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The tooth-format cellular differentiating regulation from iPSCs

Research Project

Project/Area Number 25293418
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Orthodontics/Pediatric dentistry
Research InstitutionNiigata University

Principal Investigator

Issei Saitoh  新潟大学, 医歯学系, 准教授 (90404540)

Co-Investigator(Kenkyū-buntansha) 山崎 要一  鹿児島大学, 歯学部, 教授 (30200645)
佐藤 正宏  鹿児島大学, 医療ミニブタ先端医療開発研究センター, 教授 (30287099)
齊藤 陽子  新潟大学, 医歯(薬)学総合研究科, 助教 (30404487)
稲田 絵美  鹿児島大学, 医学部・歯学部附属病院, 助教 (30448568)
松山 清  久留米工業高等専門学校, 生物応用化学科, 准教授 (40299540)
野口 洋文  琉球大学, 医学(系)研究科(研究院), 教授 (50378733)
大島 勇人  新潟大学, 医歯学系, 教授 (70251824)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Keywords再生歯学 / iPS細胞 / 歯髄幹細胞 / 乳歯 / 小児歯科学 / 乳歯歯髄
Outline of Final Research Achievements

Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. LEF1 is also an important member of the Wnt/β-catenin signaling pathway that plays important roles in the self-renewal and differentiation of embryonic stem cells. We speculated that LEF1 might function in the stem cells from human exfoliated deciduous teeth (SHED). In this study, we attempted to isolate such LEF1-positive cells and iPS cells from human deciduous dental pulp cells (HDDPCs) by genetic engineering technology, using the human LEF1 promoter.
RT-PCR analysis confirmed the expression of several stem cell markers, including OCT3/4, SOX2, REX1, and NANOG, in LEF1-positive HDDPCs, which could be differentiated into osteoblasts and neuronal cells.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (23 results)

All 2017 2016 2015 2014 2013 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 6 results,  Acknowledgement Compliant: 2 results) Presentation (11 results) (of which Int'l Joint Research: 3 results) Remarks (4 results)

  • [Journal Article] Isolation and characterization of lymphoid enhancer factor-1-positive deciduous dental pulp stem-like cells after transfection with a piggyBac vector containing LEF1 promoter-driven selection markers2017

    • Author(s)
      Tomoya Murakami, Issei Saitoh, Masahiro Sato, Emi Inada, Miki Soda, Masataka Oda, Hisanori Domone, Yoko Iwase, Tadashi Sawami, Kazunari Matsueda, Yutaka Terao, Hayato Ohshima, Hirofumi Noguchi, Haruaki Hayasaki
    • Journal Title

      Archives of Oral Biology

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Efficient CRISPR/Cas9-based gene correction in induced pluripotent stem cells established from fibroblasts of patients with sickle cell disease2016

    • Author(s)
      Masahiro Sato, Issei Saitoh, Emi Inada
    • Journal Title

      Stem Cell Investigation

      Volume: 3 Pages: 78-78

    • DOI

      10.21037/sci.2016.11.05

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs.2016

    • Author(s)
      Sato M, Maeda K, Koriyama M, Inada E, Saitoh I, Miura H, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K.
    • Journal Title

      Int J Mol Sci.

      Volume: 17 Issue: 9 Pages: 1424-1424

    • DOI

      10.3390/ijms17091424

    • NAID

      130007023583

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Choice of feeders is important when first establishing iPSCs derived from primarily cultured human deciduous tooth dental pulp cells.2015

    • Author(s)
      Saitoh I, Inada E, Iwase Y, Noguchi H, Murakami T, Soda M, Kubota N, Hasegawa H, Akasaka E, Matsumoto Y, Oka K, Yamasaki Y, Hayasaki H, Sato M
    • Journal Title

      Cell Medicine

      Volume: 8 Issue: 1-2 Pages: 9-23

    • DOI

      10.3727/215517915x689038

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] PiggyBac transposon-mediated gene delivery efficiently generates stable transfectants derived from cultured primary human deciduous tooth dental pulp cells (HDDPCs) and HDDPC-derived iPS cells.2015

    • Author(s)
      Inada E, Saito I, Watanabe S, Aoki R, Miura H, Ohtsuka M, Murakami T, Sawami T, Yamasaki Y, Sato M
    • Journal Title

      International Journal of Oral Science

      Volume: 7 Issue: 3 Pages: 144-145

    • DOI

      10.1038/ijos.2015.18

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Generation of α-1,3-galactosyltransferase-deficient porcine embryonic fibroblasts by CRISPR/Cas9-mediated knock-in of a small mutated sequence and a targeted toxin-based selection system.2015

    • Author(s)
      Sato M, Kagoshima A, Saitoh I, Inada E, Miyoshi K, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
    • Journal Title

      Domestic Animal Reproduction

      Volume: 50 Issue: 5 Pages: 872-880

    • DOI

      10.1111/rda.12565

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Frontier dental research on iPS cells2013

    • Author(s)
      Arakaki M, Egusa H, Otsu K, Saitoh I, Miura T, Harada H
    • Journal Title

      Journal of Oral Biosciences

      Volume: 55:4 Issue: 4 Pages: 159-164

    • DOI

      10.1016/j.job.2013.08.002

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] STO feeder cells are useful for propagation of primarily cultured human deciduous dental pulp cells in view of elimination of contaminated bacteria and promotion of cellular outgrowth.2013

    • Author(s)
      Murakami T, Saitoh I, Inada E, Kurosawa M, Iwase Y, Noguchi H, Terao Y, Yamasaki Y, Hayasaki H, Sato M
    • Journal Title

      Cell Medicine

      Volume: 7 Issue: 1-2 Pages: 75-81

    • DOI

      10.3727/215517913x674234

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 遺伝子工学的手法による不死化ヒト乳歯歯髄細胞株の樹立と特性解析2017

    • Author(s)
      稲田絵美,齊藤一誠,窪田直子,村上智哉,左右田美樹,澤味 規,松枝一成,早崎治明,山崎要一
    • Organizer
      第55回日本小児歯科学会大会
    • Place of Presentation
      西日本総合展示場(福岡県北九州市)
    • Year and Date
      2017-05-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] The genetic engineering-based isolation of lymphoid enhancer-binding factor-1 (LEF1) positive stem-like cells from human deciduous tooth cell-derived iPSCs2016

    • Author(s)
      Tomoya Murakami, Issei Saitoh, Masahiro Sato, Emi Inada, Miki Soda, Yoko Iwase, Tadashi Sawami, Ayako Suzuki, Hayato Ohshima, Haruaki hayasaki.
    • Organizer
      10th Biennial Conference of the Pediatric Dentistry Association of Asia in conjunction with 54th Annual Conference of the Japanese Society of Pediatric Dentistry
    • Place of Presentation
      東京ドームホテル(東京都文京区)
    • Year and Date
      2016-05-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The genetic engineering-based isolation of lymphoid enhancer-binding factor-1 (LEF1) positive stem-like cells from human deciduous tooth cell-derived iPSCs2016

    • Author(s)
      Tomoya Murakami, Issei Saitoh, Masahiro Sato, Emi Inada, Miki Soda, Yoko Iwase, Tadashi Sawami, Ayako Suzuki, Hayato Ohshima, Haruaki hayasaki
    • Organizer
      10th Biennial Conference of the Pediatric Dentistry Association of Asia in conjunction with 54th Annual Conference of the Japanese Society of Pediatric Dentistry
    • Place of Presentation
      Tokyo Dome Hotel (Tokyo)
    • Year and Date
      2016-05-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] The genetic engineering-based isolation of lymphoid enhancer-binding factor-1 (LEF1) positive stem-like cells from human deciduous tooth cell-derived iPSCs2016

    • Author(s)
      Tomoya Murakami, Issei Saitoh, Masahiro Sato, Emi Inada, Miki Soda, Yoko Iwase, Tadashi Sawami, Ayako Suzuki, Hayato Ohshima, Haruaki hayasaki
    • Organizer
      45rd Annual Meeting & Exhibition of the AADR/ 40h Annual Meeting of the CADR
    • Place of Presentation
      Los Angeles (USA)
    • Year and Date
      2016-03-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ALP as a reliable marker for predicting early reprogramming2016

    • Author(s)
      Miki Soda, Issei Saitoh, Emi Inada, Tomoya Murakami, Ayako Suzuki, Tadashi Sawami, Akiko Kagoshima, Yoko Iwase, Yutaka Terao, Hayato Ohshima, Haruaki Hayasaki, Masahiro Sato
    • Organizer
      45rd Annual Meeting & Exhibition of the AADR/ 40h Annual Meeting of the CADR
    • Place of Presentation
      Los Angeles (USA)
    • Year and Date
      2016-03-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒト乳歯歯髄細胞のアルカリホスファターゼ活性とOCT3/4発現はiPS細胞樹立の可否を予測する有効なマーカーである2015

    • Author(s)
      稲田絵美,佐藤正宏,齊藤一誠,窪田直子,澤味 規,村上智哉
    • Organizer
      第53回日本小児歯科学会大会
    • Place of Presentation
      広島市広島国際会議場
    • Year and Date
      2015-05-21 – 2015-05-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] piggyBac-transposon-mediated gene-delivery efficiently generates stable transfectants from HDDPCs and HDDPC-derived-iPSCs.2015

    • Author(s)
      Miki Soda, Issei Saitoh, Emi Inada, Tomoya Murakami, Yoko Iwase, Naoko Kubota, Tadashi Sawami, Yuko Matsumoto, Youichi Yamasaki, Haruaki Hayasaki, Hayato Ohshima, Masahiro Sato.
    • Organizer
      IADR/AADR/CADR 93rd General Session and Exhibition
    • Place of Presentation
      Boston, U.S.A.
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] piggyBacトランスポゾンによるヒト乳歯歯髄細胞からの遺伝子導入安定株の効率的取得2014

    • Author(s)
      稲田絵美,齊藤一誠,窪田直子,松本裕子,村上智哉,澤味規,山崎要一
    • Organizer
      第52回日本小児歯科学会大会
    • Place of Presentation
      東京品川区きゅりあん
    • Year and Date
      2014-05-16 – 2014-05-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Multipotency of juvenile human buccal epithelial cells2014

    • Author(s)
      Tomoya Murakami, Issei Saitoh, Yoko Iwase, Emi Inada, Junko Matsuyama, Kuniko Nakakura-Ohshima, Haruaki Hayasaki, Masahiro Sato
    • Organizer
      43rd Annual Meeting & Exhibition of the AADR/ 38th Annual Meeting of the CADR
    • Place of Presentation
      Charlotte, N.C., USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Choice of feeders is important for the preparation of iPS cells from primarily cultured human deciduous tooth dental pulp cells2013

    • Author(s)
      村上智哉,齊藤一誠,稲田絵美,岩瀬陽子,長谷川大子,窪田直子,松本祐子,大島邦子,岡暁子,山﨑要一,早﨑治明
    • Organizer
      第51回日本小児歯科学会大会および総会
    • Place of Presentation
      岐阜市
    • Related Report
      2013 Annual Research Report
  • [Presentation] トランスポゾンPiggyBacシステムによる複数遺伝子のブタ細胞への同時導入2013

    • Author(s)
      郡山実優,稲田絵美,齋藤一誠,三浦浩美,大塚正人,中村伸吾,桜井敬之,渡部聡,三好和睦,佐藤正宏
    • Organizer
      第106回日本繁殖生物学会大会
    • Place of Presentation
      東京都府中市
    • Related Report
      2013 Annual Research Report
  • [Remarks] 新潟大学 医歯学総合研究科 小児歯科学分野

    • URL

      http://www.dent.niigata-u.ac.jp/pedo/pedo.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 新潟大学大学院医歯学総合研究科 小児歯科学分野

    • URL

      http://www.dent.niigata-u.ac.jp/pedo/pedo.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] http://www.dent.niigata-u.ac.jp/pedo/pedo.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 新潟大学小児歯科学分野

    • URL

      http://www.pediatric-dent.net/pedo.html

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

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