Genetic diversity and evolutionary history of HCV genotype 6 variants in Southeast Asia

• Project/Area Number: 25305006
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Experimental pathology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: ABE KENJI 国立感染症研究所, その他部局等, 研究員 (60130415)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: B型肝炎 / C型肝炎 / 肝癌 / ウイルス変異株 / ウイルスゲノタイプ / エマージング感染症 / ベトナム / 東南アジア / HBVゲノタイプ / HCVゲノタイプ / 麻疹流行 / C型肝炎ウイルス / HCVゲノタイプ6変異株 / 風疹 / カンボジア

Outline of Final Research Achievements

In Vietnam and Cambodia, we confirmed 3 different genotypes consists of genotypes 1, 2 and 6 were prevalent. In both countries, genotype 6 variants were most predominant (more than half), followed by genotype 1 and genotype 2. We identified multiple HCV lineages (6a/6e/6h/6k/6l/6o/6p) in Vietnam may represent the indigenous strains; 1a was probably introduced from the US; 1b and 2a possibly originated in East Asia; while 2i/2j/2m were likely brought by French explorers. Furthermore, we inferred the evolutionary history for four major subtypes (1a/1b/6a/6e). The obtained Bayesian Skyline Plots (BSPs) consistently showed the rapid HCV population growth from 1955 to 1963 until 1984 or after, corresponding to the era of theVietnam War. In addition, virological and pathological characteristics of hepatocellular carcinoma (HCC) in Vietnam and occurrence of occult B/C infection among non-B/non-C HCC patients in various countries were reported.

Research Products

• [Int'l Joint Research] ホーチミン医科薬科大学/チョーライ病院/Hung Vuong 産科病院(ベトナム)
• [Int'l Joint Research] チュラロンコン大学医学部/チュラロンコン大学獣医学部(タイ)
• [Int'l Joint Research] 保健省医学研究局・病理部門(ミャンマー)
• [Int'l Joint Research] 国立公衆衛生研究所/パスツール研究所(カンボジア)
• [Int'l Joint Research] マウントサイナイ病院病理部/カンサス大学医療センターウイルス発癌センター/スタンフォード大学病院消化器内科(米国)
• [Int'l Joint Research]
• [Journal Article] Pathogenesis of congenital rubella virus infection in human fetuses: Viral infection in the ciliary body could play an important role in cataractogenesis. (2015)
  • Author(s): Thong Van Nguyen, Van Hung Pham, Kenji Abe.
  • Journal Title: EBioMedicine Volume: 2 Issue: 1 Pages: 59-63
  • DOI: 10.1016/j.ebiom.2014.10.021
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] An adolescent female having hepatocellular carcinoma associated with hepatitis B virus genotype H with a deletion mutation in the pre-S2 region. (2015)
  • Author(s): Oba U, Koga Y, Hoshina T, Suminoe A, Abe K, Hayashida M, Taguchi T, Hara T.
  • Journal Title: J Infect Chemother Volume: 21(4) Issue: 4 Pages: 302-304
  • DOI: 10.1016/j.jiac.2014.10.015
  • Peer Reviewed
• [Journal Article] Support of the infectivity of hepatitis delta virus particles by the envelope proteins of different genotypes of hepatitis B virus. (2014)
  • Author(s): Natalia Freitas, Kenji Abe, Celso Cunha, Stephan Menne, Severin O. Gudima:
  • Journal Title: Journal of Virology Volume: 88 Issue: 11 Pages: 6255-6267
  • DOI: 10.1128/jvi.00346-14
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Novel quasi-subgenotype D2 of hepatitis B virus identified in Taiwanese aborigines. (2014)
  • Author(s): Huy Tran, Ming-Lung Yu, Chia-Yen Dai, I-Ling Lin, Ming-Lun Yeh, Wan-Long Chuang, Kenji Abe:
  • Journal Title: Genes Volume: 49 Issue: 1 Pages: 30-37
  • DOI: 10.1007/s11262-014-1072-x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The genetic diversity and evolutionary history of hepatitis C virus in Vietnam. (2014)
  • Author(s): Chunhua Li, ManqiongYuan, Ling Lu, Teng Lu, Wenjie Xia, Van H. Pham, An X.D. Vo, Mindie H. Nguyen, Kenji Abe:
  • Journal Title: Virology Volume: 468-470 Pages: 197-206
  • DOI: 10.1016/j.virol.2014.07.026
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Nine additional complete genome sequences of HCV genotype 6 from Vietnam including new subtypes 6xb and 6xc. (2014)
  • Author(s): Chunhua Li, Van H. Pham, Kenji Abe, Ling Lu:
  • Journal Title: Virology Volume: 468-470 Pages: 172-177
  • DOI: 10.1016/j.virol.2014.08.006
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Measles epidemics among children in Vietnam: Genomic characterization of virus responsible for measles outbreak in Ho Chi Minh City, 2014. (2014)
  • Author(s): Van H. Pham, Diem P.H. Nguyet, Khanh N.H. Mai, Khanh H. Truong, Loc V. Huynh, Trang H.T. Pham, Kenji Abe:
  • Journal Title: EBioMedicine Volume: 1 Issue: 2-3 Pages: 133-140
  • DOI: 10.1016/j.ebiom.2014.10.015
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Eight novel hepatitis C virus genomes reveal the changing taxonomic structure of genotype 6. (2013)
  • Author(s): Wang H, Yuan Z, Barnes E, Yuan M, Li C, Fu Y, Xia X, Li G, Newton P, Vongsouvath M, Klenerman P, Pybus OG, Murphy D, Abe K, Lu L
  • Journal Title: Journal of General Virology Volume: 94 Issue: 1 Pages: 76-80
  • DOI: 10.1099/vir.0.047506-0
  • Peer Reviewed
• [Journal Article] The response to interferon is influenced by hepatitis B virus genotype in vitro and in vivo. (2013)
  • Author(s): Zhang Y, Wu Y, Ye S, Wang T, Zhao R, Chen F, Abe K, Jin X.
  • Journal Title: Virus Research Volume: 171 Issue: 1 Pages: 65-70
  • DOI: 10.1016/j.virusres.2012.10.027
  • Peer Reviewed
• [Journal Article] Rubella epidemic in Vietnam: characteristic of rubella virus genes from pregnant women and their fetuses/newborns with congenital rubella syndrome. (2013)
  • Author(s): Van Hung Pham, Thong Van Nguyen, Truc Thanh Thi Nguyen, Linh Duy Dang, Ngoc Hieu Hoang, Truong Van Nguyen, Kenji Abe.
  • Journal Title: Journal of Clinical Virology Volume: 57 Issue: 2 Pages: 152-156
  • DOI: 10.1016/j.jcv.2013.02.008
  • Peer Reviewed
• [Presentation] Rubella outbreak among children in Vietnam: Molecular characterization of virus and its pathogenesis in human fetuses. (2015)
  • Author(s): Kenji Abe
  • Organizer: 2nd Conference on International Asian Pediatric Infectious Diseases.
  • Place of Presentation: 東京大学
  • Year and Date: 2015-06-20
  • Int'l Joint Research / Invited