawashima N
  • Journal Title: Journal of Endodontics Volume: 41 Issue: 9 Pages: 1474-1479
  • DOI: 10.1016/j.joen.2015.04.013
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Synovial mesenchymal stem cells promote meniscus regeneration augmented by an autologous Achilles tendon graft in a rat partial meniscus defect model. (2015)
  • Author(s): Ozeki N, Muneta T, Matsuta S, Koga H, Nakagawa Y, Mizuno M, Tsuji K, Mabuchi Y, Akazawa C, Kobayashi E, Saito T, Sekiya I.
  • Journal Title: Stem Cells. Volume: 33(6) Issue: 6 Pages: 1927-38
  • DOI: 10.1002/stem.2030
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Purified Human Synovium Mesenchymal Stem Cells as a Good Resource for Cartilage Regeneration. (2015)
  • Author(s): Ogata Y, Mabuchi Y, Yoshida M, Suto EG, Suzuki N, Muneta T, Sekiya I, Akazawa C.
  • Journal Title: PLoS One. Volume: 10(6) Issue: 6 Pages: e0129096-e0129096
  • DOI: 10.1371/journal.pone.0129096
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evaluation of a behind-remnant approach for femoral tunnel creation in remnant-preserving double-bundle anterior cruciate ligament reconstruction - Comparison with a standard approach. (2015)
  • Author(s): Koga H, Muneta T, Yagishita K, Watanabe T, Mochizuki T, Horie M, Nakamura T, Otabe K, Sekiya I.
  • Journal Title: Knee Volume: in press Issue: 3 Pages: 249-255
  • DOI: 10.1016/j.knee.2015.02.017
  • Peer Reviewed
• [Journal Article] Synovial mesenchymal stem cells promote healing after meniscal repair in microminipigs. (2015)
  • Author(s): Nakagawa Y, Muneta T, Kondo S, Mizuno M, Takakuda K, Ichinose S, Tabuchi T, Koga H, Tsuji K, Sekiya I.
  • Journal Title: Osteoarthritis Cartilage. Volume: in press Issue: 6 Pages: 1007-1017
  • DOI: 10.1016/j.joca.2015.02.008
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mouse synovial mesenchymal stem cells increase in yield with knee inflammation. (2015)
  • Author(s): Matsukura Y, Muneta T, Tsuji K, Miyatake K, Yamada J, Abula K, Koga H, Tomita M, Sekiya I.
  • Journal Title: J Orthop Res. Volume: 33 Issue: 2 Pages: 246-53
  • DOI: 10.1002/jor.22753
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mid- to long-term results of single-bundle versus double-bundle anterior cruciate ligament reconstruction: randomized controlled trial. (2015)
  • Author(s): Koga H, Muneta T, Yagishita K, Watanabe T, Mochizuki T, Horie M, Nakamura T, Otabe K, Sekiya I.
  • Journal Title: Arthroscopy Volume: 31 Issue: 1 Pages: 69-76
  • DOI: 10.1016/j.arthro.2014.07.020
  • Peer Reviewed
• [Journal Article] Transcription factor Mohawk controls tenogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo. (2015)
  • Author(s): Otabe K, Nakahara H, Hasegawa A, Matsukawa T, Ayabe F, Onizuka N, Inui M, Takada S, Ito Y, Sekiya I, Muneta T, Lotz M, Asahara H.
  • Journal Title: J Orthop Res. Volume: 33 Issue: 1 Pages: 1-8
  • DOI: 10.1002/jor.22750
  • Peer Reviewed / Open Access
• [Journal Article] Elimination of BMP7 from the developing limb mesenchyme leads to articular cartilage degeneration and synovial inflammation with increased age. (2015)
  • Author(s): Abula K, Muneta T, Miyatake K, Yamada J, Matsukura Y, Inoue M, Sekiya I, Graf D, Economides AN, Rosen V, Tsuji K.
  • Journal Title: FEBS Lett. Volume: in press Issue: 11 Pages: 197-200
  • DOI: 10.1016/j.febslet.2015.04.004
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Periostin is a negative regulator of mineralization in the dental pulp tissue (2014)
  • Author(s): Zhou M, Kawashima N, Suzuki N, Yamamoto M, Ohnishi O, Katsube K, Tanabe H, Kudo A, Saito M, Suda H
  • Journal Title: Odontology Volume: (in press) Issue: 2 Pages: 152-9
  • DOI: 10.1007/s10266-014-0152-7
  • Peer Reviewed / Open Access
• [Journal Article] Down-regulation of Inflammatory Mediator Synthesis and Infiltration of Inflammatory Cells by MMP-3 in Experimentally-induced Rat Pulpitis (2014)
  • Author(s): K Takimoto, N Kawashima, N Suzuki, Y Koizumi, M Yamamoto, M Nakashima, H Suda
  • Journal Title: J Endod Volume: (in press) Issue: 9 Pages: 1404-9
  • DOI: 10.1016/j.joen.2014.04.001
  • Peer Reviewed
• [Journal Article] Three-dimensional spheroid culture promotes odonto/osteoblastic differentiation of dental pulp cells (2014)
  • Author(s): M Yamamoto, N Kawashima, N Takashino, Y Koizumi, K Takimoto, N Suzuki, M Saito, and H Suda
  • Journal Title: Arch Oral Biol Volume: 59 Issue: 3 Pages: 310-317
  • DOI: 10.1016/j.archoralbio.2013.12.006
  • Peer Reviewed
• [Journal Article] Meniscus regeneration by syngeneic, minor mismatched, and major mismatched transplantation of synovial mesenchymal stem cells in a rat model. (2014)
  • Author(s): Okuno M, Muneta T, Koga H, Ozeki N, Nakagawa Y, Tsuji K, Yoshiya S, Sekiya I.
  • Journal Title: J Orthop Res. Volume: in press Issue: 7 Pages: 928-36
  • DOI: 10.1002/jor.22614
  • Peer Reviewed / Open Access
• [Journal Article] Wnt11 expression in rat dental pulp and promotional effects of Wnt signaling on odontoblast differentiation (2013)
  • Author(s): Y Koizumi, N Kawashima, M Yamamoto, K Takimoto, M Zhou, N Suzuki, M Saito, H Harada, H Suda
  • Journal Title: Congenital Anomalies Volume: 53 Issue: 3 Pages: 101-108
  • DOI: 10.1111/cga.12011
  • Peer Reviewed
• [Journal Article] Kinetics of Th17-related cytokine expression in experimentally induced rat periapical lesions (2012)
  • Author(s): S Wei, N Kawashima, N Suzuki, J Xu, S Takahashi, M Zhou, Y Koizumi, H Suda
  • Journal Title: Aust Endod J Volume: epub ahead of print Issue: 3 Pages: 1-8
  • DOI: 10.1111/j.1747-4477.2012.00371.x
  • Peer Reviewed
• [Presentation] Meis2 Induces Odonto-/Osteoblastic Differentiation and Mineralization (2017)
  • Author(s): Kawashima N, Noda S, Hashimoto K, Saito M, Okiji T
  • Organizer: IADR/AADR/CADR General Session & Exhibition
  • Int'l Joint Research
• [Presentation] Dense Culture Condition Promotes Human Dental Pulp Stem Cell Differentiation (2017)
  • Author(s): Noda S, Kawashima N, Hashimoto K, Aramaki O, Okiji T
  • Organizer: IADR/AADR/CADR General Session & Exhibition
  • Int'l Joint Research
• [Presentation] ヒト歯髄細胞における炎症性メディエーター産生制御におけるHIF1a の役割 (2017)
  • Author(s): 藤井真由子，川島伸之，興地隆史
  • Organizer: 第59回歯科基礎医学会学術大会
• [Presentation] 培養密度が歯髄幹細胞の幹細胞特性に与える影響 (2017)
  • Author(s): 野田園子、川島伸之、橋本健太郎、山本弥生子、荒牧音、田上順次、興地隆史
  • Organizer: 第146回日本歯科保存学会2017年春季学術大会
• [Presentation] Mineral trioxide aggregate はLPS刺激マクロファージの機能をcalcium-sensing receptor を介して調節する (2017)
  • Author(s): 倉本将司、川島伸之、野崎浩佑、Bakhit AY、奈良圭介、藤井真由子、橋本健太郎、野田園子、興地隆史
  • Organizer: 第146回日本歯科保存学会2017年春季学術大会
• [Presentation] Strontium ranelate evoked proliferation and mineralization of mouse dental papillae cells is mediated via calcium sensing receptor (2017)
  • Author(s): Bakhit AY, Kawashima N, Hashimoto K, Noda S, Nara S, Okiji T
  • Organizer: 第147回日本歯科保存学会2017年秋季学術大会
• [Presentation] Rescue of mouse dental papillae cell-attachment to sodium hypochlorite-treated dentin disks by EDTA treatment (2016)
  • Author(s): Kawashima N, Hashimoto K, Okiji T
  • Organizer: The 49th Scientific meeting of Korean Academy of Endodontics & The 14th JAE-KAE Joint Scientific Meeting
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2016-11-19
  • Int'l Joint Research
• [Presentation] 歯髄幹細胞の三次元培養における幹細胞マーカーの発現動態 (2016)
  • Author(s): 川島伸之 山本弥生子 野田園子 橋本健太郎 興地隆史
  • Organizer: 第23回日本歯科医学会総会
  • Place of Presentation: 福岡国際会議場, 福岡
  • Year and Date: 2016-10-21
• [Presentation] Properties of dental pulp derived-mesenchymal stem cells and effects of culture conditions (2016)
  • Author(s): Kawashima N, Noda S, Hashimoto K, Yamamoto M, Okiji T
  • Organizer: IADR Pulp Biology and Regeneration Group Satellite Symposium
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2016-06-26
  • Int'l Joint Research / Invited
• [Presentation] Effects of confluent culture condition on dental pulp stem cell surface markers, proliferation, gene expression (2016)
  • Author(s): Noda S, Kawashima N, Hashimoto K, Aramaki O, Yamamoto M, Okiji T
  • Organizer: IADR Pulp Biology and Regeneration Group Satellite Symposium
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Action of Strontium Ranelate on the Dental-Pulp Complex (2016)
  • Author(s): Bakhit A, Kawashima N, Yamamoto M, Hashimoto K, Noda S, Nara K, Okiji T
  • Organizer: IADR Pulp Biology and Regeneration Group Satellite Symposium
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Recovery effects of EDTA treatment on attachment and differentiation of mouse dental pulp cultured on NaOCl-treated dentin (2016)
  • Author(s): K. Hashimoto, N. Kawashima, K. Nara, S. Noda, M. Saito, T. Okiji
  • Organizer: IADR Pulp Biology and Regeneration Group Satellite Symposium
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] 歯髄・歯肉由来の間葉系幹細胞の特性と硬組織再生 (2015)
  • Author(s): 川島伸之、興地隆史
  • Organizer: 日本歯科基礎医学会サテライトシンポジウム
  • Place of Presentation: 朱鷺メッセ（新潟・新潟）
  • Year and Date: 2015-09-11
  • Invited
• [Presentation] Osr2（Odd-skipped related 2）はマウス歯乳頭細胞の硬組織形成細胞への分化を促進する (2015)
  • Author(s): 川島伸之、橋本健太郎、山本弥生子、辺見浩一、鈴木規元、興地隆史
  • Organizer: 日本歯内療法学会学術大会
  • Place of Presentation: 鶴見大学記念館（神奈川・横浜）
  • Year and Date: 2015-07-11
• [Presentation] 歯髄組織および歯肉組織より得られた間葉系幹細胞の硬組織形成細胞への分化能の比較 (2015)
  • Author(s): 川島伸之、山本弥生子、橋本健太郎、Alumddin Bakhit、小泉悠、辺見浩一、大井智恵、鈴木規元、興地隆史
  • Organizer: 日本歯科保存学会春季大会
  • Place of Presentation: 北九州国際会議場（北九州・小倉）
  • Year and Date: 2015-06-25
• [Presentation] Intracanal medicamen (2015)
  • Author(s): N. Kawashima
  • Organizer: APEC
  • Place of Presentation: Amman (Jordan)
  • Year and Date: 2015-04-08
  • Int'l Joint Research / Invited
• [Presentation] Mineralizing Properties of Gingiva-derived Mesenchymal Stem Cells Cultured in 3D-condition (2015)
  • Author(s): N. Kawashima, K. Hashimoto, A. Y. Bakhit, M. Yamamoto, Y. Koizumi, S. Takahashi, C. Ohi, N. Suzuki
  • Organizer: IADR/AADR/CADR General Session
  • Place of Presentation: Hynes Convention Center, Boston, MA, USA
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] ALPase Activity of Pulp Cells on EDTA and NaClO-treated Dentin (2015)
  • Author(s): K. Hashimoto, N. Kawashima, A. Y. Bakhit, M. Yamamoto, Y. Koizumi, S. Takahashi, C. Ohi, N. Suzuki
  • Organizer: IADR/AADR/CADR General Session
  • Place of Presentation: Hynes Convention Center, Boston, MA, USA
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] 歯内療法から考える歯根の発生とその異常 (2014)
  • Author(s): 川島伸之
  • Organizer: 第56回歯科基礎医学会学術大会 サテライトシンポジウム
  • Place of Presentation: 福岡国際会議場、福岡
  • Year and Date: 2014-09-25
  • Invited
• [Presentation] one Tissue Regeneration Using the Spheroid Cultured Dental Pulp Stem Cells (2014)
  • Author(s): Kawashima N and Suzuki N
  • Organizer: Annual Meeting of American Association of Endodontists
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2014-04-30 – 2014-05-03
• [Presentation] Importance of Differential Diagnosis in Keratocystic Odontogenic Tumor (2013)
  • Author(s): Kawashima N, Suda H et.al.
  • Organizer: Asian Pacific Endodontic Conference
  • Place of Presentation: Tokyo International Forum 東京
• [Presentation] Suppressive Regulation of Bone Destruction in Rat Periapical Lesions by Cathepsin K (2013)
  • Author(s): Suzuki N, Kawashima N, et al.
  • Organizer: Asian Pacific Endodontic Conference
  • Place of Presentation: Tokyo International Forum 東京
• [Presentation] Down-regulation of Inflammatory Mediator Synthesis and Infiltration of Immunocompetent Cells in Rat Experimental Pulpitis by MMP-3 (2013)
  • Author(s): Takimoto K, Kawashima N, et al.
  • Organizer: Asian Pacific Endodontic Conference
  • Place of Presentation: Tokyo International Forum 東京
• [Presentation] 3-D Spheroid Culture Method Promotes Odonto-/Osteo-blastic Differentiation of Dental Pulp Cells (2013)
  • Author(s): Yamamoto M, Kawashima N, et al.
  • Organizer: Asian Pacific Endodontic Conference
  • Place of Presentation: Tokyo International Forum 東京
• [Presentation] Effects of Nano-bubbles on Enterococcus faecalis - Transmission Electron Microscopy Investigation (2013)
  • Author(s): Henmi K, Kawashima N, et al.
  • Organizer: Asian Pacific Endodontic Conference
  • Place of Presentation: Tokyo International Forum 東京
• [Presentation] 歯髄幹細胞を用いた再生医療の現状 (2013)
  • Author(s): 川島伸之
  • Organizer: 日本歯科保存学会2013年春季学術大会
  • Place of Presentation: 福岡国際会議場 福岡
  • Invited
• [Presentation] ヒト歯髄幹細胞の三次元培養による象牙芽細胞・骨芽細胞マーカーの変動 (2013)
  • Author(s): 川島伸之、山本弥生子、瀧本晃陽、小泉悠、鈴木規元、須田英明
  • Organizer: 日本歯科保存学会2013年春季学術大会
  • Place of Presentation: 福岡国際会議場 福岡
• [Presentation] スフェロイド培養による歯髄細胞の特性変化の検討 (2013)
  • Author(s): 山本弥生子、川島伸之、鈴木規元、小泉悠、瀧本晃陽、須田英明、斉藤正寛
  • Organizer: 日本歯科保存学会2013年春季学術大会
  • Place of Presentation: 福岡国際会議場 福岡
• [Presentation] ラット臼歯歯髄における樹状細胞のサブポピュレーション (2013)
  • Author(s): 荒牧音、島田康史、大槻昌幸、田上順次、川島伸之、鈴木規元、須田英明
  • Organizer: 日本歯科保存学会2013年春季学術大会
  • Place of Presentation: 福岡国際会議場 福岡
• [Presentation] Periostin: a negative regulator of mineralization in the dental pulp tissue (2013)
  • Author(s): Kawashima N, Mengu Z, Yamamoto M, Koizumi Y, Takimoto K, Onishi K, Suzuki N, Katsube K, Saito M, Harada H, Suda H
  • Organizer: 16th ESE Biennial Congress
  • Place of Presentation: Lisbon ポルトガル
• [Presentation] Spheroid culture induces odonto-/osteoblastic differentiation of dental pulp cells through integrin signaling (2013)
  • Author(s): Yamamoto M, Kawashima N, Koizumi Y, Takimoto K, Onishi K, Suzuki N, Katsube K, Saito M, Harada H, Suda H
  • Organizer: 16th ESE Biennial Congress
  • Place of Presentation: Lisbon ポルトガル
• [Presentation] Stem Cell Marker Expression in the 3-D Spheroid Cultured Human Dental Pulp Stem Cells (2013)
  • Author(s): Kawashima N, Yamamoto M, Takashino N, Takimoto K, Koizumi Y, Suzuki N, Suda H
  • Organizer: The 140th Scientific meeting of KACD
  • Place of Presentation: 慶州 韓国