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Epidemiological survey of protozoan diseases in Mongolia and their impact against cold weather.

Research Project

Project/Area Number 25304041
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section海外学術
Research Field Veterinary medical science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

IGARASHI Makoto  帯広畜産大学, 原虫病研究センター, 教授 (60374766)

Co-Investigator(Kenkyū-buntansha) INOUE Noboru  帯広畜産大学, 原虫病研究センター, 教授 (10271751)
YOKOYAMA Naoaki  帯広畜産大学, 原虫病研究センター, 教授 (80301802)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywordsモンゴル / トキソプラズマ / ネオスポラ / バベシア / タイレリア / トリパノソーマ / クリプトスポリジウム
Outline of Final Research Achievements

Epidemiological survey of chronic protozoan diseases including Toxoplasma, Neospora, Cryptosporidium, Babesia, Theileria, and Trypanosoma were conducted in Mongolia. Seroprevalences of Toxoplasma and Neospora were about 10% in cattle, 40-50% in sheep and goat, and negative in horse and camel. Infection rates of Babesia caballi and Theileria equi in horse were about 50% and 20%, respectively. Tick with Babesia microti infection was about 30%. Genotyping of Babesia bovis was performed for developing molecular diagnostic tool. Immunochromatography was prepared for diagnosis of Trypanosoma infection. Isolation and in vitro culture of Trypanosoma equiperdum were successfully established.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (23 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (1 results) Journal Article (14 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 14 results,  Open Access: 10 results,  Acknowledgement Compliant: 4 results) Presentation (8 results)

  • [Int'l Joint Research] モンゴル農業大学獣医学研究所(モンゴル)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Serological Survey and Factors Associated with Toxoplasma gondii Infection in Domestic Goats in Myanmar2016

    • Author(s)
      Bawm Saw, Maung Wint Yi, Win Myat Yee, Thu May June, Chel Hla Myet, Khaing Tin Aye, Wai Soe Soe, Htun Lat Lat, Myaing Tin Tin, Tiwananthagorn Saruda, Igarashi Makoto, and Katakura Ken
    • Journal Title

      Scientifica

      Volume: 2016 Pages: 1-4

    • DOI

      10.1155/2016/4794318

    • NAID

      120005725149

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Type-specific PCR assays for Babesia bovis msa-1 genotypes in Asia: Revisiting the genetic diversity in Sri Lanka, Mongolia, and Vietnam.2016

    • Author(s)
      Liyanagunawardena N, Sivakumar T, Kothalawala H, Silva SS, Battsetseg B, Lan DT, Inoue N, Igarashi I, Yokoyama N.
    • Journal Title

      Infect Genet Evol.

      Volume: 37 Pages: 64-69

    • DOI

      10.1016/j.meegid.2015.10.029

    • NAID

      120006390924

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The PCR detection and phylogenetic characterization of Babesia microti in questing ticks in Mongolia.2015

    • Author(s)
      Tuvshintulga B, Sivakumar T, Battsetseg B, Narantsatsaral SO, Enkhtaivan B, Battur B, Hayashida K, Okubo K, Ishizaki T, Inoue N, Igarashi I, Yokoyama N.
    • Journal Title

      Parasitol Int.

      Volume: 64 Issue: 6 Pages: 527-532

    • DOI

      10.1016/j.parint.2015.07.007

    • NAID

      120006390873

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A TeGM6-4r antigen-based immunochromatographic test (ICT) for animal trypanosomosis2015

    • Author(s)
      Nguyen, T. T., Ruttayaporn, N., Goto, Y., Kawazu, S., Sakurai, T., Inoue, N.
    • Journal Title

      Parasitology Research

      Volume: 114 Issue: 11 Pages: 4319-4325

    • DOI

      10.1007/s00436-015-4672-z

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Structural and functional divergence of the aldolase fold in Toxoplasma gondii.2015

    • Author(s)
      Tonkin, M.L., Halavaty, A.S., Ramaswamy, R., Ruan, J., Igarashi, M., Ngô, H.M., Boulanger, M.J.
    • Journal Title

      J. Mol. Biol.

      Volume: 427 Issue: 4 Pages: 840-852

    • DOI

      10.1016/j.jmb.2014.09.019

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Seroprevalences of <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> in Pet Rabbits in Japan2014

    • Author(s)
      Salman, D., Oohashi, E., Mohamed, A.E.E., El-Mottelib, A.E.A., Okada, T., Igarashi, M.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 76 Issue: 6 Pages: 855-862

    • DOI

      10.1292/jvms.13-0632

    • NAID

      130003391296

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification and Characterization of a <i>Trypanosoma congolense</i> 46 kDa Protein as a Candidate Serodiagnostic Antigen2014

    • Author(s)
      Zhou, M., Suganuma, K., Ruttayaporn, N., Nguyen, T.T., Yamasaki, S., Igarashi, I., Kawazu, S., Suzuki, Y., Inoue, N.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 76 Issue: 6 Pages: 799-806

    • DOI

      10.1292/jvms.13-0462

    • NAID

      130003391280

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Diagnostic value of the recombinant tandem repeat antigen TeGM6-4r for surra in water buffaloes2014

    • Author(s)
      Nguyen TT, Zhou M, Ruttayaporn N, Nguyen QD, Nguyen VK, Goto Y, Suzuki Y, Kawazu SI, Inoue N
    • Journal Title

      Vet Parasitol

      Volume: 201 Issue: 1-2 Pages: 18-23

    • DOI

      10.1016/j.vetpar.2014.01.009

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of a fluorescence-based method for antibabesial drug screening.2014

    • Author(s)
      Guswanto A, Sivakumar T, Rizk MA, Elsayed SA, Youssef MA, ElSaid Eel S, Yokoyama N, Igarashi I.
    • Journal Title

      Antimicrob. Agents Chemother.

      Volume: 58 Issue: 8 Pages: 4713-4717

    • DOI

      10.1128/aac.00022-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] PCR detection of Babesia ovata from questing ticks in Japan.2014

    • Author(s)
      Sivakumar T, Tattiyapong M, Okubo K, Suganuma K, Hayashida K, Igarashi I, Zakimi S, Matsumoto K, Inokuma H, Yokoyama N.
    • Journal Title

      Ticks Tick Borne Dis.

      Volume: 5 Issue: 3 Pages: 305-310

    • DOI

      10.1016/j.ttbdis.2013.12.006

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Expression, immunolocalization and serodiagnostic value of Tc38630 protein from Trypanosoma congolense2013

    • Author(s)
      Mochabo, K. M., Zhou, M., Suganuma, K., Kawazu, S., Suzuki, Y. and Inoue, N.
    • Journal Title

      Parasitology Research

      Volume: 112 Issue: 9 Pages: 3357-3363

    • DOI

      10.1007/s00436-013-3515-z

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence and genetic diversity of equine piroplasms in Tov province, Mongolia.2013

    • Author(s)
      Munkhjargal T, Sivakumar T, Battsetseg B, Nyamjargal T, Aboulaila M, et al.
    • Journal Title

      Infection, Genetics and Evolution

      Volume: 16 Pages: 178-185

    • DOI

      10.1016/j.meegid.2013.02.005

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A PCR based survey of Babesia ovata in cattle from various Asian, African and South American countries.2013

    • Author(s)
      Yoshinari T, Sivakumar T, Asada M, Battsetseg B, Huang X, Lan DT, Inpankaew T, Ybañez AP, Alhassan A, Thekisoe OM, De Macedo AC, Inokuma H, Igarashi I, Yokoyama N.
    • Journal Title

      J Vet Med Sci.

      Volume: 75 Pages: 211-214

    • NAID

      130001879670

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel dense granule protein, GRA22, is involved in regulating parasite egress in Toxoplasma gondii.2013

    • Author(s)
      Okada T, Marmansari D, Li ZM, Adilbish A, Canko S, Ueno A, Shono H, Furuoka H, Igarashi M.
    • Journal Title

      Mol Biochem Parasitol.

      Volume: 189 Issue: 1-2 Pages: 5-13

    • DOI

      10.1016/j.molbiopara.2013.04.005

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Analysis of theRoleof Toxoplasma gondiiLactate dehydrogenase during stage conversion using Knockout techniques.2016

    • Author(s)
      Abdelbaset Abdelbaset、五十嵐 慎
    • Organizer
      第85回日本寄生虫学会大会
    • Place of Presentation
      宮崎市民プラザ(宮崎県・宮崎市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] トキソプラズマのシスト形成に寄与する因子の探索2016

    • Author(s)
      村田 優穂、高野 量、五十嵐 慎、加藤 健太郎
    • Organizer
      第85回日本寄生虫学会大会
    • Place of Presentation
      宮崎市民プラザ(宮崎県・宮崎市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] PCRdetectionandphylogeneticcharacterizationofBabesia microtiinquestingticksin Mongolia2015

    • Author(s)
      BumduurenTuvshintulga、SivakumarThillaiampalam、横山 直明、五十嵐 郁男
    • Organizer
      第158回日本獣医学会学術集会
    • Place of Presentation
      北里大学獣医学部(青森県・十和田市)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] A TeGM6-4r antigen based immunochromatographic test (ICT) for diagnosis of animal trypanosomosis2014

    • Author(s)
      Thu-Thuy Nguyen, Ngasaman Ruttayaporn, Yasuyuki Goto, Zhao-Rong Lun, Shin-ichiro Kawazu, Charles P. Othim, Heriel A. Mbwambo, Chihiro Sugimoto and Noboru Inoue
    • Organizer
      18th Japanese-German Cooperative Symposium on Protozoan Diseases
    • Place of Presentation
      Technische Universitat, dresden
    • Year and Date
      2014-09-30 – 2014-10-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] Prevalence and genetic characterization of Toxoplasma gondii isolate from Tokachi subprefecture in Japan.2014

    • Author(s)
      Salman Doaa、大橋英二、Abdelbaset Abdelbaset E、五十嵐慎
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Genetic diversity of Theileria2014

    • Author(s)
      横山直明、Sivakumar Thillaiamapalam、林田京子、杉本千尋
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Babesia ovata:Epidemiology and clinical significance2014

    • Author(s)
      横山直明、Sivakumar Thillaiampalam、五十嵐郁男、猪熊壽
    • Organizer
      第83回日本寄生虫学会大会
    • Place of Presentation
      愛媛大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification and Characterization of novel bradyzoite-specific genes in Toxoplasma gondii2013

    • Author(s)
      Doaa Salman、Muller Compaore、Suphasawatt Puranaveja、岡田只士、五十嵐慎
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜大学
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2022-02-01  

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