| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
In this study, we used multiomics analysis-based approach to uncover the molecular basis of Porphyromonas gingivalis-Streptococcus oralis mixed-biofilm formation. Compared to the production profile in the P. gingivalis monobiofilm, production of several proteins was induced and that of some proteins was suppressed in the mixed biofilm. Similar results were obtained in mRNA level. Moreover, we constructed malate dehydrogenase-lacked P. gingivalis. The mutant showed less biofilm formation with S. oralis than the parent strain, indicating that malete dehydrogenase is important in the mixed-biofilm formation.
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