| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
From various cold environments, we obtained Pseudomonas mandelii that grows well at low temperatures, bacteria closely related to Paenibacillus odorifer and Bacillus circulans that efficiently reduce iron, and Pseudoalteromonas bacterium that secretes a particular protein in large quantities. For Shewanella sp. HM13, which abundantly produces extracellular membrane vesicles, we established genetic engineering methods. As to Shewanella livingstonensis Ac10 isolated from Antarctic seawater, we found cold-inducible promoters that may be useful for expression of foreign proteins in this strain. We confirmed that PhoE, an outer membrane protein, is involved in iron metabolism of this bacterium and identified functionally important amino acid residues of this protein.
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