• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Comprehensive multiomics analysis of mechanism of biofilm formation by oral normal baciterlal flora and periodontopathic bacteria

Research Project

Project/Area Number 25293426
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Social dentistry
Research InstitutionOsaka University

Principal Investigator

Nagata Hideki  大阪大学, 歯学研究科(研究院), 研究員 (50260641)

Co-Investigator(Kenkyū-buntansha) 久保庭 雅恵  大阪大学, 歯学部附属病院, 講師 (00303983)
前田 和彦  大阪大学, 歯学研究科(研究院), 助教 (00346165)
関根 伸一  大阪大学, 歯学部附属病院, 助教 (70506344)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywordsバイオフィルム / 歯周病細菌 / 口腔常在菌 / マルチオミクス / トランスクリプト―プ / トランスクリプトーム
Outline of Final Research Achievements

In this study, we used multiomics analysis-based approach to uncover the molecular basis of Porphyromonas gingivalis-Streptococcus oralis mixed-biofilm formation. Compared to the production profile in the P. gingivalis monobiofilm, production of several proteins was induced and that of some proteins was suppressed in the mixed biofilm. Similar results were obtained in mRNA level. Moreover, we constructed malate dehydrogenase-lacked P. gingivalis. The mutant showed less biofilm formation with S. oralis than the parent strain, indicating that malete dehydrogenase is important in the mixed-biofilm formation.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (32 results)

All 2016 2015 2014 2013

All Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Acknowledgement Compliant: 3 results,  Open Access: 1 results) Presentation (23 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Book (1 results)

  • [Journal Article] Prediction of periodontal inflammation via metabolic profiling of saliva2016

    • Author(s)
      Kuboniwa M, Sakanaka A, Hashino E, Bamba T, Fukusaki E, Amano A
    • Journal Title

      Journal of Dental Research

      Volume: 95 Pages: 1381-1386

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distinct signatures of dental plaque metabolic byproducts dictated by periodontal inflammatory status2016

    • Author(s)
      Sakanaka A, Kuboniwa M, Hashino E, Bamba T, Fukusaki E, Amano A
    • Journal Title

      Scientific Reports

      Volume: 7

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dual lifestyle of Porphyromonas gingivalis in biofilm and gingival cells2016

    • Author(s)
      Sakanaka A, Takeuchi H, Kuboniwa M, Amano A*
    • Journal Title

      Microbial Pathogenesis

      Volume: 94 Pages: 42-47

    • DOI

      10.1016/j.micpath.2015.10.003

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Rapid identification and quantitation for oral bacteria based on short-end capillary electrophoresis2016

    • Author(s)
      Jin Chen, Yi Ni, Chenchen Liu, Yoshinori Yamaguchi, Qinmiao Chen, Shinichi Sekine, Xifang Zhu, Xiaoming Dou
    • Journal Title

      Talanta

      Volume: 160 Pages: 425-430

    • DOI

      10.1016/j.talanta.2016.07.049

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Gene analysis of multiple oral bacteria by the polymerase chain reaction coupled with capillary polymer electrophoresis2016

    • Author(s)
      Liu C, Yamaguchi Y, Sekine S, Ni Y, Zhu X, Dou X.
    • Journal Title

      J Sep Sci

      Volume: 39(5) Issue: 5 Pages: 986-992

    • DOI

      10.1002/jssc.201501087

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Antibacterial Activity of Curcumin Against Periodontopathic Bacteria.2016

    • Author(s)
      Izui S, Sekine S, Maeda K, Kuboniwa M, Takada A, Amano A, Nagata H.
    • Journal Title

      J Periodontol.

      Volume: 87(1) Issue: 1 Pages: 83-90

    • DOI

      10.1902/jop.2015.150260

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic and Transcriptional Analysis of Interaction between Oral Microbiota Porphyromonas gingivalis and Streptococcus oralis.2015

    • Author(s)
      Maeda K, Nagata H, Ojima M, Amano A
    • Journal Title

      Journal of Proteome Research

      Volume: 14 Issue: 1 Pages: 82-94

    • DOI

      10.1021/pr500848e

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] ユーカリ抽出物配合チューインガムの口臭抑制効果2013

    • Author(s)
      永田英樹
    • Journal Title

      J Japan Association on Odor Environment

      Volume: 44 Pages: 253-260

    • NAID

      130006164739

    • Related Report
      2013 Annual Research Report
  • [Presentation] Insight into the oral microbiome study2016

    • Author(s)
      Kuboniwa M
    • Organizer
      2nd Symposium 2016 of Yeongnam Branch of Korean Academy of Preventive Dentistry and Oral Health
    • Place of Presentation
      Daegu, Korea
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Porphyromonas gingivalisによる腸内細菌叢変化へのクルクミンの効果2016

    • Author(s)
      泉井秀介、関根伸一、天野敦雄
    • Organizer
      第31回日本香辛料研究会・学術講演会
    • Place of Presentation
      長浜勤労者福祉会館(滋賀県長浜市)
    • Year and Date
      2016-10-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Fusobacterium nucleatumとの栄養共生におけるStreptococcus gordoniiアルギニン・オルニチンアンチポーターArcDの機能解析2016

    • Author(s)
      坂中哲人、久保庭雅恵
    • Organizer
      大阪大学歯学会第122回例会
    • Place of Presentation
      大阪大学(大阪府吹田市)
    • Year and Date
      2016-07-14
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Salivary microbially-derived metabolic signatures are linked to periodontal inflammatory status2016

    • Author(s)
      Sakanaka A, Kuboniwa M, Hashino E, Fukusaki E, Amano A
    • Organizer
      94th IADR General Meeting
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2016-06-24
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Relationship between tongue coating and salivary metabolome2016

    • Author(s)
      Sakanaka A, Kuboniwa M, Hashino E, Amano A
    • Organizer
      12th International Conference of Asian Academy of Preventive Dentistry
    • Place of Presentation
      東京医科歯科大学(東京都文京区)
    • Year and Date
      2016-05-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Porphyromonas gingivalisベシクルによる細胞傷害に対するウコン・クルクミンの阻害効果2016

    • Author(s)
      泉井秀介、関根伸一、永田英樹、天野敦雄
    • Organizer
      第65回日本口腔衛生学会・総会
    • Place of Presentation
      東京医科歯科大学(東京都文京区)
    • Year and Date
      2016-05-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] ラマン分光散乱を用いた新規口臭測定装置の開発2016

    • Author(s)
      関根伸一、泉井秀介、永田英樹、天野敦雄
    • Organizer
      第65回日本口腔衛生学会・総会
    • Place of Presentation
      東京医科歯科大学(東京都文京区)
    • Year and Date
      2016-05-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Porpyhromonas gingivalisベシクルによる細胞傷害に対するウコン・クルクミンの阻害効果2016

    • Author(s)
      泉井秀介、関根伸一、永田英樹、天野敦雄
    • Organizer
      第65回日本口腔衛生学会・総会
    • Place of Presentation
      東京医科歯科大学(東京都文京区)
    • Year and Date
      2016-05-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] クルクミンの歯周病予防素材としての可能性について2016

    • Author(s)
      泉井秀介、関根伸一、永田英樹、天野敦雄
    • Organizer
      第5回口腔保健用機能性食品研究会・総会
    • Place of Presentation
      東北大学大学院歯学研究科(宮城県、仙台市)
    • Year and Date
      2016-01-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] クルクミンがPorphyromonas gingivalisベシクルのヒト歯肉上皮細胞への侵入に及ぼす影響2015

    • Author(s)
      泉井秀介、永田英樹、天野敦雄
    • Organizer
      第57回歯科基礎医学会学術大会
    • Place of Presentation
      朱鷺メッセ(新潟県、新潟市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] Porphyromonas gingvalisのリンゴ酸脱水素酵素がバイオフィルム形成に及ぼす影響2015

    • Author(s)
      前田和彦,永田英樹,小島美樹,天野敦雄
    • Organizer
      第64回日本口腔衛生学会・総会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-05-27 – 2015-05-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] マイクロチップPCRとキャピラリー電気泳動システムを用いた即時細菌検査システムの開発2015

    • Author(s)
      関根伸一,泉井秀介,橋野恵衣,永田英樹,天野敦雄
    • Organizer
      第64回日本口腔衛生学会・総会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-05-27 – 2015-05-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Inhibitory effects of curcumin on Inflammatory responses of gingival epithelial cells stimulated with Porphyromonas gingivalis vesicles2014

    • Author(s)
      Shusuke Izui, Shinichi Sekine, Hideki Nagata, Atsuo Amano
    • Organizer
      The 62nd Annual Meeting of Japanese Association for Dental Research
    • Place of Presentation
      KKR Hotel OSAKA
    • Year and Date
      2014-12-04 – 2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] Inhibitory effect of curcumin on Porphyromonas gingivalis infection2014

    • Author(s)
      Shusuke Izui, Shinichi Sekine, Hideki Nagata, Atsuo Amano
    • Organizer
      The 11st International Conference of Asian Academy of Preventive Dentistry
    • Place of Presentation
      Beijing, China
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 歯周ポケット内細菌叢の複雑化が脂質プロフィールに与える影響・吹田研究2014

    • Author(s)
      清原宏之,小島美樹,小野高裕,泉井秀介,関根伸一,永田英樹,天野敦雄
    • Organizer
      第63回日本口腔衛生学会・総会
    • Place of Presentation
      熊本市民会館
    • Year and Date
      2014-05-29 – 2014-05-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] クルクミンが歯周病菌の生育およびバイオフィルム形成に及ぼす影響2014

    • Author(s)
      泉井秀介,関根伸一,高田明比古,前田和彦,久保庭雅恵,永田英樹,天野敦雄
    • Organizer
      第63回日本口腔衛生学会・総会
    • Place of Presentation
      熊本市民会館
    • Year and Date
      2014-05-29 – 2014-05-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] Porpyhromonas gingivalisがStreptococcus oralisのタンパク質発現に及ぼす影響2014

    • Author(s)
      前田和彦,永田英樹,小島美樹,関根伸一,泉井秀介,天野敦雄
    • Organizer
      第63回日本口腔衛生学会・総会
    • Place of Presentation
      熊本市民会館
    • Year and Date
      2014-05-29 – 2014-05-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] 小型印刷電極を用いたP. gingivalis線毛II型株検出システムの臨床応用2013

    • Author(s)
      関根伸一、橋野恵衣、久保庭雅恵、永田英樹、天野敦雄
    • Organizer
      第62回日本口腔衛生学会・総会
    • Place of Presentation
      キッセイ文化ホール(松本)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Porphyromonas gingivalis MDHの混合バイオフィルム形成に及ぼす阻害効果2013

    • Author(s)
      前田和彦、永田英樹、小島美樹、天野敦雄
    • Organizer
      第62回日本口腔衛生学会・総会
    • Place of Presentation
      キッセイ文化ホール(松本)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 機能性食品の機能評価方法:歯周組織維持機能評価2013

    • Author(s)
      永田英樹
    • Organizer
      第62回日本口腔衛生学会・総会
    • Place of Presentation
      キッセイ文化ホール(松本)
    • Related Report
      2013 Annual Research Report
  • [Presentation] クルクミンが歯周病菌の増殖とプロテアーゼ活性に及ぼす影響2013

    • Author(s)
      泉井秀介、関根伸一、前田和彦、永田英樹、天野敦雄
    • Organizer
      第24回近畿・中国・四国口腔衛生学会総会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 口腔レンサ球菌との混合培養におけるPorphyromonas gingivalisタンパク質発現のショットガンプロテオミクス2013

    • Author(s)
      前田和彦、永田英樹、小島美樹、天野敦雄
    • Organizer
      第24回近畿・中国・四国口腔衛生学会総会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Potential candidates for preventing Porphyromonas gingivalis biofilm formation2013

    • Author(s)
      Hideki Nagata
    • Organizer
      The 2nd Japan-Thailand-Korea Joint Symposium
    • Place of Presentation
      Yonsei Yuniversity (Korea)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] Studies on Periodontal Disease2014

    • Author(s)
      Hideki Nagata
    • Total Pages
      370
    • Publisher
      Humana Press
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi