Study on the lymphatic edema and wound, honey and burn, estrogen and wound for the nursing care by using animal

• Project/Area Number: 25293430
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Fundamental nursing
• Research Institution: Kanazawa University
• Principal Investigator: Nakatani Toshio 金沢大学, 保健学系, 教授 (60198124)
• Co-Investigator(Kenkyū-buntansha): SUGAMA Junko 金沢大学, 保健学系, 教授 (00203307) ; OKUWA Mayumi 金沢大学, 保健学系, 教授 (30303291) ; NISHIZAWA Tomoe 新潟大学, 医歯学系, 准教授 (50579597) ; DAI Misako 金沢大学, 保健学系, 助教 (50614864)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
• Keywords: 皮膚創傷 / 看護ケア / リンパ浮腫 / 蜂蜜 / 火傷 / エストロゲン / 常温プラズマ / 脂肪細胞 / 筋注 / 皮膚創傷治癒 / 低タンパク質 / リンパ管 / 熱傷 / 低栄養 / プラズマ

Outline of Final Research Achievements

A mouse model of lymphedema after inguinal lymphadenectomy with a skin would at the same site was created to examine the influences of the condition. Honey, and estrogen as a female hormone on would healing were examined. The following findings were obtained: the influence of lymphedema on the wound healing remained unclear, as lymphedema did not persist; the effect of honey on the third-degree burn was poor; estrogen positively affected wound healing in elderly and malnourished mice; and a plasma jet generated by applying a high voltage to argon gas was also suggested to promote would healing.

Research Products

• [Journal Article] A simple technique to improve contractile effect of cold plasma jet on acute wound of mouse by dropping water. (2015)
  • Author(s): Nasruddin, Yukari Nakajima, Kanae Mukai, Emi Komatsu, Heni Setyowati Esti Rahayu, Muhammad Nur, Tatsuo Ishijima, Hiroshi Enomoto, Yoshihiko Uesugi, Junko Sugama, Toshio Nakatani
  • Journal Title: Plasma Processes and Polymers Volume: - Issue: 10 Pages: 1128-1138
  • DOI: 10.1002/ppap.201400236
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evaluation of the effects of a combination of Japanese honey and hydrocolloid dressing on cutaneous wound healing in male mice (2015)
  • Author(s): Kanae Mukai, Miki Koike, Saki Nakamura, Fumika Katagiri, Saki Nojiri, Yuki Yamada, Eri Miyajima, Mayuko Matsumoto, Emi Komatsu, Yukari Nakajima, Tamae Urai, Naoko Murakado, Toshio Nakatani
  • Journal Title: Evidence-Based Complementary and Alternative Medicine Volume: 2015 Pages: 1-9
  • DOI: 10.1155/2015/910605
  • NAID: 120005613975
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Anatomical investigation of position of crossing of superficial branch of radial nerve and cephalic vein and its frequency (2015)
  • Author(s): Kanae Mukai, Emi Komatsu, Tamae Urai, Yukari Nakajima, Kimi Asano, Noriyuki Ozaki, Toshio Nakatani
  • Journal Title: Structure and Function（形態・機能） Volume: 14 Pages: 3-11
  • NAID: 130005100065
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 未経験者による三角筋筋肉内注射の位置決め器具の使用に関する正確性と簡便性の生体での検討 (2015)
  • Author(s): 向井 加奈恵，小松 恵美，中島 由加里，浦井 珠恵，浅野 きみ，中谷 壽男
  • Journal Title: 形態・機能 Volume: 14 Pages: 75-83
  • NAID: 130005144368
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 17β-estradiol administration promotes delayed cutaneous wound healing in 40-week ovariectomized female mice. (2015)
  • Author(s): Kanae Mukai, Yukari Nakajima, Tamae Urai, Emi Komatsu, Nasruddin, Junko Sugama, Toshio Nakatani
  • Journal Title: International Wound Journal Volume: - Issue: 5 Pages: 636-644
  • DOI: 10.1111/iwj.12336
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cold plasma on full-thickness cutaneous wound accelerates healing through promoting inflammation, re-epithelialization and wound contraction (2014)
  • Author(s): Nasruddin, Y. Nakajima, K. Mukai, H. Setyowati Esti Rahayu, Muhammad Nur, T. Ishijima, H. Enomoto, Y. Uesugi, J. Sugama, T. Nakatani
  • Journal Title: Clinical Plasma Medicine Volume: 2 Issue: 1 Pages: 1-5
  • DOI: 10.1016/j.cpme.2014.01.001
  • NAID: 120005411692
  • Peer Reviewed / Open Access
• [Journal Article] The effect of 17ß-estradiol administration on cutaneous wound healing in 24-week ovariectomized female mice. (2014)
  • Author(s): Kanae Mukai, Yukari Nakajima, Tamae Urai, Emi Komatsu, Kana Takata, Yuriko Miyasaka, Nasruddin, Junko Sugama, Toshio Nakatani
  • Journal Title: Journal of Hormones Volume: 2014 Pages: 1-8
  • DOI: 10.1155/2014/234632
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Comparison of the cutaneous wound healing of ovariectomized mouse at 12 weeks with that of SHAM and estrogen-administered mice (2014)
  • Author(s): Kanae Mukai, Yuriko Miyasaka, Kana Takata, Tamae Urai, Yukari Nakajima, Emi Komatsu, Junko Sugama, Toshio Nakatani,
  • Journal Title: Journal of Hormones Volume: 2014 Pages: 1-6
  • DOI: 10.1155/2014/484258
  • NAID: 120005456205
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ご遺体での三角筋筋注部位の位置決め器具の有用性と決定した筋注部位の安全性の検討． (2014)
  • Author(s): 小松 恵美，向井 加奈恵，中島 由加里，尾崎 紀之，中谷 壽男
  • Journal Title: 形態・機能 Volume: 13 Pages: 17-24
  • NAID: 130005068662
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The effect of 17β-estradiol on cutaneous wound healing in protein-malnourished ovariectomized female mouse model (2014)
  • Author(s): Kanae Mukai, Emi Komatsu, Yukari Nakajima, Tamae Urai, Nasruddin, Junko Sugama, Toshio Nakatani
  • Journal Title: PLoS ONE Volume: 9 Issue: 12 Pages: 1-20
  • DOI: 10.1371/journal.pone.0115564
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Is estrogen effective for full-thickness cutaneous wound healing in young male mice? (2013)
  • Author(s): Y. Nakajima, Y. Eno, M. Hirata, S. Kobori, A. Sugiura, M. Takeuchi, M Taniguchi, M. Tanisaki, A. Hayashi, K. HikishimaN. Matsuo, N. Wada, K. Mukai, N. Murakado, M. Okuwa, T. Nakatani
  • Journal Title: Wounds Volume: 25 Pages: 278-286
  • NAID: 120005342818
  • Peer Reviewed
• [Journal Article] Evaluation of the effects of honey on acute-phase deep burn wounds (2013)
  • Author(s): Yukari Nakajima, Kanae Mukai, Nasruddin, Emi Komatsu, Terumi Iuchi, Yukie Kitayama, Junko Sugama, Toshio Nakatani
  • Journal Title: Evidence-Based Complementary and Alternative Medicine Volume: 2013 Pages: 1-20
  • DOI: 10.1155/2013/784959
  • NAID: 120005347250
  • Peer Reviewed
• [Journal Article] Effects of three types of Japanese honey on full-thickness wound in mice (2012)
  • Author(s): ukari Nakajima, Kanae Mukai, Yukie Kitayama, Naoko Murakado, Mayumi Okuwa,Toshio Nakatani
  • Journal Title: Evidence-Based Complementary and Alternative Medicine Volume: 2013 Pages: 11-11
  • DOI: 10.1155/2013/504537
  • NAID: 120005253835
  • Peer Reviewed
• [Presentation] Evaluation of the effect of estradiol benzoate on cutaneous wound healing in ovariectomized female mice –Focus on the inflammatory response- (2016)
  • Author(s): Kanae Mukai, Tamae Urai, Kimi Asano, Yukari Nakajima, Toshio Nakatani
  • Organizer: WUWHS2016
  • Place of Presentation: イタリア フィレンツェ
  • Year and Date: 2016-09-25
  • Int'l Joint Research
• [Presentation] Effects of honeydew honey on wound healing (2016)
  • Author(s): Yukari Nakajima, Kanae Mukai, Tamae Urai, Kimi Asano, Megumi Ohta, Ikuko Kato, Azusa Kawaguchi, Yui Kinoshita, Yuri Kumagai, Ayaka Sakashita, Takuto Sawazaki,, Asuka Yamazaki, Toshio Nakatani
  • Organizer: WUWHS2016
  • Place of Presentation: イタリア フィレンツェ
  • Year and Date: 2016-09-25
  • Int'l Joint Research
• [Presentation] 鼡径リンパ節を郭清した下腿に作製した創傷は治癒が遅延するか (2015)
  • Author(s): 中谷壽男，小松恵美，浅野きみ，向井加奈恵，浦井珠恵，中島由加里
  • Organizer: 第45回日本創傷治癒学会
  • Place of Presentation: 東京都千代田区 JRタワーホール＆カンファレンス
  • Year and Date: 2015-11-30
• [Presentation] Estradiol Benzoateの創部塗布による皮膚創傷治癒への効果―肉眼的検討― (2015)
  • Author(s): 向井加奈恵，浦井珠恵，浅野きみ，中島由加里，須釜淳子，中谷壽男
  • Organizer: 第45回日本創傷治癒学会
  • Place of Presentation: 東京都千代田区 JRタワーホール＆カンファレンス
  • Year and Date: 2015-11-30
• [Presentation] 三角筋筋肉内注射位置決め器具の使用未経験者における測定部位のずれの検討 (2015)
  • Author(s): 浦井珠恵、向井加奈恵、小松恵美、中島由加里、浅野きみ、中谷壽男
  • Organizer: コ・メディカル形態機能学会 第14回学術集会
  • Place of Presentation: 埼玉県入間郡埼玉医科大学川角キャンパス
  • Year and Date: 2015-09-26
• [Presentation] リンパ節切除とリンパ管切断による皮膚創傷治癒への影響 (2015)
  • Author(s): 小松恵美，向井加奈恵，浦井珠恵，浅野きみ，中島由加里，中谷壽男
  • Organizer: 第17回日本褥瘡学会学術集会
  • Place of Presentation: 宮城県仙台市仙台国際センター
  • Year and Date: 2015-08-28
• [Presentation] Controlling the effect of cold plasma treatment on mouse skin wound by modifying its surface using water (2015)
  • Author(s): Nasruddin,Yukari Nakajima, Kanae Mukai, Emi Komatsu, Heni Setyowati Esti Rahayu, Muhammad Nur, Tatsuo Ishijima, Hiroshi Enomoto, Yoshihiko Uesugi, Junko Sugama, Toshio Nakatani
  • Organizer: ISPLASMA 2015 (8th International Conference on Plasma-Nano technology and Science) i
  • Place of Presentation: 名古屋大学（（愛知県名古屋市）
  • Year and Date: 2015-03-26 – 2015-03-31
• [Presentation] 看護学教育での解剖生理学の教育の方法 Teaching anatomy and physiology in nursing education (2015)
  • Author(s): 中谷 壽男
  • Organizer: Symposium 30 コメディカル解剖・生理学教育に必要と思われる教授内容と視点 第120回日本解剖学会・全国学術集会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-03-21 – 2015-03-23
  • Invited
• [Presentation] Cutaneous tissue repair following atmospheric plasma jet treatment (2015)
  • Author(s): Nasruddin,Yukari Nakajima, Kanae Mukai, Emi Komatsu, Heni Setyowati Esti Rahayu, Muhammad Nur, Tatsuo Ishijima, Hiroshi Enomoto, Yoshihiko Uesugi, Junko Sugama, Toshio Nakatani
  • Organizer: 2nd International Workshop on Plasma for Cancer Therapy (IWPCT)
  • Place of Presentation: 名古屋大学東山キャンパス（愛知県名古屋市）
  • Year and Date: 2015-03-16 – 2015-03-17
• [Presentation] Influence of obesity on cutaneous wound healing in an obese mouse model fed a high-fat diet (2015)
  • Author(s): Tamae Urai, Kanae Mukai, Tatsuhiko Matsushita, Emi Komatsu, Yukari Nakajima, Junko Sugama, Toshio Nakatani
  • Organizer: 18th East Asian Forum Of Nursing Scholars（EAFONS）
  • Place of Presentation: NTUH International Convention Center (Taiwan)
  • Year and Date: 2015-02-05 – 2015-02-06
• [Presentation] 3%蛋白質含有食飼料を摂取した卵巣切除マウスの皮膚創傷への17β-estradiol投与の効果 (2014)
  • Author(s): 向井加奈恵、小松恵美、中島由加里、浦井珠恵、須釜淳子、中谷壽男
  • Organizer: 第44回日本創傷治癒学会
  • Place of Presentation: ホテルメトロポリタン仙台（宮城県仙台市）
  • Year and Date: 2014-12-02 – 2014-12-03
• [Presentation] 三角筋筋肉注射の際に腋窩神経の走行を推測する方法の正確性と簡便性の検討 (2014)
  • Author(s): 小松恵美，向井加奈恵，中島由加里，浦井珠恵，中谷壽男
  • Organizer: 第13回日本看護技術学会学術集会
  • Place of Presentation: 京都テルサ（京都府京都市）
  • Year and Date: 2014-11-22 – 2014-11-24
• [Presentation] 橈骨神経浅枝が皮下へ出現する位置及び橈骨神経浅枝と橈側皮静脈の交叉の位置と頻度に関する解剖学的調査 (2014)
  • Author(s): 向井加奈恵、小松恵美、浦井珠恵、中島由加里、尾崎紀之、中谷壽男
  • Organizer: 日本解剖学会第74回中部支部学術集会
  • Place of Presentation: 金沢大学宝町キャンパス（石川県金沢市）
  • Year and Date: 2014-10-11 – 2014-10-12
• [Presentation] ご遺体を用いた橈側皮静脈と橈骨神経浅枝の交叉の位置と頻度の調査 (2014)
  • Author(s): 向井加奈恵、小松恵美、浦井珠恵、中島由加里、尾崎紀之、中谷壽男
  • Organizer: 第13回コ・メディカル形態機能学会
  • Place of Presentation: 北九州国際会議場（福岡県北九州市）
  • Year and Date: 2014-09-30
• [Presentation] 創傷治癒過程の炎症期においてマウスの皮膚全層欠損創へ日本産蜂蜜を使用することによる効果の検討 (2014)
  • Author(s): 小松恵美，中島由加里，向井加奈恵，大桑麻由美，中谷壽男
  • Organizer: 第16回日本褥瘡学会学術集会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2014-08-29 – 2014-08-30
• [Presentation] マウスの皮膚全層欠損創への日本産蜂蜜とハイドロコロイド被覆材併用による効果の検証 (2014)
  • Author(s): 向井加奈恵，小松恵美，中島由加里，中谷壽男
  • Organizer: 第16回日本褥瘡学会学術集会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2014-08-29 – 2014-08-30
• [Presentation] Improving the Healing Effect of Cold Plasma Treatmenton Wound using Water Adding (2014)
  • Author(s): Nasruddin, Emi Komatsu, Kanae Mukai, Yukari Nakajima, Heni SetyowatiEsti Rahayu, Muhammad Nur, Tatsuo Ishijima, Hiroshi Enomoto, Yoshihiko Uesugi, Junko Sugama, Toshio Nakatani
  • Organizer: 8th International Conference on Reactive Plasmas/ 31st Symposium on Plasma Processing
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] 17β-estradiol promotes cutaneous wound healing in 24-week ovariectomized female mice by modulating wound inflammation and promoting wound contraction (2013)
  • Author(s): Kanae Mukai, Yukari Nakajima, Emi Komatsu, Junko Sugama, Toshio Nakatani
  • Organizer: XXIII International Symposium on Morphological Sciences
  • Place of Presentation: 朱鷺メッセ新潟コンベンションセンター（新潟県）
• [Presentation] Study of the effects of combining Japanese honey and a hydrocolloid dressing on skin wound healing (2013)
  • Author(s): Emi Komatsu, M Hattori, M Yamanishi, M Hutakuchi, K Kanzaka, Y Uno, S Yamazaki, S Kato, T Yamamoto, Y Nakajima, K Mukai, N Murakado, M Ookuwa, T Nakatani
  • Organizer: XXIII International Symposium on Morphological Sciences
  • Place of Presentation: 朱鷺メッセ新潟コンベンションセンター（新潟県）
• [Presentation] 急性期三度熱傷創における蜂蜜の抗炎症作用の効果 (2013)
  • Author(s): 中島由加里，向井加奈恵、小松恵美，北山幸枝，須釜淳子，中谷壽男
  • Organizer: 第43回日本創傷治癒学会
  • Place of Presentation: 別府湾ロイヤルホテル（大分県）
• [Presentation] 蛋白質栄養不良卵巣切除マウス皮膚創傷への 17β エストラジオール経皮投与の効果―肉眼 的所見による検証- (2013)
  • Author(s): 向井 加奈恵，小松 恵美，中島 由加里，須釜 淳子，中谷 壽男
  • Organizer: 第43回日本創傷治癒学会
  • Place of Presentation: 別府湾ロイヤルホテル（大分県）
• [Presentation] 皮膚創傷治癒過程に日本産蜂蜜とハイドロコロイド被覆材を併用することによる効果の検討 (2013)
  • Author(s): 小松恵美，宇野由花、加藤静香，服部真弓，二口美佐子，山崎静，山西未紗，勘坂佳世，山本友美，中島由加里，向井加奈恵，村角直子，大桑麻由美，中谷壽男
  • Organizer: 第43回日本創傷治癒学会
  • Place of Presentation: 別府湾ロイヤルホテル（大分県）
• [Remarks] 金沢大学医薬保健研究域保健学系・金沢大学医薬保健総合研究域保健学専攻 創傷看護技術学分野
  • URL: http://wmn.w3.kanazawa-u.ac.jp
• [Remarks]
  • URL: http://mhs3.mp.kanazawa-u.ac.jp
• [Remarks]
  • URL: http://wmn.w3.kanazawa-u.ac.jp
• [Remarks] 金沢大学 看護科学領域 創傷看護技術学グループ
  • URL: http://square.umin.ac.jp/sugama/
• [Remarks] 金沢大学学術情報リポジトリKURA
  • URL: http://dspace.lib.kanazawa-u.ac.jp/dspace/