| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Outline of Final Research Achievements |
Nucleopolyhedroviruses (NPVs) have been isolated from various saturniid silkmoths (wild silkworms) utilized for silk production from East to South Asian countries, and their whole genome DNA sequences have been analyzed and compared using Next-Generation sequencer. As a result, a mutiplex-PCR diagnostic protocol, which enables to detect each NPV with high sensitivity and specificificity, has been successfully developed and useful knowledge in construction of baculovirus expression vector system with advantageous properties for valuable protein production and in consideration of genetic variation and evolution of NPVs infecting wild silkworms have been obtained.
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