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A Landscape Genetic Study of Dengue Vector Mosquitoes in The Philippines: Dispersal Pattern and Infection Route

Research Project

Project/Area Number 25303020
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section海外学術
Research Field Civil and environmental engineering
Research InstitutionEhime University

Principal Investigator

Watanabe Kozo  愛媛大学, 理工学研究科, 准教授 (80634435)

Co-Investigator(Kenkyū-buntansha) OMURA Tatsuo  東北大学, 未来科学技術共同研究センター, 教授 (30111248)
WATANABE Toru  山形大学, 農学部, 教授 (10302192)
Co-Investigator(Renkei-kenkyūsha) OSHITANI Hitoshi  東北大学, 医学研究科, 教授 (80419994)
SAITO Mariko  東北大学, 医学研究科, 助教 (80404234)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywordsデング熱 / ベクター感染症 / 集団遺伝 / 蚊 / フィリピン / 感染症 / GIS解析 / 生態学 / 遺伝子 / 国際研究者交流 / 生態疫学 / 土木環境システム / 国際貢献
Outline of Final Research Achievements

We assessed the spatial population genetic structure of dengue vector mosquito Aedes aegypti in Metro Manila and Tarlac city in the Philippines. The analysis inferred a strong dispersal activity of Ae aegypti based on the observed low genetic differentiation among populations. However, fine scale population structuring was also detected based on the generated dendrogram and the identification of 3 genetic populations. Based on auto-correlation among local populations, their flight distance was estimated < 5km. In addition, we also developed a GIS-based prediction model of local dengue risk focusing on flood intensity and land use classification in all 604 administrative district in Metro Manila. Using these data, we constructed a regression tree model that describes the spatial variation of dengue infection rate of the administrative section. The model was estimated with high accuracy, and flooding has also been shown that a high contribution as a risk factor.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (37 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (3 results) Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Acknowledgement Compliant: 4 results,  Open Access: 1 results) Presentation (23 results) (of which Int'l Joint Research: 6 results,  Invited: 2 results) Funded Workshop (2 results)

  • [Int'l Joint Research] デラサール大学/熱帯医学研究所(フィリピン)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] パジャジャラン大学(インドネシア)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] ライプニッツ淡水生態学・内水漁業研究所(ドイツ)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 水文モデルと底生動物の生息場モデルを用いた河川健全度パターンの評価2016

    • Author(s)
      糠澤桂,風間聡,渡辺幸三
    • Journal Title

      土木学会論文集B1(水工学)

      Volume: Vol.72, No.4

    • NAID

      130005312010

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Spatial Analysis of Wing Geometry in Dengue Vector Mosquito, Aedes aegypti (L.) (Diptera; Culicidae) Populations in Metropolitan Manila, Philippines2016

    • Author(s)
      Thaddeus M. Carvajal, Lara Fides T. Hernandez, Howell T. Ho, Menard G. Cuenca, Bianca Marie C. Orantia, Camille R. Estrada, Divina M. Amalin, and Kozo Watanabe
    • Journal Title

      Journal of Vector Borne Diseases

      Volume: accepted

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] ダムおよび瀬切れによる河川分断化がエルモンヒラタカゲロウの地域間交流に及ぼす影響の遺伝的評価2015

    • Author(s)
      八重樫咲子,不破直人,山崎久美子,三宅洋,渡辺幸三
    • Journal Title

      土木学会論文集G(環境)

      Volume: Vol.71, No.7

    • NAID

      130005155790

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A hydrothermal simulation approach to modelling spatial patterns of adaptive genetic variation in four stream insects2015

    • Author(s)
      Nukazawa K., Kazama S., Watanabe K.
    • Journal Title

      Journal of Biogeography

      Volume: 42 Issue: 1 Pages: 103-113

    • DOI

      10.1111/jbi.12392

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 家庭内二次感染を考慮した下痢症の地域内伝播モデルの構築と検証2014

    • Author(s)
      三浦郁修,渡部徹,渡辺幸三,福士謙介
    • Journal Title

      土木学会論文集G(環境)

      Volume: 70(7)

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Restriction Fragment Length Polymorphism (RFLP) Analysis of Antibiotic Resistant Escherichia coli for Its Source Tracking2014

    • Author(s)
      Toru Watanabe, KoheOzawa, Hiroaki Ito, Ryo Honda, Wilai Chiemchaisri, Kozo Watanabe
    • Journal Title

      Proceedings of 7th ASEAN Environmental Engineering Conference featuring the 5th Seminar on Asian Water Environment

      Volume: -

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Environmental determinants in the control of dengue mosquito vector, Aedes aegypti2014

    • Author(s)
      Thaddeus M. Carvajal and Kozo Watanabe
    • Journal Title

      Proceedings of 7th ASEAN Environmental Engineering Conference featuring the 5th Seminar on Asian Water Environment

      Volume: -

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects on River Macroinvertebrate Communities of Tsunami Propagation after the 2011 Great East Japan Earthquake2014

    • Author(s)
      Kozo Watanabe, Sakiko Yaegashi, Hiro Tomozawa, Shunichi Koshimura, and Tatsuo Omura
    • Journal Title

      Freshwater Biology

      Volume: 59 Issue: 7 Pages: 1474-1483

    • DOI

      10.1111/fwb.12359

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Inferring Fine-scale Dispersal in the Caddisfly Stenopsyche marmorata (Trichoptera; Stenopsychidae) Using Spatial Autocorrelation of Microsatellite Markers2014

    • Author(s)
      Sakiko Yaegashi, Kozo Watanabe, Michael T Monaghan, and Tatsuo Omura
    • Journal Title

      Freshwater Science

      Volume: Vol. 33 Issue: 1 Pages: 172-180

    • DOI

      10.1086/675076

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Climate change detected by molecular ecology and evolution2016

    • Author(s)
      Kozo Watanabe
    • Organizer
      Seminar for Genetics-Ecology-Evolution-Modeling
    • Place of Presentation
      Busan・Korea
    • Year and Date
      2016-03-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Impacts of Climate Change on Dengue in Metro Manila, Philippines2016

    • Author(s)
      Thaddeus M. Carvajal, Howell T. Ho,Yoshiki Kubo, Lara Fides Hernandez, Katherine Viacrusis, Divina Amalin, Kozo Watanabe
    • Organizer
      Symposium on Climate Change Adaptation in Indonesia
    • Place of Presentation
      Balli・Indonesia
    • Year and Date
      2016-03-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The Ecology of Dengue in Bandung, West Java, 2016-20172016

    • Author(s)
      D. Agustian, L. Faridah, A. Berbudi, F. Rinawan, K. Watanabe
    • Organizer
      The 3rdJSPS-AASPP/GRENE-EcoHealth joint International Symposium on Development of International Network on Health Risk Assessment in Urban Area
    • Place of Presentation
      Balli・Indonesia
    • Year and Date
      2016-03-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genetic inference of spatial dispersal pattern of dengue vector mosquito Aedes aegypti in Metro Manila, Philippines2016

    • Author(s)
      Thaddeus M. Carvajal, Kohei Ogishi, Sakiko Yaegeshi, Lara Fides T. Hernandez and Katherine M. Viacrusis
    • Organizer
      The 3rdJSPS-AASPP/GRENE-EcoHealth joint International Symposium on Development of International Network on Health Risk Assessment in Urban Area
    • Place of Presentation
      Balli・Indonesia
    • Year and Date
      2016-03-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Eco-epidemiologic Study of Dengue Vector Mosquito, Aedesaegypti, In The Philippines.2015

    • Author(s)
      Kozo Watanabe
    • Organizer
      Seminar on the Overview of Facts In The Field AgaintsMosquitoes, Dengue Virus, Human And Problems in Bandung City
    • Place of Presentation
      Bandung・Indonesia
    • Year and Date
      2015-12-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Importance of secondary infection within households in epidemic of waterborne infectious diseases2015

    • Author(s)
      Fuminari Miura, Toru Watanabe, Kozo Watanabe, Kensuke Fukushi
    • Organizer
      6th IWA-ASPIRE Conference & Exhibition
    • Place of Presentation
      Beijing, China
    • Year and Date
      2015-09-20 – 2015-09-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Importance of secondary infection within households in epidemic of waterborne infectious diseases2015

    • Author(s)
      Fuminari Miura, Toru Watanabe, Kozo Watanabe, Kensuke Fukushi
    • Organizer
      6th IWA-ASPIRE Conference & Exhibition
    • Place of Presentation
      Beijing・China
    • Year and Date
      2015-09-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マイクロサテライトマーカーを用いたマニラのデング熱媒介蚊の遺伝子流動の評価2015

    • Author(s)
      大岸航平,Thaddeus M. Carvajal,八重樫咲子,渡辺幸三
    • Organizer
      応用生態工学会第19回大会
    • Place of Presentation
      日本大学(郡山市,福島県)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] フィリピンのターラックシティにおけるデング熱媒介蚊のRNAを用いたウイルス検出2015

    • Author(s)
      大岸航平,Thaddeus M. Carvajal,渡辺幸三
    • Organizer
      土木学会四国支部第21回技術研究発表会
    • Place of Presentation
      香川大学(高松市・香川県)
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] フィリピンのターラックシティにおけるデング熱媒介蚊のRNAを用いたウイルス検出2015

    • Author(s)
      大岸航平,Thaddeus M. Carvajal,渡辺幸三
    • Organizer
      土木学会四国支部第21回技術研究発表会
    • Place of Presentation
      徳島大学(徳島県,徳島市)
    • Year and Date
      2015-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Population biology and ecology of dengue vector mosquito, Aedes aegypti in Metro Manila, Philippine and its implications towards vector control and prevention2015

    • Author(s)
      Thaddeus M. Carvajal,渡辺幸三
    • Organizer
      第2回環境水質工学シンポジウム
    • Place of Presentation
      山形大学(山形県・鶴岡市)
    • Year and Date
      2015-05-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] New adaptive perspective in insects2015

    • Author(s)
      Maribet Gamboa, Kozo Watanabe
    • Organizer
      International symposium for frontier of Bioinformatics
    • Place of Presentation
      東京工業大学(東京都・目黒区)
    • Year and Date
      2015-04-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Restriction Fragment Length Polymorphism (RFLP) Analysis of Antibiotic Resistant Escherichia coli for Its Source Tracking2014

    • Author(s)
      Toru Watanabe, Kohei Ozawa, Hiroaki Ito, Ryo Honda, Wilai Chiemchaisri, Kozo Watanabe
    • Organizer
      7th ASEAN Environmental Engineering Conference featuring the 5th Seminar on Asian Water Environment (Asian Core Program of JSPS, NRCT and EEDT)
    • Place of Presentation
      Palawan, Philippines
    • Year and Date
      2014-11-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Environmental Determinants in the Control of Dengue Mosquito Vector, Aedes aegypti2014

    • Author(s)
      Carvajal Thadeus, Watanabe Kozo
    • Organizer
      6th PSP Scientific Meeting
    • Place of Presentation
      Palawan, Philippines
    • Year and Date
      2014-11-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Environmental Determinants in the Control of Dengue Mosquito Vector, Aedes aegypti.2014

    • Author(s)
      Carvajal T.M. and K. Watanabe
    • Organizer
      5th Forum of Network Association of Microbiologists in Ehime
    • Place of Presentation
      愛媛大学(愛媛県・松山市)
    • Year and Date
      2014-10-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] マニラにおけるデング熱媒介蚊の遺伝子流動の評価を目的としたDNA抽出及びPCRの最適化2014

    • Author(s)
      大岸航平,Thaddeus Carvajal,渡辺幸三
    • Organizer
      土木学会四国支部第20回技術研究発表会
    • Place of Presentation
      徳島大学(徳島県,徳島市)
    • Year and Date
      2014-05-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] マニラにおけるデング熱媒介蚊の遺伝子流動評価を目的としたDNA抽出及びPCR の最適化2014

    • Author(s)
      大岸航平,Thaddeus M. Carvajal,渡辺幸三
    • Organizer
      平成26年度土木学会四国支部技術研究発表会
    • Place of Presentation
      徳島市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Developmental Rate of Aedes aegypti in Different Artificial Water-Holding Containers2014

    • Author(s)
      B. L. J. Almanzor and Thaddeus M. Carvajal
    • Organizer
      The 6th PSP Scientific Meeting
    • Place of Presentation
      Manila, Philippines
    • Related Report
      2013 Annual Research Report
  • [Presentation] The Use of Population Genetics on Dengue Mosquitoes for Effective Vector Control in Manila, Philippines2013

    • Author(s)
      Kozo Watanabe, Thaddeus M. Carvajal
    • Organizer
      JSPS-AASPP/GRENE-EcoHealth joint International Symposium on Development of International Network on Health Risk Assessment in Urban Area
    • Place of Presentation
      Hue, Vietnam
    • Related Report
      2013 Annual Research Report
  • [Presentation] Population genetics in the dengue surveillances: Dispersal pattern of mosquitoes2013

    • Author(s)
      Kozo Watanabe
    • Organizer
      JSPS International Symposium on Broadening Perspectives and Methods in Studying Dengue
    • Place of Presentation
      Manila, Philippines
    • Related Report
      2013 Annual Research Report
  • [Presentation] Vector Biology and Studies of Dengue in the Philippines: Implications towards looking into Vector-Pathogen Systems2013

    • Author(s)
      Thaddeus M. Carvajal and Kozo Watanabe
    • Organizer
      JSPS International Symposium on Broadening Perspectives and Methods in Studying Dengue
    • Place of Presentation
      Manila, Philippines
    • Related Report
      2013 Annual Research Report
  • [Presentation] Thaddeus M. Carvajal and Kozo Watanabe2013

    • Author(s)
      Knowing the Vector of Dengue
    • Organizer
      Be Ware , Be Aware: Dengue! By the Societas Vitae of De La Salle University
    • Place of Presentation
      Manila, Philippines
    • Related Report
      2013 Annual Research Report
  • [Presentation] Vector Fitness and Dengue Transmission2013

    • Author(s)
      Thaddeus M. Carvajal and Kozo Watanabe
    • Organizer
      National Institute of Health (NIH) FORUM: Dengue Virus, Mosquito Vector, Climate and the Crucial
    • Place of Presentation
      Manila, Philippines
    • Related Report
      2013 Annual Research Report
  • [Funded Workshop] Seminar for Genetics-Ecology-Evolution-Modeling2016

    • Place of Presentation
      Pusan National University, Busan, Korea
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Funded Workshop] Seminar on the Overview of Facts In The Field AgaintsMosquitoes, Dengue Virus, Human And Problems in Bandung City2015

    • Place of Presentation
      Padjadjaran University, Bandung, Indonesia
    • Year and Date
      2015-12-08
    • Related Report
      2015 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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