Establishment of worldwide taxonomic system of the genus Volvox and unveiling the evolution of oogamy

• Project/Area Number: 25304012
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Biodiversity/Systematics
• Research Institution: The University of Tokyo
• Principal Investigator: Nozaki Hisayoshi 東京大学, 大学院理学系研究科(理学部), 准教授 (40250104)
• Co-Investigator(Kenkyū-buntansha): 関本 弘之 日本女子大学, 理学部, 教授 (20281652) ; 三角 修己 山口大学, 大学院創成科学研究科, 准教授 (90583625)
• Co-Investigator(Renkei-kenkyūsha): TSUCHIKANE Yuki 日本女子大学, 理学部, 助教 (20434152)
• Project Period (FY): 2013-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: ボルボックス / 分類体系 / 卵生殖 / 進化 / 多様性 / Volvox / 種分類 / 有性生殖 / 性特異的遺伝子 / Volvox carteri / Pleodorina / ボルボックス科

Outline of Final Research Achievements

The present study was undertaken to establish a worldwide, objective and natural taxonomic system of the oogamous genus Volvox and unveiling the evolution of oogamy, based on the molecular and asexual and sexual spheroid morphology of Volvox species collected in various localities of the world. Our field and laboratory studies in this research project resulted in the reconstruction of four, monophyletic infrageneric taxa of the genus Volvox. We re-discovered of the ancestral Volvox species from Thailand and resolved its phylogenyetic position. We identified a novel colonial volvocine genus, Colemanosphaera, in obligately anisogamous conjugation between male and female motile gametes occurred outside the female colony (external fertilization during anisogamy). Considering the external obligate anisogamy, oogamy evolution may have been preceded by the transition from external to internal fertilization during anisogamy within the volvocine green algae.

Research Products

• [Int'l Joint Research] Academia Sinica(台湾)
• [Int'l Joint Research] Khon Kaen University(タイ)
• [Int'l Joint Research] Georgia Institute of Technology(米国)
• [Int'l Joint Research] コンケン大学(タイ)
• [Int'l Joint Research] カンザス州立大学(米国)
• [Int'l Joint Research] 生物多様性中心(台湾)
• [Int'l Joint Research] コンケーン大学(タイ)
• [Int'l Joint Research] モンタナ大学(米国)
• [Journal Article] Ultrastructure of the marine benthic dinoflagellate Plagiodinium belizeanum (Dinophyceae) from the southeast Pacific island of Okinawa, Japan (2018)
  • Author(s): Wakeman Kevin C.、Hoppenrath Mona、Yamaguchi Aika、Gavelis Greg S.、Leander Brian S.、Nozaki Hisayoshi
  • Journal Title: Phycologia Volume: 57 Issue: 2 Pages: 209-222
  • DOI: 10.2216/17-43.1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Morphology, taxonomy and mating-type loci in natural populations of Volvox carteri in Taiwan (2018)
  • Author(s): Nozaki, H., Ueki, N., Takusagawa, M., Yamashita, S. Misumi, O., Matsuzaki, R., Kawachi, M., Chiang, Y.-R. and Wu, J.-T.
  • Journal Title: Botanical Studies Volume: 59 Issue: 1 Pages: 10-10
  • DOI: 10.1186/s40529-018-0227-9
  • NAID: 120006532083
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Taxonomic revision of Chloromonas nivalis (Volvocales, Chlorophyceae) strains, with the new description of two snow-inhabiting Chloromonas species (2018)
  • Author(s): Matsuzaki R, Nozaki H, Kawachi M.
  • Journal Title: PLoS ONE Volume: 3 Issue: 3 Pages: e0193603-e0193603
  • DOI: 10.1371/journal.pone.0193603
  • Peer Reviewed / Open Access
• [Journal Article] Rediscovery of the “ancestral Volvox” species: Morphology and phylogenetic position of Pleodorina sphaerica (Volvocales, Chlorophyceae) from Thailand. (2017)
  • Author(s): Nozaki, H., Mahakham, W., Athibai, S., Yamamoto, K., Takusagawa, M., Misumi, O., Herron, M. D., Rosenzweig, F., Kawachi, M.
  • Journal Title: Phycologia Volume: 56 Issue: 4 Pages: 469-475
  • DOI: 10.2216/17-3.1
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Sequence of the Gonium pectorale Mating Locus Reveals a Complex and Dynamic History of Changes in Volvocine Algal Mating Haplotypes. (2016)
  • Author(s): Hamaji T, Mogi Y, Ferris PJ, Mori T, Miyagishima S, Kabeya Y, Nishimura Y, Toyoda A, Noguchi H, Fujiyama A, Olson BJ, Marriage TN, Nishii I, Umen JG, Nozaki H.
  • Journal Title: G3 Volume: 未定 Issue: 5 Pages: 1179-1189
  • DOI: 10.1534/g3.115.026229
  • NAID: 120005997691
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular phylogeny of the marine planktonic dinoflagellate Oxytoxum and Corythodinium (Peridiniales, Dinophyceae). (2016)
  • Author(s): Gomez, F., Wakeman, K. C., Yamaguchi, A., Nozaki, H.
  • Journal Title: Molecular phylogeny of the marine planktonic dinoflagellate Oxytoxum and Corythodinium (Peridiniales, Dinophyceae). Volume: 55 Issue: 4 Pages: 239-248
  • DOI: 10.4467/16890027ap.16.026.6095
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A New Morphological Type of Volvox from Japanese Large Lakes and Recent Divergence of this Type and V. ferrisii in Two Different Freshwater Habitats. (2016)
  • Author(s): Nozaki H, Ueki N, Isaka N, Saigo T, Yamamoto K, Matsuzaki R, Takahashi F, Wakabayashi K, Kawachi M.
  • Journal Title: PLoS One Volume: 11 Issue: 11 Pages: e0167148-e0167148
  • DOI: 10.1371/journal.pone.0167148
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Taxonomic revision of Chlamydomonas subg. Amphichloris (Volvocales, Chlorophyceae) with resurrection of genus Dangeardinia Temp. and descriptions of Ixipapillifera gen. nov. and Rhysamphichloris gen. nov. (2016)
  • Author(s): Nakada, T., Tomita, M., Wu, J.-T., Nozaki, H.
  • Journal Title: J. Phycol. Volume: 52 Issue: 2 Pages: 283-304
  • DOI: 10.1111/jpy.12397
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Morphology and reproduction of Volvox capensis (Volvocales, Chlorophyceae) from Montana, USA. (2015)
  • Author(s): Nozaki, H., Ueki, N., Misumi, O., Yamamoto, K., Yamashita, S., Herron, M. D. and Rosenzweig, F.
  • Journal Title: Phycologia Volume: 54 Issue: 3 Pages: 316-320
  • DOI: 10.2216/15-14.1
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Morphology and phylogenetic position of a rare four-celled green alga, Pascherina tetras (Volvocales, Chlorophyceae), based on cultured material. (2015)
  • Author(s): Sugasawa, M., Matsuzaki, R., Arakaki, Y. and Nozaki, H.
  • Journal Title: Phycologia Volume: 54 Issue: 4 Pages: 342-348
  • DOI: 10.2216/15-27.1
  • Peer Reviewed
• [Journal Article] Taxonomic Study of <i>Pyrobotrys</i> (Spondylomoraceae, Chlorophyceae) Based on Comparative Morphological and Molecular Analyses of Culture Strains Established Using Novel Methods (2015)
  • Author(s): Sugasawa, M., Matsuzaki, R., Kawafune, K., Takahashi, T., Kawachi, M., Krienitz, L. and Nozaki, H.
  • Journal Title: CYTOLOGIA Volume: 80 Issue: 4 Pages: 513-524
  • DOI: 10.1508/cytologia.80.513
  • NAID: 130005118539
  • ISSN: 0011-4545, 1348-7019
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Delineating a new heterothallic species of Volvox (volvocaceae, chlorophyceae) using new strains of “Volvox africanus” (2015)
  • Author(s): Nozaki H, Matsuzaki R, Yamamoto K, Kawachi M, Takahashi F
  • Journal Title: PLOS One Volume: 10 Issue: 11 Pages: 0142632-0142632
  • DOI: 10.1371/journal.pone.0142632
  • Peer Reviewed / Open Access
• [Journal Article] A rickettsial endosymbiont inhabiting the cytoplasm of Volvox carteri (Volvocales, Chlorophyceae). (2014)
  • Author(s): Kawafune, K., Hongoh, Y. and Nozaki, H.
  • Journal Title: Phycologia Volume: 53 Issue: 1 Pages: 95-99
  • DOI: 10.2216/13-193.1
  • Peer Reviewed
• [Journal Article] Sex-specific post-translational regulation of the gamete fusogen GCSI in the isogamous volvocine alga Gonium pectorale. (2014)
  • Author(s): Kawai-Toyooka H, Mori T, Hamaji T, Suzuki M, Olson BJ, Uemura T, Ueda T, Nakano A, Toyoda A, Fujiyama A, Nozaki H
  • Journal Title: EUkaryotic Cell Volume: (in press) Issue: 5 Pages: 648-656
  • DOI: 10.1128/ec.00330-13
  • Peer Reviewed
• [Journal Article] taxonomic study of snow Chloromonas species (Volvocales, Chlorophyceae) based on light and electron microscopy and molecular analysis of cultured material. (2014)
  • Author(s): Matsuzaki, R., Hara, Y. and Nozaki, H.
  • Journal Title: Phycologia Volume: 53 Issue: 3 Pages: 293-304
  • DOI: 10.2216/14-3.1
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Surface Ornamentation of <i>Cyanophora paradoxa</i> (Cyanophorales, Glaucophyta) Cells as Revealed by Ultra-High Resolution Field Emission Scanning Electron Microscopy (2014)
  • Author(s): Takahashi, T., Sato, M., Toyooka, K. and Nozaki, H.
  • Journal Title: CYTOLOGIA Volume: 79 Issue: 1 Pages: 119-123
  • DOI: 10.1508/cytologia.79.119
  • NAID: 130004448264
  • ISSN: 0011-4545, 1348-7019
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] New "missing link" genus of the colonial volvocine green algae gives insights into the evolution of oogamy. (2014)
  • Author(s): Nozaki H., Yamada T. K., Takahashi F., Matsuzaki R., & Nakada T.
  • Journal Title: BMC Evolutionary Biology Volume: 14 Issue: 1 Pages: 37-37
  • DOI: 10.1186/1471-2148-14-37
  • Peer Reviewed
• [Journal Article] Morphological and molecular evidence support the recognition of Champia lubrica sp. nov. (Champiaceae, Rhodophyta) from Japan. (2013)
  • Author(s): Suzuki, M., Hashimoto, T., Kitayama, T. and Nozaki, H.
  • Journal Title: Phycologia Volume: 52 Issue: 6 Pages: 609-617
  • DOI: 10.2216/13-128.1
  • Peer Reviewed
• [Presentation] Volvocine green algae inviting you to 200 MYA time travel for evolution of female-male and muticellularity (2018)
  • Author(s): Nozaki, H.
  • Organizer: Biology Colloquium, Department of Biological Sciences, Faulty of Science, National University of Singapore
  • Invited
• [Presentation] タイ王国産最大級ボルボックスと最小ボルボックス？ (2017)
  • Author(s): 野崎久義、Wuttipong Mahakham、Sujeephon Athibai、田草川真理、三角修己、河地正伸
  • Organizer: 日本藻類学会第41回大会
  • Place of Presentation: 高知大学朝倉キャンパス（高知県高知市）
  • Year and Date: 2017-03-24
• [Presentation] 彩雪を構成する氷雪性緑藻類のシストの分子系統と１未記載 (2017)
  • Author(s): 松﨑令、野崎久義、竹内望、原慶明、河地正伸
  • Organizer: 日本植物分類学会第16 回大会
  • Place of Presentation: 京都大学吉田キャンパス（京都府京都市）
  • Year and Date: 2017-03-10
• [Presentation] タイ王国産ボルボックス類２種 (2017)
  • Author(s): 野崎久義、Wuttipong Mahakham、Sujeephon Athibai、田草川真理、三角修己、河地正伸
  • Organizer: 日本植物分類学会第16 回大会
  • Place of Presentation: 京都大学吉田キャンパス（京都府京都市）
  • Year and Date: 2017-03-10
• [Presentation] 培養株と野外サンプルに基づく氷雪性緑藻の１未記載種の分類学的研究 (2017)
  • Author(s): 松﨑令、野崎久義、竹内望、原慶明、河地正伸
  • Organizer: 日本植物学会第81回大会
• [Presentation] 台湾産の Volvox carteri (2017)
  • Author(s): 野崎久義、植木紀子、田草川真里、三角修己、Chiang Yin-Ru、Wu Jiunn-Tzong
  • Organizer: 日本植物学会第81回大会
• [Presentation] Volvox carteri from Taiwan (2017)
  • Author(s): Nozaki, H. Ueki, N., Takusagawa, M., Yamashita, S., Misumi, O., Yin-Ru Chiang, Y.-R. and Wu, J.-T.
  • Organizer: The Fourth International Volvox Conference
  • Int'l Joint Research
• [Presentation] Taxonomy of a new snow-inhabiting species of Chloromonas based on the use of cultured and field-collected materials (2017)
  • Author(s): Matsuzaki, R., Nozaki, H., Takeuchi, N., Hara, Y. and Kawachi, M.
  • Organizer: The Fourth International Volvox Conference
  • Int'l Joint Research
• [Presentation] Establishing new strains of Volvox for future studies of biodiversity and evolution (2016)
  • Author(s): Nozaki, H., Mahakham, W., Athibai, S., Takusagawa, M., Misumi, M.
  • Organizer: The 69th Yamada Conference 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: 京都国際会議場（京都府京都市）
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] 日本の湖沼に生育するボルボックス節の1種について (2016)
  • Author(s): 野崎久義、井坂奈々子、西郷永希子、山本荷葉子、松﨑令、高橋文雄
  • Organizer: 日本植物分類学会第15回大会
  • Place of Presentation: 富山大学五福キャンパス、富山市
  • Year and Date: 2016-03-06
• [Presentation] ボルボックスの仲間を用いた多細胞化と雌雄性の進化 (2016)
  • Author(s): 野崎久義
  • Organizer: 第４回細胞凝集研究会
  • Place of Presentation: 札幌全日空ホテル（北海道札幌市）
  • Invited
• [Presentation] Taxonomic re-examination of two sexual types of “Volvox africanus” by Starr (1971), based on the use of new strains from Lake Biwa, Japan (2015)
  • Author(s): Nozaki, H., Matsuzaki, R., Yamamoto, K. and Takahashi, F
  • Organizer: Third International Volvox Conference
  • Place of Presentation: Centre for Mathematical Sciences, University of Cambridge, Cambridge, United Kingdom
  • Year and Date: 2015-09-19
  • Int'l Joint Research
• [Presentation] 日本新産と思われる琵琶湖産ボルボックス2種の形態と有性生殖の観察と種分類 (2015)
  • Author(s): 野崎久義、松﨑令、山本荷葉子、高橋文雄
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ：新潟コンベンションセンター、新潟市
  • Year and Date: 2015-09-06
• [Presentation] 新規培養株を用いた”Volvox africanus” のsexual types (Starr 1971) の再調査 (2015)
  • Author(s): 野崎久義、松﨑令、山本荷葉子、高橋文雄
  • Organizer: 第12回クラミドモナス研究会
  • Place of Presentation: 中央大学理工学部、東京都
  • Year and Date: 2015-09-03
• [Presentation] 初めて南アフリカ以外から採取されたVolvox capensis Rich & Pocock (1933) (2015)
  • Author(s): 野崎久義、植木紀子、三角修己、Matthew Herron
  • Organizer: 日本植物分類学会第14回大会
  • Place of Presentation: 福島大学、福島市
  • Year and Date: 2015-03-06 – 2015-03-08
• [Presentation] ボルボックスの仲間を用いて探るメスとオスの進化 (2014)
  • Author(s): 野崎久義
  • Organizer: 自然史学会連合講演会「第一線の研究者が語る進化の謎」
  • Place of Presentation: ミュージアムパーク茨城県自然博物館、茨城県坂東市
  • Year and Date: 2014-11-23
  • Invited
• [Presentation] 群体性藻類Stephanosphaera（緑藻綱，オオヒゲマワリ目）の単細胞性姉妹群の再検討 (2014)
  • Author(s): 宗像英仁、仲田崇志、野崎久義、冨田勝
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学生田キャンパス、川崎市
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] 緑藻群体性ボルボックス目の新属２種の分類と分布 (2014)
  • Author(s): 野崎久義、山田敏寛、高橋文雄、松崎令、仲田崇志
  • Organizer: 日本微生物資源学会第21回大会
  • Place of Presentation: 京農業大学世田谷キャンパス、東京都
  • Year and Date: 2014-09-03 – 2014-09-04
• [Presentation] 群体性ボルボックス目の “ミッシングリンク” 新属” (2014)
  • Author(s): 野崎久義、山田敏寛、高橋文雄、松崎令、仲田崇志
  • Organizer: 日本植物分類学会第13回大会
  • Place of Presentation: 熊本大学黒髪南キャンパス、熊本市
• [Presentation] 配偶子進化モデル系統群「群体性ボルボックス目」の異型配偶子体外接合 (2014)
  • Author(s): 野崎久義
  • Organizer: 日本藻類学会第38回大会
  • Place of Presentation: 東邦大学習志野キャンパス、船橋市
• [Presentation] Chlamydomonas subg. Amphichloris（緑藻綱，オオヒゲマワリ目）の分類学的見直しとミトコンドリア形態の多様性 (2014)
  • Author(s): 仲田崇志、野崎久義、冨田勝
  • Organizer: 日本藻類学会第38回大会
  • Place of Presentation: 東邦大学習志野キャンパス、船橋市
• [Remarks] 三陸山田町で発見した新種ナンブワツナギソウ
  • URL: http://www.s.u-tokyo.ac.jp/ja/press/2013/49.html