Epidemiological and experimental studies of Tick-borne viruses in Indochina

• Project/Area Number: 25304045
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Veterinary medical science
• Research Institution: Nagasaki University
• Principal Investigator: HAYASAKA Daisuke 長崎大学, 熱帯医学研究所, 助教 (10346926)
• Co-Investigator(Kenkyū-buntansha): NABESHIMA Takeshi 長崎大学, 熱帯医学研究所, 助教 (30546859) ; KUROSAKI Yohei 長崎大学, 熱帯医学研究所, 助教 (40415443) ; HOTTA Kozue 東京大学, 大学院農学生命科学研究科(農学部), 助教 (70466192) ; TSUNODA Takashi 長崎大学, 熱帯医学研究所, 助教 (30457527)
• Co-Investigator(Renkei-kenkyūsha): KATO Kentaro 長崎大学, 熱帯医学研究所, 助教 (50508885) ; YAHATA Kazuhide 長崎大学, 熱帯医学研究所, 助教 (40467965) ; INOUE Shingo 長崎大学, 熱帯医学研究所, 助教 (00346925)
• Research Collaborator: SHIMADA Satoshi ; YU Fuxun ; AOKI Kotaro ; MYA Myat Ngwe Tun ; UCHIDA Leo ; TAKAMATSU Yuki ; GUILLERMO Posadas-Herrera ; SAKAGUCHI Miako ; YASUDA Jiro ; FUJITA Hiromi ; MORITA Kouichi
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: マダニ媒介ウイルス / ベトナム / マダニ / インドシナ地域 / 病原性 / SFTSウイルス / Toflaウイルス / リケッチア / マダニ媒介感染症 / ナイロウイルス / ウイルス / カンボジア / SFTS

Outline of Final Research Achievements

In this study, we investigated the distributions of tick species in the different geographical areas of Indochina and Japan, detected tick-borne viruses from ticks, developed serodiagnosis and viral gene detection methods for tick-borne viruses, and analyzed of infectivity and pathogenicity of tick-borne viruses in vivo and in vitro. The results from this study will provide useful information for prevention of tick-borne viral diseases in affected areas including Indochina and Japan.

Research Products

• [Int'l Joint Research] NIHE(ベトナム)
• [Int'l Joint Research] Henan CDC(China)
• [Int'l Joint Research] Savannakhet Provincial Malaria Station(ラオス)
• [Journal Article] Severe Fever with Thrombocytopenia Syndrome Virus antibody investigation among domestic bovine transported to slaughterhouse in Shimane Prefecture, Japan (2016)
  • Author(s): Kenji Tabara, Hiromi Fujita, Atau Hirata, Daisuke Hayasaka
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 印刷中
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Seroepidemiological evidence of severe fever with thrombocytopenia syndrome virus infections in wild boars in Nagasaki, Japan (2016)
  • Author(s): Daisuke Hayasaka, Yu Fuxun, Akira Yoshikawa, Guillermo Posadas-Herrera, Satoshi Shimada, Ngwe Tun Mya Myat, Masanobu Agoh, Kouichi Morita
  • Journal Title: Tropical Medicine and Health Volume: 44:6 Issue: 1 Pages: 1-5
  • DOI: 10.1186/s41182-016-0009-6
  • NAID: 120006987653
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Tofla virus: A newly identified Nairovirus of the Crimean-Congo hemorrhagic fever group isolated from ticks in Japan. (2016)
  • Author(s): Shimada S, Aoki K, Nabeshima T, Fuxun Y, Kurosaki Y, Shiogama K, Onouchi T, Sakaguchi M, Fuchigami T, Ono H, Nishi K, Posadas-Herrera G, Uchida L, Takamatsu Y, Yasuda J, Tsutsumi Y, Fujita H, Morita K, Hayasaka D.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 20213-20213
  • DOI: 10.1038/srep20213
  • NAID: 120006666534
  • Peer Reviewed / Open Access
• [Journal Article] Prevalence and phylogenetic analysis of Orientia tsutsugamushi in small mammals in Hanoi, Vietnam (2016)
  • Author(s): Hotta Kozue, Pham Hang T.T., Hoang Huong T., Trang Tu C., Vu Thuy N., Ung Trang T.H., Shimizu Kenta, Arikawa Jiro, Yamada Akio, Nguyen Hoa T., Nguyen Hang L.K., Le Mai T.Q., Daisuke Hayasaka
  • Journal Title: Vector-Borne and Zoonotic Diseases Volume: 16(2) Issue: 2 Pages: 96-102
  • DOI: 10.1089/vbz.2015.1831
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 18F-FDG PET imaging for identifying the dynamics of intestinal disease caused by SFTSV infection in a mouse model. (2016)
  • Author(s): Hayasaka D, Nishi K, Fuchigami T, Shiogama K, Onouchi T, Shimada S, Tsutsumi Y, Morita K.
  • Journal Title: Oncotarget Volume: 7 Issue: 1 Pages: 140-147
  • DOI: 10.18632/oncotarget.6645
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] NS1’ protein expression in the JaOArS982 strain of Japanese encephalitis virus does not enhance virulence in mice (2015)
  • Author(s): Yuki Takamatsu, Muhareva Raekiansyah, Kouichi Morita, Daisuke Hayasaka
  • Journal Title: Tropical Medicine and Health Volume: 43(4) Issue: 4 Pages: 233-237
  • DOI: 10.2149/tmh.2015-27
  • NAID: 130005130388
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Coated Glass Slides TACAS Are Applicable to Heat-Assisted Immunostaining and <i>In Situ</i> Hybridization at the Electron Microscopy Level (2015)
  • Author(s): Matsui T, Onouchi T, Shiogama K, Mizutani Y, Inada K, Yu F, Hayasaka D, Morita K, Ogawa H, Mahara F, Tsutsumi Y.
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 48 Issue: 5 Pages: 153-157
  • DOI: 10.1267/ahc.15012
  • NAID: 130005106598
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Epidemiological Survey of Severe Fever with Thrombocytopenia Syndrome Virus in Ticks in Nagasaki, Japan (2015)
  • Author(s): Daisuke Hayasaka, Satoshi Shimada, Kotaro Aoki, Yuki Takamatsu, Leo Uchida, Masahiro Horio, Yu Fuxun, Kouichi Morita
  • Journal Title: Tropical Medicine and Health Volume: 43 Issue: 3 Pages: 159-164
  • DOI: 10.2149/tmh.2015-01
  • NAID: 130005102104
  • ISSN: 1348-8945, 1349-4147
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Application of recombinant severe fever with thrombocytopenia syndrome virus nucleocapsid protein for the detection of SFTSV-specific human IgG and IgM antibodies by indirect ELISA (2015)
  • Author(s): Fuxun Yu, Yanhua Du, Xueyong Huang, Hong Ma, Bianli Xu, Ferdinard Adungo, Daisuke Hayasaka, Corazon C. Buerano, Kouichi Morita
  • Journal Title: Virology Journal Volume: 12:117 Issue: 1 Pages: 1-7
  • DOI: 10.1186/s12985-015-0350-0
  • NAID: 120006986976
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A unique amino acid substitution in NS2A protein of Japanese encephalitis virus affects virus propagation in vitro but not in vivo (2015)
  • Author(s): Yuki Takamatsu, Kouichi Morita, Daisuke Hayasaka
  • Journal Title: Journal of Virology Volume: 89(11) Issue: 11 Pages: 6126-6130
  • DOI: 10.1128/jvi.00370-15
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Therapeutic effect of post-exposure treatment with antiserum on severe fever with thrombocytopenia syndrome (SFTS) in a mouse model of SFTS virus infection (2015)
  • Author(s): Satoshi Shimada, Guillermo Posadas-Herrera , Kotaro Aoki, Kouichi Morita, Daisuke Hayasaka
  • Journal Title: Virology Volume: 482 Pages: 19-27
  • DOI: 10.1016/j.virol.2015.03.010
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Protective role of TNF-α, IL-10 and IL-2 in mice infected with the Oshima strain of Tick-borne encephalitis virus. (2014)
  • Author(s): Tun MM, Aoki K, Senba M, Buerano CC, Shirai K, Suzuki R, Morita K, Hayasaka D.
  • Journal Title: Sci Rep Volume: 4 Issue: 1 Pages: 5344-5344
  • DOI: 10.1038/srep05344
  • NAID: 120006986532
  • Peer Reviewed / Open Access
• [Journal Article] Type-I interferon response affects an inoculation dose-independent mortality in mice following Japanese encephalitis virus infection (2014)
  • Author(s): Kotaro Aoki, Satoshi Shimada, Dash Sima Simantini, Mya Myat Ngwe Tun, Corazon C Buerano, Kouichi Morita, Daisuke Hayasaka
  • Journal Title: Virology Journal Volume: 11:105 Issue: 1 Pages: 1-7
  • DOI: 10.1186/1743-422x-11-105
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Pathologic Potential of Variant Clones of the Oshima Strain of Far-Eastern Subtype Tick-Borne Encephalitis Virus (2014)
  • Author(s): Le Xuan Luat, Mya Myat Ngwe Tun, Corazon C. Buerano, Kotaro Aoki, Kouichi Morita, Daisuke Hayasaka
  • Journal Title: Tropical Medicine and Health Volume: 42 Issue: 1 Pages: 15-23
  • DOI: 10.2149/tmh.2013-27
  • NAID: 130003392440
  • ISSN: 1348-8945, 1349-4147
  • Peer Reviewed
• [Presentation] 18F-FDG PET imaging for identifying the dynamics of intestinal disease caused by SFTSV infection in a mouse model (2016)
  • Author(s): Daisuke Hayasaka
  • Organizer: The U.S Japan Cooperative Medical Sciences Program presents 50th Anniversary Celebration followed by the 18th International Conference on Emerging Infectious Diseases (EID)
  • Place of Presentation: Bethesda North Marriott Hotel and Conference Center, Bethesda, USA
  • Year and Date: 2016-01-13
  • Int'l Joint Research
• [Presentation] Tofla virus: a new Nairovirus belonging to the Crimean-Congo hemorrhagic fever group isolated from ticks in Japan (2015)
  • Author(s): Satoshi Shimada，Kotaro Aoki，Takeshi Nabeshima，YuFuxun，Yohei Kurosaki，Kazuya Shiogama，Takanori Onouchi，Miako Sakaguchi，Posadas-Herrera Guillermo，Leo Uchida，Yuki Takamatsu，Jiro Yasuda，Yutaka Tsutsumi，Hiromi Fujita，Kouichi Morita，Daisuke Hayasaka
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡国際会議場、福岡市
  • Year and Date: 2015-11-22
• [Presentation] 18F-FDG PET imaging for identifying the dynamics of gastroenteritis in a mouse model of SFTSV infection (2015)
  • Author(s): Daisuke Hayasaka, Kodai Nishi, Takeshi Fuchigami, Kazuya Shiogama, Takanori Onouchi, Satoshi Shimada, Yutaka Tsutsumi, Kouichi Morita
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡国際会議場、福岡市
  • Year and Date: 2015-11-22
• [Presentation] 国内のマダニから分離された新規ナイロウイルス (2015)
  • Author(s): 嶋田聡、青木康太郎、鍋島武、余福勲、坂口美亜子、森田公一、早坂大輔
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学獣医学部、十和田市
  • Year and Date: 2015-09-08
• [Presentation] 国内のマダニから分離された新規ナイロウイルス (2015)
  • Author(s): 嶋田聡、青木康太郎、鍋島武、余福勲、黒崎陽平、塩竈和也、尾之内高慶、坂口美亜子、淵上剛、小野北斗、西弘大、Guillermo Posadas Herrera、内田玲麻、高松由基、安田二朗、堤寛、藤田博己、森田公一、早坂大輔
  • Organizer: 第52回ウイルス学会九州支部総会
  • Place of Presentation: 別府ビーコンプラザ、別府市
  • Year and Date: 2015-09-04
• [Presentation] 国内のマダニから分離された新規ナイロウイルス (2015)
  • Author(s): 早坂大輔
  • Organizer: 第23回Seminar on Acari-Disease Interface
  • Place of Presentation: 名取市文化会館、名取市
  • Year and Date: 2015-06-27
• [Presentation] 重症熱性血小板減少症候群ウイルス（SFTS）感染マウスモデルを用いた抗血清および薬剤効果の検討 (2015)
  • Author(s): 嶋田聡、Guillermo Posadas Herrera、青木康太郎、森田公一、早坂大輔
  • Organizer: 第50回日本脳炎ウイルス生態学研究会
  • Place of Presentation: ホテル京都エミナース、京都市
  • Year and Date: 2015-05-16
• [Presentation] 国内のマダニから分離された新規ナイロウイルス (2015)
  • Author(s): 嶋田聡、青木康太郎、鍋島武、余福勲、黒崎陽平、塩竈和也、尾之内高慶、坂口美亜子、淵上剛、小野北斗、西弘大、Guillermo Posadas Herrera、内田玲麻、高松由基、安田二朗、堤寛、藤田博己、森田公一、早坂大輔
  • Organizer: 第50回日本脳炎ウイルス生態学研究会
  • Place of Presentation: ホテル京都エミナース、京都市
  • Year and Date: 2015-05-16
• [Presentation] 山口県のイノシシからLangatウイルスに対する抗体の検出 (2015)
  • Author(s): 下田宙、早坂大輔、好井健太朗、米満研三、鍬田龍星、高野愛、前田健
  • Organizer: 第50回日本脳炎ウイルス生態学研究会
  • Place of Presentation: ホテル京都エミナース、京都市
  • Year and Date: 2015-05-15
• [Presentation] 長崎県における野生動物およびマダニのSFTSウイルス感染状況の調査 (2014)
  • Author(s): 早坂大輔、余福勲、吉川亮、嶋田聡、Guillermo Posadas Herrera 、Mya Myat Ngwe Tun,吾郷昌信、森田公一
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: 神奈川県、横浜市、パシフィコ横浜
  • Year and Date: 2014-11-11
• [Presentation] 重症熱性血小板減少症候群(SFTS)ウイルス遺伝子検出法の確立と長崎県における媒介マダニ調査 (2014)
  • Author(s): 早坂大輔、嶋田聡、青木康太郎、森田公一
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道、札幌市、北海道大学
  • Year and Date: 2014-09-10
• [Presentation] 新規ナイロウイルス遺伝子検出法の開発 (2014)
  • Author(s): 黒崎陽平、中前小百合、早坂大輔、安田二朗
  • Organizer: 第51回ウイルス学会九州支部総会
  • Place of Presentation: 鹿児島県、鹿児島市、城山観光ホテル
  • Year and Date: 2014-09-05
• [Presentation] 重症熱性血小板減少症候群(SFTS)ウイルス感染マウスモデルを用いた抗血清および薬剤効果の検討 (2014)
  • Author(s): 早坂大輔、嶋田聡、Guillermo Posadas Herrera、森田公一
  • Organizer: 第51回ウイルス学会九州支部総会
  • Place of Presentation: 鹿児島県、鹿児島市、城山観光ホテル
  • Year and Date: 2014-09-05
• [Presentation] SFTSウイルスについてこれまでわかったこと (2014)
  • Author(s): 早坂大輔
  • Organizer: 第22回Seminar on Acari-Disease Interface
  • Place of Presentation: 福岡県、太宰府市、太宰府館まほろばホール
  • Year and Date: 2014-07-05
• [Presentation] 山口県のイノシシからダニ媒介性脳炎ウイルス様遺伝子の検出
  • Author(s): 下田 宙、早坂大輔、好井健太朗、米満研三、寺田 豊、野口慧多、 鍬田龍星、高野 愛、前田 健
  • Organizer: 第20回トガ・フラビ・ペスチウイルス研究会
  • Place of Presentation: 神戸市、神戸国際会議場、兵庫県
• [Presentation] 重症熱性血小板減少症候群(SFTS)の血清診断法の確立および媒介マダニ調査
  • Author(s): 早坂 大輔、森田 公一
  • Organizer: 第45回九州微生物研究会総会
  • Place of Presentation: 福岡市、ホテルセントラーザ博多、福岡県
• [Presentation] 長崎県における重症熱性血小板減少症候群(SFTS)の動物血清疫学および媒介マダニ調査
  • Author(s): 早坂 大輔、余 福勲、吉川 亮、Guillermo Posadas Herrera、Mya Myat Ngwe Tun、吾郷 昌信、森田 公一
  • Organizer: 第38回長崎感染症研究会
  • Place of Presentation: 長崎市、長崎大学、長崎県