Epidemiological survey of protozoan diseases in Mongolia and their impact against cold weather.

• Project/Area Number: 25304041
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Veterinary medical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: IGARASHI Makoto 帯広畜産大学, 原虫病研究センター, 教授 (60374766)
• Co-Investigator(Kenkyū-buntansha): INOUE Noboru 帯広畜産大学, 原虫病研究センター, 教授 (10271751) ; YOKOYAMA Naoaki 帯広畜産大学, 原虫病研究センター, 教授 (80301802)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: モンゴル / トキソプラズマ / ネオスポラ / バベシア / タイレリア / トリパノソーマ / クリプトスポリジウム

Outline of Final Research Achievements

Epidemiological survey of chronic protozoan diseases including Toxoplasma, Neospora, Cryptosporidium, Babesia, Theileria, and Trypanosoma were conducted in Mongolia. Seroprevalences of Toxoplasma and Neospora were about 10% in cattle, 40-50% in sheep and goat, and negative in horse and camel. Infection rates of Babesia caballi and Theileria equi in horse were about 50% and 20%, respectively. Tick with Babesia microti infection was about 30%. Genotyping of Babesia bovis was performed for developing molecular diagnostic tool. Immunochromatography was prepared for diagnosis of Trypanosoma infection. Isolation and in vitro culture of Trypanosoma equiperdum were successfully established.

Research Products

• [Int'l Joint Research] モンゴル農業大学獣医学研究所(モンゴル)
• [Journal Article] Serological Survey and Factors Associated with Toxoplasma gondii Infection in Domestic Goats in Myanmar (2016)
  • Author(s): Bawm Saw, Maung Wint Yi, Win Myat Yee, Thu May June, Chel Hla Myet, Khaing Tin Aye, Wai Soe Soe, Htun Lat Lat, Myaing Tin Tin, Tiwananthagorn Saruda, Igarashi Makoto, and Katakura Ken
  • Journal Title: Scientifica Volume: 2016 Pages: 1-4
  • DOI: 10.1155/2016/4794318
  • NAID: 120005725149
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Type-specific PCR assays for Babesia bovis msa-1 genotypes in Asia: Revisiting the genetic diversity in Sri Lanka, Mongolia, and Vietnam. (2016)
  • Author(s): Liyanagunawardena N, Sivakumar T, Kothalawala H, Silva SS, Battsetseg B, Lan DT, Inoue N, Igarashi I, Yokoyama N.
  • Journal Title: Infect Genet Evol. Volume: 37 Pages: 64-69
  • DOI: 10.1016/j.meegid.2015.10.029
  • NAID: 120006390924
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The PCR detection and phylogenetic characterization of Babesia microti in questing ticks in Mongolia. (2015)
  • Author(s): Tuvshintulga B, Sivakumar T, Battsetseg B, Narantsatsaral SO, Enkhtaivan B, Battur B, Hayashida K, Okubo K, Ishizaki T, Inoue N, Igarashi I, Yokoyama N.
  • Journal Title: Parasitol Int. Volume: 64 Issue: 6 Pages: 527-532
  • DOI: 10.1016/j.parint.2015.07.007
  • NAID: 120006390873
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A TeGM6-4r antigen-based immunochromatographic test (ICT) for animal trypanosomosis (2015)
  • Author(s): Nguyen, T. T., Ruttayaporn, N., Goto, Y., Kawazu, S., Sakurai, T., Inoue, N.
  • Journal Title: Parasitology Research Volume: 114 Issue: 11 Pages: 4319-4325
  • DOI: 10.1007/s00436-015-4672-z
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structural and functional divergence of the aldolase fold in Toxoplasma gondii. (2015)
  • Author(s): Tonkin, M.L., Halavaty, A.S., Ramaswamy, R., Ruan, J., Igarashi, M., Ngô, H.M., Boulanger, M.J.
  • Journal Title: J. Mol. Biol. Volume: 427 Issue: 4 Pages: 840-852
  • DOI: 10.1016/j.jmb.2014.09.019
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Seroprevalences of <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> in Pet Rabbits in Japan (2014)
  • Author(s): Salman, D., Oohashi, E., Mohamed, A.E.E., El-Mottelib, A.E.A., Okada, T., Igarashi, M.
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Issue: 6 Pages: 855-862
  • DOI: 10.1292/jvms.13-0632
  • NAID: 130003391296
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Identification and Characterization of a <i>Trypanosoma congolense</i> 46 kDa Protein as a Candidate Serodiagnostic Antigen (2014)
  • Author(s): Zhou, M., Suganuma, K., Ruttayaporn, N., Nguyen, T.T., Yamasaki, S., Igarashi, I., Kawazu, S., Suzuki, Y., Inoue, N.
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Issue: 6 Pages: 799-806
  • DOI: 10.1292/jvms.13-0462
  • NAID: 130003391280
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Diagnostic value of the recombinant tandem repeat antigen TeGM6-4r for surra in water buffaloes (2014)
  • Author(s): Nguyen TT, Zhou M, Ruttayaporn N, Nguyen QD, Nguyen VK, Goto Y, Suzuki Y, Kawazu SI, Inoue N
  • Journal Title: Vet Parasitol Volume: 201 Issue: 1-2 Pages: 18-23
  • DOI: 10.1016/j.vetpar.2014.01.009
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of a fluorescence-based method for antibabesial drug screening. (2014)
  • Author(s): Guswanto A, Sivakumar T, Rizk MA, Elsayed SA, Youssef MA, ElSaid Eel S, Yokoyama N, Igarashi I.
  • Journal Title: Antimicrob. Agents Chemother. Volume: 58 Issue: 8 Pages: 4713-4717
  • DOI: 10.1128/aac.00022-14
  • Peer Reviewed / Open Access
• [Journal Article] PCR detection of Babesia ovata from questing ticks in Japan. (2014)
  • Author(s): Sivakumar T, Tattiyapong M, Okubo K, Suganuma K, Hayashida K, Igarashi I, Zakimi S, Matsumoto K, Inokuma H, Yokoyama N.
  • Journal Title: Ticks Tick Borne Dis. Volume: 5 Issue: 3 Pages: 305-310
  • DOI: 10.1016/j.ttbdis.2013.12.006
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Expression, immunolocalization and serodiagnostic value of Tc38630 protein from Trypanosoma congolense (2013)
  • Author(s): Mochabo, K. M., Zhou, M., Suganuma, K., Kawazu, S., Suzuki, Y. and Inoue, N.
  • Journal Title: Parasitology Research Volume: 112 Issue: 9 Pages: 3357-3363
  • DOI: 10.1007/s00436-013-3515-z
  • Peer Reviewed
• [Journal Article] Prevalence and genetic diversity of equine piroplasms in Tov province, Mongolia. (2013)
  • Author(s): Munkhjargal T, Sivakumar T, Battsetseg B, Nyamjargal T, Aboulaila M, et al.
  • Journal Title: Infection, Genetics and Evolution Volume: 16 Pages: 178-185
  • DOI: 10.1016/j.meegid.2013.02.005
  • Peer Reviewed
• [Journal Article] A PCR based survey of Babesia ovata in cattle from various Asian, African and South American countries. (2013)
  • Author(s): Yoshinari T, Sivakumar T, Asada M, Battsetseg B, Huang X, Lan DT, Inpankaew T, Ybañez AP, Alhassan A, Thekisoe OM, De Macedo AC, Inokuma H, Igarashi I, Yokoyama N.
  • Journal Title: J Vet Med Sci. Volume: 75 Pages: 211-214
  • NAID: 130001879670
  • Peer Reviewed
• [Journal Article] A novel dense granule protein, GRA22, is involved in regulating parasite egress in Toxoplasma gondii. (2013)
  • Author(s): Okada T, Marmansari D, Li ZM, Adilbish A, Canko S, Ueno A, Shono H, Furuoka H, Igarashi M.
  • Journal Title: Mol Biochem Parasitol. Volume: 189 Issue: 1-2 Pages: 5-13
  • DOI: 10.1016/j.molbiopara.2013.04.005
  • Peer Reviewed
• [Presentation] Analysis of theRoleof Toxoplasma gondiiLactate dehydrogenase during stage conversion using Knockout techniques. (2016)
  • Author(s): Abdelbaset Abdelbaset、五十嵐 慎
  • Organizer: 第８５回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県・宮崎市）
  • Year and Date: 2016-03-18
• [Presentation] トキソプラズマのシスト形成に寄与する因子の探索 (2016)
  • Author(s): 村田 優穂、高野 量、五十嵐 慎、加藤 健太郎
  • Organizer: 第８５回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県・宮崎市）
  • Year and Date: 2016-03-18
• [Presentation] PCRdetectionandphylogeneticcharacterizationofBabesia microtiinquestingticksin Mongolia (2015)
  • Author(s): BumduurenTuvshintulga、SivakumarThillaiampalam、横山 直明、五十嵐 郁男
  • Organizer: 第１５８回日本獣医学会学術集会
  • Place of Presentation: 北里大学獣医学部（青森県・十和田市）
  • Year and Date: 2015-09-07
• [Presentation] A TeGM6-4r antigen based immunochromatographic test (ICT) for diagnosis of animal trypanosomosis (2014)
  • Author(s): Thu-Thuy Nguyen, Ngasaman Ruttayaporn, Yasuyuki Goto, Zhao-Rong Lun, Shin-ichiro Kawazu, Charles P. Othim, Heriel A. Mbwambo, Chihiro Sugimoto and Noboru Inoue
  • Organizer: 18th Japanese-German Cooperative Symposium on Protozoan Diseases
  • Place of Presentation: Technische Universitat, dresden
  • Year and Date: 2014-09-30 – 2014-10-03
• [Presentation] Prevalence and genetic characterization of Toxoplasma gondii isolate from Tokachi subprefecture in Japan. (2014)
  • Author(s): Salman Doaa、大橋英二、Abdelbaset Abdelbaset E、五十嵐慎
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] Genetic diversity of Theileria (2014)
  • Author(s): 横山直明、Sivakumar Thillaiamapalam、林田京子、杉本千尋
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] Babesia ovata:Epidemiology and clinical significance (2014)
  • Author(s): 横山直明、Sivakumar Thillaiampalam、五十嵐郁男、猪熊壽
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛大学
• [Presentation] Identification and Characterization of novel bradyzoite-specific genes in Toxoplasma gondii (2013)
  • Author(s): Doaa Salman、Muller Compaore、Suphasawatt Puranaveja、岡田只士、五十嵐慎
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学