Neurogenic differentiation potential of mouse osteoblastic cell line
| Project/Area Number |
25293415
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Tsurumi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TATEHARA Seiko 鶴見大学, 歯学部, 講師 (90380089)
TOKUYAMA Reiko 鶴見大学, 歯学部, 助教 (20380090)
IDE Shinji 鶴見大学, 歯学部, 助教 (00611998)
TOMIOKA Shigemasa 徳島大学, 大学院医歯薬顎研究部, 准教授 (70188770)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
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| Keywords | 再生医療 / 分化転換 / 機能細胞 / 脊髄損傷 / エピジェネティクス / 組織再生 |
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| Outline of Final Research Achievements |
In this study, we examined the new method for direct reprogramming of adult somatic cells into different cell type by changing environment around cells without gene transfection of transcriptional factors. As a result, mouse osteoblastic cells cultured under the neural conditioned medium were induced transdifferentiation into neural cells. These cells expressed some proteins related neural cells. Moreover, to elucidate the possibility that these cells could be use as the cell source for regenerative medicine of spinal cord, these cells were transplanted with mouse spinal cord injury model. As a result, recovery of motor function of hind limbs was confirmed. Moreover, it is indicated that these cells could produce/secretion some neurotrophic factors. From these findings, it is suggested that adult somatic cells could be a source of regenerative medicine without gene transfection.
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Report
(4 results)
Research Products
(1 results)
