Euro-Japan collaborative field work for the understanding of symbiotic evolution of marine microbes

• Project/Area Number: 25303008
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Biomolecular chemistry
• Research Institution: Waseda University
• Principal Investigator: Nakao Yoichi 早稲田大学, 理工学術院, 教授 (60282696)
• Co-Investigator(Kenkyū-buntansha): YAMASHITA Jun 京都大学, iPS細胞研究所, 教授 (50335288)
• Co-Investigator(Renkei-kenkyūsha): KIMURA Hiroshi 東京工業大学, 大学院生命理工学研究科, 教授 (30241392) ; IKURA Tsuyoshi 京都大学, 放射線生物研究センター, 准教授 (70335686)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000); Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: 海洋天然物化学 / 共生微生物 / エピジェネティクス / 海綿 / 生理活性物質 / メタボローム解析 / メタゲノム解析 / 国際研究者交流

Outline of Final Research Achievements

We have compared the metabolome analysis of marine sponge by LC-MS/MS. As the results, we have found the difference between the matabolite profiles of two closely related sponge samples collected in Italy and Japan. Also, we could isolate highly novel microorganisms from the sponge of genus Theonella by means of newly developed Diffusion Chamber method. Through this project, a sound base for the collaborative activity between Italy-Japan marine natural products chemistry has been established.

Research Products

• [Int'l Joint Research] ジェノバ大学/ナポリ大学(イタリア)
• [Journal Article] A genetically encoded FRET probe to detect intranucleosomal histone H3K9 or H3K14 acetylation using BRD4, a BET family member (2016)
  • Author(s): Nakaoka, S., Sasaki, K., Ito, A., Nakao, Y., and Yoshida, M.
  • Journal Title: ACS Chem. Biol. Volume: 11 Issue: 3 Pages: 729-733
  • DOI: 10.1021/cb501046t
  • Peer Reviewed
• [Journal Article] An epigenetic regulatory element of the Nodal gene in the mouse and human genomes (2015)
  • Author(s): Daisuke Arai, Koji Hayakawa, Jun Ohgane, Mitsuko Hirosawa, Yoichi Nakao, Satoshi Tanaka, Kunio Shiota
  • Journal Title: Mechanisms of Development Volume: 136 Pages: 143-154
  • DOI: 10.1016/j.mod.2014.12.003
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cinanthrenol A, an Estrogenic Steroid Containing Phenanthrene (2014)
  • Author(s): Koshi Machida, Takahiro Abe,Daisuke Arai, Mayumi Okamoto, Isao Shimizu, Nicole J. de Voogd,Nobuhiro Fusetani, Yoichi Nakao
  • Journal Title: Org. Lett. Volume: 16 Issue: 6 Pages: 1539-1541
  • DOI: 10.1021/ol5000023
  • Peer Reviewed
• [Journal Article] Insights on pregnane-X-receptor modulation. Natural and (2014)
  • Author(s): Valentina Sepe, Claudio D’Amore, Raffella Ummarino, Barbara Renga, Maria Valeria D’Auria, Ettore Novellino, Annamaria Sinisi, Orazio Taglialatela-Scafati, Yoichi Nakao, Vittorio Limongelli, Angela Zampella, Stefano Fiorucci
  • Journal Title: Eur. J. Med. Chem. Volume: 73 Pages: 126-134
  • Peer Reviewed
• [Presentation] 海洋シアノバクテリアからの新規環状ペプチドの単離と構造決定 (2016)
  • Author(s): 中村 文彬、前島 寛、中尾 洋一
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 京都
  • Year and Date: 2016-03-26
• [Presentation] 熊本県産タツナミガイDolabella auricularia から得られた新規ハロゲン化ジテルペンの構造 (2016)
  • Author(s): 町田 光史、中尾 洋一
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 京都
  • Year and Date: 2016-03-24
• [Presentation] New approach to study histone modification changes controlling biological phenomena by small molecules (2015)
  • Author(s): Nakao, Y.; Arai, D.; Kina, Y.; Kataoka, R.; Hayashi-Takanaka, Y.; Kimura, H.
  • Organizer: 2015環太平洋国際化学会議（PACIFICHEM2015）
  • Place of Presentation: 米国ハワイ州
  • Year and Date: 2015-12-17
  • Int'l Joint Research
• [Presentation] Isolation and structure elucidation of an active compound affecting histone modification from the marine sponge (2015)
  • Author(s): Machida, K.; Ishibashi, N.; Kataoka, K.; Arai, D.; Hayashi-Takanaka, Y.; Kimura, H.; Fusetani, N.; Nakao, Y.
  • Organizer: 2015環太平洋国際化学会議（PACIFICHEM2015）
  • Place of Presentation: 米国ハワイ州
  • Year and Date: 2015-12-16
  • Int'l Joint Research
• [Presentation] 海洋毒・海洋生理活性物質の探索研究へのMSの応用 (2015)
  • Author(s): 中尾洋一
  • Organizer: 第63回 質量分析総合討論会
  • Place of Presentation: つくば
  • Year and Date: 2015-06-17
  • Invited
• [Presentation] Chemical biology of cell differentiation using chemical probes derived from marine natural products (2014)
  • Author(s): Otsuka, S.; Iwata, T.; Tsubokura, K.; Arai, D.; Fukase, K.; Tanaka, K.; Nakao, Y.
  • Organizer: The International Symposium on Natural Products Chemistry and Chemical Biology 2014
  • Place of Presentation: 中国杭州
  • Year and Date: 2014-11-24
  • Invited
• [Presentation] Search for natural products controlling epigenetics (2014)
  • Author(s): Nakao, Y.
  • Organizer: Sino-Japan Workshop on Chemical Biology
  • Place of Presentation: 中国北京
  • Year and Date: 2014-10-11
  • Invited
• [Presentation] 新規天然化合物shinanthrene Aとそのエストロゲン活性 (2013)
  • Author(s): 5. 町田光史、岡本真由美、清水功雄、新井大祐、伏谷伸宏、中尾洋一
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] H3K9アセチル化誘導活性を有する海洋天然化合物の探索 (2013)
  • Author(s): 10. 神平梨絵、喜納惟斗、林陽子、木村宏、新井大祐、中尾洋一
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Patent(Industrial Property Rights)] 白血病幹細胞のニッチ形成抑制活性を有する海洋生物由来の化合物及び組成物 (2016)
  • Inventor(s): 中尾洋一、浅野茂隆、新井大祐、加勢友香梨、下本哲平
  • Industrial Property Rights Holder: 早稲田大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-004523
  • Filing Date: 2016-01-13