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Epidemiological and experimental studies of Tick-borne viruses in Indochina

Research Project

Project/Area Number 25304045
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section海外学術
Research Field Veterinary medical science
Research InstitutionNagasaki University

Principal Investigator

HAYASAKA Daisuke  長崎大学, 熱帯医学研究所, 助教 (10346926)

Co-Investigator(Kenkyū-buntansha) NABESHIMA Takeshi  長崎大学, 熱帯医学研究所, 助教 (30546859)
KUROSAKI Yohei  長崎大学, 熱帯医学研究所, 助教 (40415443)
HOTTA Kozue  東京大学, 大学院農学生命科学研究科(農学部), 助教 (70466192)
TSUNODA Takashi  長崎大学, 熱帯医学研究所, 助教 (30457527)
Co-Investigator(Renkei-kenkyūsha) KATO Kentaro  長崎大学, 熱帯医学研究所, 助教 (50508885)
YAHATA Kazuhide  長崎大学, 熱帯医学研究所, 助教 (40467965)
INOUE Shingo  長崎大学, 熱帯医学研究所, 助教 (00346925)
Research Collaborator SHIMADA Satoshi  
YU Fuxun  
AOKI Kotaro  
MYA Myat Ngwe Tun  
UCHIDA Leo  
TAKAMATSU Yuki  
GUILLERMO Posadas-Herrera  
SAKAGUCHI Miako  
YASUDA Jiro  
FUJITA Hiromi  
MORITA Kouichi  
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywordsマダニ媒介ウイルス / ベトナム / マダニ / インドシナ地域 / 病原性 / SFTSウイルス / Toflaウイルス / リケッチア / マダニ媒介感染症 / ナイロウイルス / ウイルス / カンボジア / SFTS
Outline of Final Research Achievements

In this study, we investigated the distributions of tick species in the different geographical areas of Indochina and Japan, detected tick-borne viruses from ticks, developed serodiagnosis and viral gene detection methods for tick-borne viruses, and analyzed of infectivity and pathogenicity of tick-borne viruses in vivo and in vitro. The results from this study will provide useful information for prevention of tick-borne viral diseases in affected areas including Indochina and Japan.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (34 results)

All 2016 2015 2014 Other

All Int'l Joint Research (3 results) Journal Article (14 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 14 results,  Open Access: 10 results,  Acknowledgement Compliant: 9 results) Presentation (17 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] NIHE(ベトナム)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Henan CDC(China)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Savannakhet Provincial Malaria Station(ラオス)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Severe Fever with Thrombocytopenia Syndrome Virus antibody investigation among domestic bovine transported to slaughterhouse in Shimane Prefecture, Japan2016

    • Author(s)
      Kenji Tabara, Hiromi Fujita, Atau Hirata, Daisuke Hayasaka
    • Journal Title

      Japanese Journal of Infectious Diseases

      Volume: 印刷中

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Seroepidemiological evidence of severe fever with thrombocytopenia syndrome virus infections in wild boars in Nagasaki, Japan2016

    • Author(s)
      Daisuke Hayasaka, Yu Fuxun, Akira Yoshikawa, Guillermo Posadas-Herrera, Satoshi Shimada, Ngwe Tun Mya Myat, Masanobu Agoh, Kouichi Morita
    • Journal Title

      Tropical Medicine and Health

      Volume: 44:6 Issue: 1 Pages: 1-5

    • DOI

      10.1186/s41182-016-0009-6

    • NAID

      120006987653

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Tofla virus: A newly identified Nairovirus of the Crimean-Congo hemorrhagic fever group isolated from ticks in Japan.2016

    • Author(s)
      Shimada S, Aoki K, Nabeshima T, Fuxun Y, Kurosaki Y, Shiogama K, Onouchi T, Sakaguchi M, Fuchigami T, Ono H, Nishi K, Posadas-Herrera G, Uchida L, Takamatsu Y, Yasuda J, Tsutsumi Y, Fujita H, Morita K, Hayasaka D.
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 20213-20213

    • DOI

      10.1038/srep20213

    • NAID

      120006666534

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prevalence and phylogenetic analysis of Orientia tsutsugamushi in small mammals in Hanoi, Vietnam2016

    • Author(s)
      Hotta Kozue, Pham Hang T.T., Hoang Huong T., Trang Tu C., Vu Thuy N., Ung Trang T.H., Shimizu Kenta, Arikawa Jiro, Yamada Akio, Nguyen Hoa T., Nguyen Hang L.K., Le Mai T.Q., Daisuke Hayasaka
    • Journal Title

      Vector-Borne and Zoonotic Diseases

      Volume: 16(2) Issue: 2 Pages: 96-102

    • DOI

      10.1089/vbz.2015.1831

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 18F-FDG PET imaging for identifying the dynamics of intestinal disease caused by SFTSV infection in a mouse model.2016

    • Author(s)
      Hayasaka D, Nishi K, Fuchigami T, Shiogama K, Onouchi T, Shimada S, Tsutsumi Y, Morita K.
    • Journal Title

      Oncotarget

      Volume: 7 Issue: 1 Pages: 140-147

    • DOI

      10.18632/oncotarget.6645

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] NS1’ protein expression in the JaOArS982 strain of Japanese encephalitis virus does not enhance virulence in mice2015

    • Author(s)
      Yuki Takamatsu, Muhareva Raekiansyah, Kouichi Morita, Daisuke Hayasaka
    • Journal Title

      Tropical Medicine and Health

      Volume: 43(4) Issue: 4 Pages: 233-237

    • DOI

      10.2149/tmh.2015-27

    • NAID

      130005130388

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Coated Glass Slides TACAS Are Applicable to Heat-Assisted Immunostaining and <i>In Situ</i> Hybridization at the Electron Microscopy Level2015

    • Author(s)
      Matsui T, Onouchi T, Shiogama K, Mizutani Y, Inada K, Yu F, Hayasaka D, Morita K, Ogawa H, Mahara F, Tsutsumi Y.
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 48 Issue: 5 Pages: 153-157

    • DOI

      10.1267/ahc.15012

    • NAID

      130005106598

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Epidemiological Survey of Severe Fever with Thrombocytopenia Syndrome Virus in Ticks in Nagasaki, Japan2015

    • Author(s)
      Daisuke Hayasaka, Satoshi Shimada, Kotaro Aoki, Yuki Takamatsu, Leo Uchida, Masahiro Horio, Yu Fuxun, Kouichi Morita
    • Journal Title

      Tropical Medicine and Health

      Volume: 43 Issue: 3 Pages: 159-164

    • DOI

      10.2149/tmh.2015-01

    • NAID

      130005102104

    • ISSN
      1348-8945, 1349-4147
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Application of recombinant severe fever with thrombocytopenia syndrome virus nucleocapsid protein for the detection of SFTSV-specific human IgG and IgM antibodies by indirect ELISA2015

    • Author(s)
      Fuxun Yu, Yanhua Du, Xueyong Huang, Hong Ma, Bianli Xu, Ferdinard Adungo, Daisuke Hayasaka, Corazon C. Buerano, Kouichi Morita
    • Journal Title

      Virology Journal

      Volume: 12:117 Issue: 1 Pages: 1-7

    • DOI

      10.1186/s12985-015-0350-0

    • NAID

      120006986976

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A unique amino acid substitution in NS2A protein of Japanese encephalitis virus affects virus propagation in vitro but not in vivo2015

    • Author(s)
      Yuki Takamatsu, Kouichi Morita, Daisuke Hayasaka
    • Journal Title

      Journal of Virology

      Volume: 89(11) Issue: 11 Pages: 6126-6130

    • DOI

      10.1128/jvi.00370-15

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Therapeutic effect of post-exposure treatment with antiserum on severe fever with thrombocytopenia syndrome (SFTS) in a mouse model of SFTS virus infection2015

    • Author(s)
      Satoshi Shimada, Guillermo Posadas-Herrera , Kotaro Aoki, Kouichi Morita, Daisuke Hayasaka
    • Journal Title

      Virology

      Volume: 482 Pages: 19-27

    • DOI

      10.1016/j.virol.2015.03.010

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Protective role of TNF-α, IL-10 and IL-2 in mice infected with the Oshima strain of Tick-borne encephalitis virus.2014

    • Author(s)
      Tun MM, Aoki K, Senba M, Buerano CC, Shirai K, Suzuki R, Morita K, Hayasaka D.
    • Journal Title

      Sci Rep

      Volume: 4 Issue: 1 Pages: 5344-5344

    • DOI

      10.1038/srep05344

    • NAID

      120006986532

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Type-I interferon response affects an inoculation dose-independent mortality in mice following Japanese encephalitis virus infection2014

    • Author(s)
      Kotaro Aoki, Satoshi Shimada, Dash Sima Simantini, Mya Myat Ngwe Tun, Corazon C Buerano, Kouichi Morita, Daisuke Hayasaka
    • Journal Title

      Virology Journal

      Volume: 11:105 Issue: 1 Pages: 1-7

    • DOI

      10.1186/1743-422x-11-105

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Pathologic Potential of Variant Clones of the Oshima Strain of Far-Eastern Subtype Tick-Borne Encephalitis Virus2014

    • Author(s)
      Le Xuan Luat, Mya Myat Ngwe Tun, Corazon C. Buerano, Kotaro Aoki, Kouichi Morita, Daisuke Hayasaka
    • Journal Title

      Tropical Medicine and Health

      Volume: 42 Issue: 1 Pages: 15-23

    • DOI

      10.2149/tmh.2013-27

    • NAID

      130003392440

    • ISSN
      1348-8945, 1349-4147
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 18F-FDG PET imaging for identifying the dynamics of intestinal disease caused by SFTSV infection in a mouse model2016

    • Author(s)
      Daisuke Hayasaka
    • Organizer
      The U.S Japan Cooperative Medical Sciences Program presents 50th Anniversary Celebration followed by the 18th International Conference on Emerging Infectious Diseases (EID)
    • Place of Presentation
      Bethesda North Marriott Hotel and Conference Center, Bethesda, USA
    • Year and Date
      2016-01-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Tofla virus: a new Nairovirus belonging to the Crimean-Congo hemorrhagic fever group isolated from ticks in Japan2015

    • Author(s)
      Satoshi Shimada,Kotaro Aoki,Takeshi Nabeshima,YuFuxun,Yohei Kurosaki,Kazuya Shiogama,Takanori Onouchi,Miako Sakaguchi,Posadas-Herrera Guillermo,Leo Uchida,Yuki Takamatsu,Jiro Yasuda,Yutaka Tsutsumi,Hiromi Fujita,Kouichi Morita,Daisuke Hayasaka
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡国際会議場、福岡市
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 18F-FDG PET imaging for identifying the dynamics of gastroenteritis in a mouse model of SFTSV infection2015

    • Author(s)
      Daisuke Hayasaka, Kodai Nishi, Takeshi Fuchigami, Kazuya Shiogama, Takanori Onouchi, Satoshi Shimada, Yutaka Tsutsumi, Kouichi Morita
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡国際会議場、福岡市
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 国内のマダニから分離された新規ナイロウイルス2015

    • Author(s)
      嶋田聡、青木康太郎、鍋島武、余福勲、坂口美亜子、森田公一、早坂大輔
    • Organizer
      第158回日本獣医学会学術集会
    • Place of Presentation
      北里大学獣医学部、十和田市
    • Year and Date
      2015-09-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] 国内のマダニから分離された新規ナイロウイルス2015

    • Author(s)
      嶋田聡、青木康太郎、鍋島武、余福勲、黒崎陽平、塩竈和也、尾之内高慶、坂口美亜子、淵上剛、小野北斗、西弘大、Guillermo Posadas Herrera、内田玲麻、高松由基、安田二朗、堤寛、藤田博己、森田公一、早坂大輔
    • Organizer
      第52回ウイルス学会九州支部総会
    • Place of Presentation
      別府ビーコンプラザ、別府市
    • Year and Date
      2015-09-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] 国内のマダニから分離された新規ナイロウイルス2015

    • Author(s)
      早坂大輔
    • Organizer
      第23回Seminar on Acari-Disease Interface
    • Place of Presentation
      名取市文化会館、名取市
    • Year and Date
      2015-06-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 重症熱性血小板減少症候群ウイルス(SFTS)感染マウスモデルを用いた抗血清および薬剤効果の検討2015

    • Author(s)
      嶋田聡、Guillermo Posadas Herrera、青木康太郎、森田公一、早坂大輔
    • Organizer
      第50回日本脳炎ウイルス生態学研究会
    • Place of Presentation
      ホテル京都エミナース、京都市
    • Year and Date
      2015-05-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 国内のマダニから分離された新規ナイロウイルス2015

    • Author(s)
      嶋田聡、青木康太郎、鍋島武、余福勲、黒崎陽平、塩竈和也、尾之内高慶、坂口美亜子、淵上剛、小野北斗、西弘大、Guillermo Posadas Herrera、内田玲麻、高松由基、安田二朗、堤寛、藤田博己、森田公一、早坂大輔
    • Organizer
      第50回日本脳炎ウイルス生態学研究会
    • Place of Presentation
      ホテル京都エミナース、京都市
    • Year and Date
      2015-05-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 山口県のイノシシからLangatウイルスに対する抗体の検出2015

    • Author(s)
      下田宙、早坂大輔、好井健太朗、米満研三、鍬田龍星、高野愛、前田健
    • Organizer
      第50回日本脳炎ウイルス生態学研究会
    • Place of Presentation
      ホテル京都エミナース、京都市
    • Year and Date
      2015-05-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] 長崎県における野生動物およびマダニのSFTSウイルス感染状況の調査2014

    • Author(s)
      早坂大輔、余福勲、吉川亮、嶋田聡、Guillermo Posadas Herrera 、Mya Myat Ngwe Tun,吾郷昌信、森田公一
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      神奈川県、横浜市、パシフィコ横浜
    • Year and Date
      2014-11-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 重症熱性血小板減少症候群(SFTS)ウイルス遺伝子検出法の確立と長崎県における媒介マダニ調査2014

    • Author(s)
      早坂大輔、嶋田聡、青木康太郎、森田公一
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道、札幌市、北海道大学
    • Year and Date
      2014-09-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 新規ナイロウイルス遺伝子検出法の開発2014

    • Author(s)
      黒崎陽平、中前小百合、早坂大輔、安田二朗
    • Organizer
      第51回ウイルス学会九州支部総会
    • Place of Presentation
      鹿児島県、鹿児島市、城山観光ホテル
    • Year and Date
      2014-09-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 重症熱性血小板減少症候群(SFTS)ウイルス感染マウスモデルを用いた抗血清および薬剤効果の検討2014

    • Author(s)
      早坂大輔、嶋田聡、Guillermo Posadas Herrera、森田公一
    • Organizer
      第51回ウイルス学会九州支部総会
    • Place of Presentation
      鹿児島県、鹿児島市、城山観光ホテル
    • Year and Date
      2014-09-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] SFTSウイルスについてこれまでわかったこと2014

    • Author(s)
      早坂大輔
    • Organizer
      第22回Seminar on Acari-Disease Interface
    • Place of Presentation
      福岡県、太宰府市、太宰府館まほろばホール
    • Year and Date
      2014-07-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 山口県のイノシシからダニ媒介性脳炎ウイルス様遺伝子の検出

    • Author(s)
      下田 宙、早坂大輔、好井健太朗、米満研三、寺田 豊、野口慧多、 鍬田龍星、高野 愛、前田 健
    • Organizer
      第20回トガ・フラビ・ペスチウイルス研究会
    • Place of Presentation
      神戸市、神戸国際会議場、兵庫県
    • Related Report
      2013 Annual Research Report
  • [Presentation] 重症熱性血小板減少症候群(SFTS)の血清診断法の確立および媒介マダニ調査

    • Author(s)
      早坂 大輔、森田 公一
    • Organizer
      第45回九州微生物研究会総会
    • Place of Presentation
      福岡市、ホテルセントラーザ博多、福岡県
    • Related Report
      2013 Annual Research Report
  • [Presentation] 長崎県における重症熱性血小板減少症候群(SFTS)の動物血清疫学および媒介マダニ調査

    • Author(s)
      早坂 大輔、余 福勲、吉川 亮、Guillermo Posadas Herrera、Mya Myat Ngwe Tun、吾郷 昌信、森田 公一
    • Organizer
      第38回長崎感染症研究会
    • Place of Presentation
      長崎市、長崎大学、長崎県
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2022-02-01  

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