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Elucidation of relationship between the results obtained from comprehesive analysis of oral flora by using a semiconductor sequencer and health condtion

Research Project

Project/Area Number 25293428
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Social dentistry
Research InstitutionKyushu University

Principal Investigator

Yamashita Yoshihisa  九州大学, 歯学研究科(研究院), 教授 (20192403)

Co-Investigator(Kenkyū-buntansha) 江島 伸興  大分大学, 医学部, 教授 (20203630)
古田 美智子  九州大学, 歯学研究科(研究院), 助教 (20509591)
竹下 徹  九州大学, 歯学研究科(研究院), 准教授 (50546471)
清原 裕  九州大学, 医学(系)研究科(研究院), 教授 (80161602)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywords口腔フローラ / 網羅的解析 / 16S rRNA遺伝子 / 次世代シーケンサー / 分子疫学
Outline of Final Research Achievements

Clarification of a commonality and the individual difference of the Japanese oral microbiome were explored and then relationship between microbiome compostion and oral health was elucidated in this study. The salivary microbiome of dwelling population in Hisayam town was able to be classified roughly into Type I and Type II based on bacterial constitution. The subjects with Type II microbiome were younger, and with lower body mass index, lesser caries experience, and fewer smoking habit, when compared with those with Type I microbiome. These differences were significant by Poisson regression analysis. The main origin of salivary bacteria is thought to be tongue dorsum, suggesting the possibility that general health condition such as age and the degree of obesity is related to tongue coating rather than bacteria on tooth surfaces. Considering our findings, control of tongue coating is important to maintain both oral and general health.

Report

(4 results)
  • 2016 Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (35 results)

All 2016 2015 2014 2013 Other

All Journal Article (14 results) (of which Peer Reviewed: 7 results,  Open Access: 6 results,  Acknowledgement Compliant: 3 results) Presentation (20 results) (of which Invited: 2 results) Book (1 results)

  • [Journal Article] Bacterial diversity in saliva and oral health-related conditions: the Hisayama Study.2016

    • Author(s)
      Takeshita T, Kageyama S, Furuta M, Tsuboi H, Takeuchi K, Shibata Y, Shimazaki Y, Akifusa S, Ninomiya T, Kiyohara Y, Yamashita Y.
    • Journal Title

      Science Reorts

      Volume: 6 Issue: 1 Pages: 1-11

    • DOI

      10.1038/srep22164

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 口腔常在微生物叢の構成と健康との関連2016

    • Author(s)
      竹下徹、山下喜久
    • Journal Title

      日本乳酸菌学会誌

      Volume: 27 Pages: 3-9

    • NAID

      130005628246

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 誤嚥性肺炎予防における口腔ケアの有効性2015

    • Author(s)
      古田美智子、山下喜久
    • Journal Title

      臨床と研究

      Volume: 92 Pages: 1575-1580

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 口腔マイクロバイオーム2015

    • Author(s)
      山下喜久、竹下徹
    • Journal Title

      臨床と微生物

      Volume: 42 Pages: 711-715

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 口腔マイクロバイオーム評価の臨床的意義2015

    • Author(s)
      山下喜久、竹下徹
    • Journal Title

      臨床化学

      Volume: 44 Pages: 290-297

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Gender-specific associations of serum antibody to Porphyromonas gingivitis and inflammatory markers2015

    • Author(s)
      Furuta M, Shimazaki Y, Tanaka S, Takeuchi K, Shibata Y, Takeshita T, Nishimura F, Yamashita Y
    • Journal Title

      Biomedical Research International

      Volume: 2015 Pages: 897971-897971

    • DOI

      10.1155/2015/897971

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Risk factors for reduced salivary flow rate in a Japanese population: the Hisayama Study2015

    • Author(s)
      Takeuchi K, Furuta M, Takeshita T, Shibata Y, Shimazaki Y, Akifusa S, Ninomiya T, Kiyohara Y, Yamashita Y
    • Journal Title

      Biomedical Research International

      Volume: 2015 Pages: 381821-381821

    • DOI

      10.1155/2015/381821

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dental plaque development on a hydroxyapatite disk in young adults observed by using a barcoded pyrosequencing approach2015

    • Author(s)
      Takeshita T, Yasui M, Shibata Y, Furuta M, Saeki Y, Eshima N, Yamashita Y
    • Journal Title

      Sci Rep

      Volume: 5 Issue: 1 Pages: 8136-8136

    • DOI

      10.1038/srep08136

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Distinct composition of the oral indigenous microbiota in South Korean and Japanese adults2014

    • Author(s)
      Takeshita T, Matsuo K, Furuta M, Shibata Y, Fukami K, Shimazaki Y, Akifusa S, Han DH, Kim HD, Yokoyama T, Ninomiya T, Kiyohara Y, Yamashita Y
    • Journal Title

      Sci Rep

      Volume: 4 Issue: 1 Pages: 6990-6990

    • DOI

      10.1038/srep06990

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of the microbiota in carious dentin lesions using 16S rRNA gene sequencing2014

    • Author(s)
      Obata J, Takeshita T, Shibata Y, Yamanaka W, Unemori M, Akamine A, Yamashita Y
    • Journal Title

      PLoS One

      Volume: 9 Issue: 8 Pages: e103712-e103712

    • DOI

      10.1371/journal.pone.0103712

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Supervised machine learning-based prediction of oral malodor based on the microbiota in saliva samples2014

    • Author(s)
      Nakano Y, Takeshita T, Kamio N, Shiota S, Shibata Y, Suzuki N, Yoneda M, Hirofuji T, Yamashita Y
    • Journal Title

      Artif Intell Med

      Volume: 60 Pages: 97-101

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 口腔マイクロバイオーム解析の歯科医学における臨床的意義2014

    • Author(s)
      山下喜久,竹下徹
    • Journal Title

      実験医学

      Volume: 32 Pages: 733-738

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
  • [Journal Article] 口腔疾患とマイクロバイオーム2013

    • Author(s)
      山下喜久、竹下徹
    • Journal Title

      医学のあゆみ

      Volume: 246 Pages: 1089-1093

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 口腔のマイクロバイオーム2013

    • Author(s)
      竹下徹、山下喜久
    • Journal Title

      細胞工学

      Volume: 32 Pages: 1125-1128

    • Related Report
      2013 Annual Research Report
  • [Presentation] 歯科からみた呼吸器感染症2016

    • Author(s)
      山下喜久
    • Organizer
      第46回日本嫌気性菌感染症学会
    • Place of Presentation
      長崎大学医学部良順会館、長崎市
    • Year and Date
      2016-03-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] Ion PGMシステムを用いた大規模メタ16S解析による健康に関わる口腔マイクロバイオームの探索2015

    • Author(s)
      竹下徹
    • Organizer
      Applied Biosystems /Ion Torrent ユーザーグループミーティング2015
    • Place of Presentation
      品川シーズンテラスカンファレンス、東京
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 疾患および健康に関連する口腔常在マイクロバイオームの探索2015

    • Author(s)
      竹下徹
    • Organizer
      日本乳酸菌学会秋季セミナー
    • Place of Presentation
      昭和女子大学大講堂、東京
    • Year and Date
      2015-11-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] ADLの低下した無歯顎高齢者の口腔マイクロバイオーム2015

    • Author(s)
      影山伸哉、竹内研時、泉繭依、我那覇生純、竹下徹、柴田幸江、古田美智子、山下嘉久
    • Organizer
      第9回細菌学若手コロッセウム
    • Place of Presentation
      レインボー桜島、鹿児島市
    • Year and Date
      2015-11-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] Salivary microbiome and environmental conditions in the oral cavity2015

    • Author(s)
      Takeshita T, Yamashita Y
    • Organizer
      第63回国際歯科研究学会JADR
    • Place of Presentation
      福岡国際会議場、福岡市
    • Year and Date
      2015-10-31
    • Related Report
      2015 Annual Research Report
  • [Presentation] A Molecular epidemiology study exploring oral microbiome structure associated with human health2015

    • Author(s)
      Takeshita T, Yamashita Y
    • Organizer
      歯科基礎医学会サテライトシンポジウム
    • Place of Presentation
      朱鷺メッセ、新潟市
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 歯の有無が口腔常在細菌叢に与える影響2015

    • Author(s)
      影山伸哉、竹内研時、泉繭依、我那覇生純、竹下徹、柴田幸江、古田美智子、山下嘉久
    • Organizer
      第37回九州口腔衛生学会・総会
    • Place of Presentation
      長崎県歯科医師会館、長崎市
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] 生活機能の低下が口腔常在細菌叢に与える影響2015

    • Author(s)
      影山伸哉、竹内研時、泉繭依、我那覇生純、竹下徹、柴田幸江、古田美智子、山下嘉久
    • Organizer
      第64回日本口腔衛生学会・総会
    • Place of Presentation
      つくば国際会議場、つくば市
    • Year and Date
      2015-05-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Bacterial diversity and community types in saliva and oral environment2015

    • Author(s)
      Toru Takeshita, Michiko Furuta, Hidenori Tsuboi, Shinya Kageyama, Yoshihiro Shimazaki, Toshiharu Ninomiya, Yutaka Kiyohara, Yoshihisa Yamashita
    • Organizer
      5th International Human Microbiome Congress
    • Place of Presentation
      Luxembourg, Luxembourg
    • Year and Date
      2015-03-31 – 2015-04-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] Myrna Z, Takeshita T, Furuta M, Maeda H, Akamine A, Ninomiya T, Kiyohara Y, Yamashita Y2015

    • Author(s)
      Oral mycobiome in Japanese elderly adults
    • Organizer
      93rd General Session & Exhibition of the IADR
    • Place of Presentation
      Boston, USA
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] Relationship among carious dentin, dental plaque and salivary microbiota2015

    • Author(s)
      Obata J, Takeshita T, Shibata Y, Unemori M, Akamine A, Yamashita Y
    • Organizer
      93rd General Session & Exhibition of the IADR
    • Place of Presentation
      Boston, USA
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] 歯垢と舌苔が唾液の細菌構成に与える影響2015

    • Author(s)
      影山伸哉、竹下徹、柴田幸江、山下喜久
    • Organizer
      第9回日本ゲノム微生物学会
    • Place of Presentation
      神戸市
    • Year and Date
      2015-03-06 – 2015-03-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] Tongue microbiome in ddentulous infants and dentate children2014

    • Author(s)
      Yamashita Y, Takeshita T
    • Organizer
      2nd World Congress on Targeting Microbiota
    • Place of Presentation
      institut Pasteur, Paris, France
    • Year and Date
      2014-10-15 – 2014-10-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of oral indigenous microbiota in early childhood2014

    • Author(s)
      Takeshita T, Obata J, Tsuboi H, Yamashita Y
    • Organizer
      15th International Symposium on Microbial Ecology
    • Place of Presentation
      ソウル市、大韓民国
    • Year and Date
      2014-08-24 – 2014-08-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 口腔マイクロバイオームの構成パターンと口腔の健康状態との関連2014

    • Author(s)
      竹下徹、坪井秀憲、古田美智子、嶋崎義浩、柴田幸江、清原裕、山下喜久
    • Organizer
      第18回腸内細菌学会
    • Place of Presentation
      東京大学、東京
    • Year and Date
      2014-06-11 – 2014-06-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Comparison of salivary microbiota between Japanese and Koreans

    • Author(s)
      Y. Yamashita, T. Takeshita, K. Matsuo, D-H Han, H-D Kim
    • Organizer
      Cell Symposia Microbiome and Host Health
    • Place of Presentation
      Lisbon, Portugal
    • Related Report
      2013 Annual Research Report
  • [Presentation] 口腔の健康な成人の口腔常在フローラの構成

    • Author(s)
      竹下徹、深水薫、嶋崎義浩、古田美智子、柴田幸江、清原裕、山下喜久
    • Organizer
      第17回腸内細菌学会
    • Place of Presentation
      東京都港区白金
    • Related Report
      2013 Annual Research Report
  • [Presentation] 久山町コホート研究における口腔と全身の健康の関連性解明へのアプローチ

    • Author(s)
      山下喜久
    • Organizer
      第72回日本公衆衛生学会
    • Place of Presentation
      三重県津市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 分子疫学的手法を用いた宿主の健康に関わる口腔マイクロバイオームの探索

    • Author(s)
      竹下徹
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京江戸川区船堀
    • Related Report
      2013 Annual Research Report
  • [Presentation] 口腔マイクロバイオーム解析の臨床的意義

    • Author(s)
      山下喜久
    • Organizer
      Roche Scientific Symposium 2013
    • Place of Presentation
      東京ステーションカンファレンス
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] ヒトマイクロバイオーム研究最前線2016

    • Author(s)
      山下喜久、竹下徹
    • Total Pages
      472
    • Publisher
      株式会社エヌ・ティー・エス
    • Related Report
      2015 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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