| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
The geographical distribution, prevalence, and intermediate hosts of the liver fluke belonging to the genus Amphimerus were investigated in Ecuador. Endemic areas were found in 3 provinces in the Pacific Coast. More than 20 species of the freshwater fish were examined for metacercariae. Small fishes called Guabina, which are highly prevalent in the small streams flowing along the villages, were found to be the most important sources of infection in humans. This fish species recorded a high number of metacercariae per fish. We analyzed the sequence of ribosomal ITS2 DNA from adult Amphimerus flukes discharged by patients after praziquantel treatment, and utilized it as a marker to identify the metacercariae of this species in fish. A literature review was also conducted on lung flukes to summarize the status of the disease, paragonimiasis caused by Paragonimus mexicanus.
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