|Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
|Outline of Final Research Achievements
Cancer-related microRNA(miRNA) has been known in many carcinomas and the possibility of cancer treatment using miRNA has been reported. However, the role of long non coding RNA(IncRNA) in cancer progression is still unknown. The current study, we identified two lncRNA (UCA1 and LINC00256A) by microarray analysis, in order to investigate the role of lncRNA. We made transformed cells by custom shRNA of UCA1 and/or LINC00256A and performed functional analysis. The cells transformed by shUCA1 and/or shLINC00256A showed significantly (P<0.05) decreased invasiveness and migration activities compared with the shMock cells. In addition, we performed microarray analysis using transformed cells and identified target genes. These results indicated that lncRNA may affect tumoral metastasis in OSCCs.