Elucidation of male sterility occurred in cattle-yak hybrid and production of calf from the hybrid male in Mongolia

• Project/Area Number: 25304039
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Animal production science
• Research Institution: The University of Tokushima (2016); Yamaguchi University (2013-2015)
• Principal Investigator: Otoi Takeshige 徳島大学, 大学院生物資源産業学研究部, 教授 (30311814)
• Co-Investigator(Kenkyū-buntansha): 佐藤 陽子 東亜大学, 医療学部, 教授 (50398963)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000); Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: 繁殖 / 精子形成 / 不妊 / 交雑種 / ヤク / モンゴル

Outline of Final Research Achievements

In Mongolia, Yak (Bos grunniens) live in the alpine area, and their products are indispensable resources for local people. Crossbreeding with cattle (Bos taurus) gives dominant trait. However, male hybrid shows infertility because of spermatogenetic arrest (male sterility). This study investigated spermatogenesis of cattle-yak hybrid and evaluated the effects of detergent Orvus ES Paste (OEP) on the post-thaw motility and viability of epididymal and ejaculated yak spermatozoa. Our findings indicate that the male sterility results from low ability of cell death but not from cell growth in the spermatid. Moreover, the addition of 0.75% OEP was effective for the preservation of yak spermatozoa.

Research Products

• [Int'l Joint Research] 広東海洋大学(中国)
• [Journal Article] Effects of parity and season on pregnancy rates after the transfer of embryos to repeat-breeder Japanese Black beef cattle. (2016)
  • Author(s): Ono, T., Isobe, T., Morita, Y., Do, T.K.L., Tanihara, F., Taniguchi, M., Takagi, M. and Otoi, T.
  • Journal Title: Arch. Anim. Breed. Volume: 59 Issue: 1 Pages: 45-49
  • DOI: 10.5194/aab-59-45-2016
  • Peer Reviewed
• [Journal Article] Effects of Orvus ES Paste on the motility and viability of yak (Bos grunniens) epididymal and ejaculated spermatozoa after freezing and thawing. (2015)
  • Author(s): Shimazaki, M., Sambuu, R., Sato, Y., Do, T.K.L., Tanihara, F., Taniguchi, M. and Otoi, T.
  • Journal Title: CryoLetters Volume: 36 Pages: 264-269
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Formation of an Antral Follicle-Like Structure by Bovine Cumulus-Oocyte Complexes Embedded with Fragmin/Protamine Microparticles. (2015)
  • Author(s): Morita Y, Karja NW, Do LT, Tanihara F, Taniguchi M, Otoi T
  • Journal Title: Anim Biotechnol. Volume: 26 Issue: 4 Pages: 273-275
  • DOI: 10.1080/10495398.2015.1015681
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effects of dibutyryl-cyclic adenosine monophosphate and human chorionic gonadotropin on the formation of antral follicle-like structure by bovine cumulus-oocyte complexes. (2015)
  • Author(s): Ono, T., Takaoka, A., Morita, Y., Do, T.K.L., Tanihara, F., Taniguchi, M. and Otoi, T.
  • Journal Title: Acta Vet. Hung. Volume: 63 Issue: 4 Pages: 485-498
  • DOI: 10.1556/004.2015.045
  • Peer Reviewed
• [Journal Article] In vitro development of OPU-derived bovine embryos cultured either individually or in groups with the silk protein sericin and the viability of frozen-thawed embryos after transfer. (2015)
  • Author(s): Isobe T, Ikebata Y, Do LT, Tanihara F, Taniguchi M, Otoi T.
  • Journal Title: Anim Sci J. Volume: 86 Issue: 7 Pages: 661-665
  • DOI: 10.1111/asj.12341
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Superovulatory response and pregnancies after interspecies transfer of embryos in semi-wild Dulong (Bos frontalis). (2014)
  • Author(s): He, Z.X., Sato, Y., Zhang, J.C., Huang, B.Z., Wittayarat, M., Taniguchi, M., Suzuki, T. and Otoi, T.
  • Journal Title: Veterinarski arhiv Volume: 84 Pages: 183-188
  • Peer Reviewed
• [Journal Article] Nuclear status and DNA fragmentation of oocytes from porcine, bovine and feline ovaries stored at 4°C for 5 days. (2014)
  • Author(s): Luu, V.V., Namula, Z., Do, T.K.L., Sato, Y., Taniguchi, M., Karja, N.W.K. and Otoi, T.
  • Journal Title: CryoLetters Volume: 35 Pages: 48-53
  • Peer Reviewed
• [Journal Article] Cryopreservation for bovine embryos in serum-free freezing medium containing silk protein sericin. (2013)
  • Author(s): Isobe T, Ikebata Y, Onitsuka T, Do LT, Sato Y, Taniguchi M, Otoi T.
  • Journal Title: Cryobiology. Volume: 67 (2) Issue: 2 Pages: 184-187
  • DOI: 10.1016/j.cryobiol.2013.06.010
  • Peer Reviewed