Screening of novel vivax malaria transmission-blicking vaccine candidate molecule
| Project/Area Number |
25305009
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 海外学術 |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | Ehime University |
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Principal Investigator |
TORII MOTOMI 愛媛大学, プロテオサイエンスセンター, 教授 (20164072)
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| Co-Investigator(Kenkyū-buntansha) |
Tachibana Mayumi 愛媛大学, プロテオサイエンスセンター, 助教 (00301325)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | 寄生虫学 / マラリア / ワクチン / 三日熱マラリア / 伝播阻止ワクチン / 感染症 / 熱帯病 / 伝播阻止 / 三日熱マラリア原虫 / 伝搬阻止 / 寄生虫 |
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| Outline of Final Research Achievements |
Malaria transmission-blocking vaccines (TBV) aim to interfere with the development of the malaria parasite in the mosquito vector, and thus prevent spread of transmission in a community. We aimed to discover novel Plasmodium vivax TBV candidates by using rodent malaria model and finally selected four target molecules; PvG#3, PvG#6, PvG#13 and PvG#16. We produced the recombinant proteins of these target molecules using wheat germ cell-free system. Rabbit antisera against these recombinant proteins were generated. In IFA, anti-PvG#3, -PvG#6, -PvG#13 and -PvG#16 antisera were reacted on the surface of P. vivax gametocytes. Membrane feeding transmission assays using P. vivax isolates obtained in Thailand demonstrated that anti-PvG#3, anti-PvG#6 and anti-PvG#16 antisera significantly reduced the number of oocysts developing in the mosquito midgut. In conclusion, our results indicate that PvG#3, PvG#6 and PvG#16 are potential transmission-blocking vaccine candidates of P. vivax.
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Report
(4 results)
Research Products
(4 results)
