Utilization as resources of fungi and actinobacterium collected in Kenya,Chiba and other areas

• Project/Area Number: 25305012
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Bacteriology (including mycology)
• Research Institution: Chiba University
• Principal Investigator: Gonoi Tohru 千葉大学, 真菌医学研究センター, 教授 (30134365)
• Co-Investigator(Kenkyū-buntansha): 松澤 哲宏 長崎県立大学, 看護栄養学部, 講師 (40598702) ; 矢口 貴志 千葉大学, 真菌医学研究センター, 准教授 (60361440)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 真菌 / 放線菌 / 感染症 / カビ毒 / 食糧汚染 / ヒト病原性 / ケニア / ２次代謝産物 / 遺伝子発現 / 転写因子 / 真菌感染症 / アスペルギルス / 糸状菌 / 病原放線菌 / 国際研究者交流 ケニア / 国際研究者交流 中国 / 国際研究者交流 インドネシア / 遺伝子資源 / 微生物資源 / アフラトキシン / フザリウム / フモニシン / クリプトコッカス / カンジダ

Outline of Final Research Achievements

We visited Kenya in Africa and conducted eppidemiological studies on mycotoxin producing and human and plant pathogenic fungi in collaboration with Kenyan researchers. We found Kenyan cereals were highly contaminated with mycotoxins, and call attention of the natives using news papers and internet HP.

Research Products

• [Int'l Joint Research] KEMRI(Kenya)
• [Int'l Joint Research] 貴陽医学院(China)
• [Int'l Joint Research] University of Indonesia(Indonesia)
• [Int'l Joint Research] Kenya Medical Research INstitute(ケニア)
• [Int'l Joint Research] 貴陽医学院(中国)
• [Int'l Joint Research] カンピーナス大学(ブラジル)
• [Journal Article] Transcription factor Afmac1 controls copper import machinery in Aspergillus fumigatus has been accepted for publication. (2017)
  • Author(s): Kusuya Y, Hagiwara D, Sakai K, Yaguchi T, Gonoi T, Takahashi H.
  • Journal Title: Current Genetics Volume: 67 Issue: 4 Pages: 1-13
  • DOI: 10.1007/s00294-017-0681-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A novel Zn2-Cys6 transcription factor AtrR plays a key role in an azole resistance mechanism of Aspergillus fumigatus by co-regulating cyp51A and cdr1B expressions. (2017)
  • Author(s): Hagiwara D, Miura D, Shimizu K, Paul S, Ohba A, Gonoi T, Watanabe A, Kamei K, Shintani T, Moye-Rowley WS, Kawamoto S, Gomi K.
  • Journal Title: PLOS Pathogens Volume: - Issue: 1 Pages: e1006096-e1006096
  • DOI: 10.1371/journal.ppat.1006096
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Aflatoxin M1 Contamination of Milk and Its Products in Bomet County, Kenya. (2016)
  • Author(s): Gladys Langat, Matsusawa Tetsuhiro, Tohru Gonoi, Vivienne Matiru, Christine Bii
  • Journal Title: Advances Microbiol Volume: 6 Pages: 528-536
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Multi-azole resistant Aspergillus fumigatus harboring Cyp51A TR46/Y121F/T289A isolated in Japan (2016)
  • Author(s): Hagiwara, D; Takahashi H, Fujimoto, M; Sugaharac, M; Misawad Y; Gonoi, T, Itoyamae, S; Watanabea, A; Kamei, K
  • Journal Title: Journal of Infection and Chemotherapy Volume: in press Issue: 8 Pages: 577-579
  • DOI: 10.1016/j.jiac.2016.01.015
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparative transcriptome analysis revealing dormant conidia and germination associated genes in Aspergillus species: an essential role for AtfA in conidial dormancy (2016)
  • Author(s): Hagiwara D, Takahashi H, Kusuya Y, Kawamoto S, Kamei K, Gonoi T
  • Journal Title: BMC Genomics Volume: 17 Issue: 1 Pages: 358-358
  • DOI: 10.1186/s12864-016-2689-z
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Nocardia shinanonensis sp. nov., isolated from a patient with endophthalmitis. (2016)
  • Author(s): Matsumoto T, Negishi T, Hamada M, Komaki H, Gonoi T, Yaguchi T.
  • Journal Title: Int J Syst Evol Microbiol Volume: 66 Issue: 9 Pages: 3324-3328
  • DOI: 10.1099/ijsem.0.001197
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A Japanese Pediatric Case of Primary Cutaneous Nocardiosis (2016)
  • Author(s): Shono, M; Suga K; Gonoi, T; Goji A; Mastuura, S; Inoue, M; Kawahito, M; Yoshii, S; Sedo, H; Mori, K
  • Journal Title: JMM Case Reports Volume: in press
  • Peer Reviewed
• [Journal Article] Identification and Functional Analysis of the Nocardithiocin Gene Cluster in Nocardia pseudobrasiliensis (2015)
  • Author(s): Sakai, K; Komaki, H; Gonoi, T
  • Journal Title: PLOS ONE Volume: 10 Issue: 11 Pages: e0143264-e0143264
  • DOI: 10.1371/journal.pone.0143264
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Tyrokeradines G and H, new bromotyrosine alkaloids from an Okinawan Verongid sponge (2015)
  • Author(s): Kubota, T.; Watase, S.; Sakai, K.; Fromont, J.; Gonoi, T.; Kobayashi, J.
  • Journal Title: Bioorg. Med. Chem. Lett. Volume: 25 Issue: 22 Pages: 5221-5223
  • DOI: 10.1016/j.bmcl.2015.09.061
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Identification of genes involved in the phosphate metabolism in Cryptococcus neoformans (2015)
  • Author(s): Toh-e, A; Ohkusu, M; Li, HM; Shimizu, K; Takahashi-Nakaguchi, A; Gonoi, T; Kawamoto, S; Kanesaki, Y; Yoshikawa, H; Nishizawa, M
  • Journal Title: FUNGAL GENETICS AND BIOLOGY Volume: 80 Pages: 19-30
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] MOLECULAR IDENTIFICATION AND ANTIMICROBIAL RESISTANCE PATTERN OF SEVEN CLINICAL ISOLATES OF Nocardia spp. IN BRAZIL (2015)
  • Author(s): Condas, LAZ; Ribeiro, MG; Muro, MD; de Vargas, APC; Matsuzawa, T; Yazawa, K; Siqueira, AK; Salerno, T; Lara, GHB; Risseti, RM; Ferreira, KS; Gonoi, T
  • Journal Title: REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO Volume: 57 Issue: 3 Pages: 251-256
  • DOI: 10.1590/s0036-46652015000300012
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genome sequence comparison of Aspergillus fumigatus strains isolated from patients with pulmonary aspergilloma and chronic necrotizing pulmonary aspergillosis (2015)
  • Author(s): Takahashi-Nakaguchi A, Yasunori Muraosa Y, Hagiwara D, Sakai K,Toyotome T, Watanabe A, Kawamoto S, Kamei K, Gonoi T, Takahashi H
  • Journal Title: Medical Mycology Volume: 53 Issue: 4 Pages: 353-360
  • DOI: 10.1093/mmy/myv003
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Gordonia iterans sp. nov., isolated from a patient with pneumonia. (2014)
  • Author(s): Kang Y, Ming H, Gonoi T, Chen Y, Cao Y,Wang Y, Cheng J, Koga T, Mikami Y, Li W-J
  • Journal Title: Int J Syst Evol Microbiol Volume: 64 Issue: Pt_10 Pages: 3520-3525
  • DOI: 10.1099/ijs.0.063438-0
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of fungal pathogens by visivle microarray system in combination with isothermal gene amplification (2014)
  • Author(s): Sakai K, Trabasso P, Moretti ML, Mikami Y, Kamei K, Gonoi T.
  • Journal Title: Mycopathologia Volume: 印刷中 Issue: 1-2 Pages: 11-26
  • DOI: 10.1007/s11046-014-9756-2
  • Peer Reviewed / Open Access
• [Journal Article] Genome based analysis of type-I polyketide synthase and nonribosomal peptide synthetase gene clusters in seven strains of five representative Nocardia species (2014)
  • Author(s): Hisayuki Komaki, Natsuko Ichikawa, Akira Hosoyama, Azusa Takahashi-Nakaguchi, Tetsuro Matsuzawa,Ken-ichiro Suzuki,Nobuyuki Fujita, Tohru Gonoi
  • Journal Title: BMC genomics Volume: 15 Issue: 1 Pages: 1-11
  • DOI: 10.1186/1471-2164-15-323
  • Peer Reviewed / Open Access
• [Journal Article] Total aflatoxin, fumonisin and deoxynivalenol contamination of busaa in Bomet county, Kenya. (2014)
  • Author(s): Kirui MC, Alakonya AE, Talam KK, Gonoi T, Bii CC.
  • Journal Title: African Journal of Biotechnology Volume: 13 Issue: 26 Pages: 2675-2678
  • DOI: 10.5897/ajb2014.13754
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Lectin-microarray technique for glycomic profiling of fungal cell surfaces. (2014)
  • Author(s): Shibazaki A, Gonoi T.
  • Journal Title: Methods Mol Biol Volume: 1200 Pages: 287-294
  • DOI: 10.1007/978-1-4939-1292-6_24
  • ISBN: 9781493912919, 9781493912926
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Whole-genome comparison of Aspergillus fumigatus strains serially isolated from patients infected with aspergillosis. (2014)
  • Author(s): Hagiwara D, Takahashi H, Watanabe A, Takahashi-Nakaguchi A, Kawamoto S, Kamei K, Gonoi T
  • Journal Title: J Clin Microbiol Volume: 52 Issue: 12 Pages: 4202-4209
  • DOI: 10.1128/jcm.01105-14
  • Peer Reviewed / Open Access
• [Journal Article] The role of AtfA and HOG MAPK pathway in stress tolerance in conidia of Aspergillus fumigatus. (2014)
  • Author(s): Hagiwara D, Suzuki S, Kamei K, Gonoi T, Kawamoto S.
  • Journal Title: Fungal Genetics and Biology Volume: 73 Pages: 138-49
  • DOI: 10.1016/j.fgb.2014.10.011
  • Peer Reviewed / Open Access
• [Journal Article] Modified Slide Culture Method for Faster and Easier Identification of ermatophytes (2014)
  • Author(s): YEVA ROSANA, TETSUHIRO MATSUZAWA , TOHRU GONOI
  • Journal Title: Microbiology Indonesia Volume: 8
  • Peer Reviewed / Open Access
• [Journal Article] Aspergillus huiyaniae sp. nov., a teleomorphic species in sect. Fumigati isolated from desert soil in China. (2014)
  • Author(s): Matsuzawa T, Horie Y, Abliz P, Gonoi T, Yaguchi T
  • Journal Title: Mycoscience Volume: 55 Issue: 3 Pages: 213-220
  • DOI: 10.1016/j.myc.2013.08.007
  • Peer Reviewed
• [Journal Article] Document Molecular identification and antimicrobial susceptibility of Nocardia spp. isolated from bovine mastitis in Brazil (2013)
  • Author(s): Condas LAZ, Ribeiro MG, Yazawa K, de Vargas APC, Salerno T, Giuffrida R, Langoni H, Melville PA, Biesdorf S, Matsuzawa T, Gonoi T, Kastelic JP, Barkema HW
  • Journal Title: Veterinary Microbiology Volume: 167 Issue: 3-4 Pages: 708-712
  • DOI: 10.1016/j.vetmic.2013.08.019
  • Peer Reviewed
• [Journal Article] Ultra-deep sequencing analysis of the hepatitis A virus 5'-untranslated region among cases of the same outbreak from a single source (2013)
  • Author(s): Wu S., et al.
  • Journal Title: Int J Med Sci. Volume: 11 Issue: 1 Pages: 60-64
  • DOI: 10.7150/ijms.7728
  • Peer Reviewed / Open Access
• [Journal Article] NikA/TcsC histidine kinase is involved in conidiation, hyphal morphology, and responses to osmotic stress and antifungal chemicals in Aspergillus fumigatus (2013)
  • Author(s): Hagiwara D, Takahashi-Nakaguchi A, Toyotome T, Yoshimi A, Abe K, Kamei K, Gonoi T, Kawamoto S.
  • Journal Title: Plos ONE Volume: 8 Issue: 12 Pages: 1-15
  • DOI: 10.1371/journal.pone.0080881
  • Peer Reviewed
• [Journal Article] Visual analysis of Dna Microarray data for accurate molecular identification of non-Albicans Candida isolates from patients with Candidemia episodes (2013)
  • Author(s): De Luca Ferrari M, Ribeiro Resende M, Sakai K, Muraosa Y, Lyra L, Gonoi T, Mikami Y, Tominaga K, Kamei K, Zaninelli Schreiber A, Trabasso P, Luiza Moretti M
  • Journal Title: Journal of Clinical Microbiology Volume: 51 Issue: 11 Pages: 3826-3829
  • DOI: 10.1128/jcm.01050-13
  • Peer Reviewed
• [Presentation] Genomic and transcriptomic analyses of human pathogenic fungi, Aspergillus fumigatus (2016)
  • Author(s): Gonoi T
  • Organizer: The 6 th ASIA PACIFIC SOCIETY for MEDICAL MYCOLOGY CONGRESS
  • Place of Presentation: Bali, Indonesia
  • Year and Date: 2016-10-20
  • Int'l Joint Research / Invited
• [Presentation] on in Nocardia pseudobrasiliensis by co-culture with animal cells (2015)
  • Author(s): Sakai K, Gonoi T
  • Organizer: International Society of Human and Animal Mycology
  • Place of Presentation: Melbourne (Australia)
  • Year and Date: 2015-05-04
  • Int'l Joint Research
• [Presentation] Identification of fungal pathogens by visible microarray system in combination with isothermal gene amplification (2015)
  • Author(s): Gonoi, T; Sakai, K
  • Organizer: International Society of Human and Animal Mycology
  • Place of Presentation: Melbourne (Australia)
  • Year and Date: 2015-05-04
  • Int'l Joint Research / Invited
• [Presentation] Identification of mycovirus genes associated with reduced pathogenicity of Aspergillus fumigatus (2015)
  • Author(s): Shishido, E; Takahashi-Nakaguchi, A; Ando, A; Moriyama, H; Gonoi, T.
  • Organizer: International Society of Human and Animal Mycology
  • Place of Presentation: Melbourne (Australia)
  • Year and Date: 2015-05-04
  • Int'l Joint Research
• [Presentation] MLST of Fusarium spp. isolated from blood cultures of immunocompromised patients and from the air sampling of hematology units (2015)
  • Author(s): Busso-Lopes A, Moraes, R; Tararan, C; Muraosa, Y; Yuzuru Mikami, Y; Trabasso, P; Reichert-Lima F; Stivanelli, P; Lyra, L; Gonoi, T; Taguchi, H; Schreiber, AZ; Kamei,K; Moretti, ML
  • Organizer: International Society of Human and Animal Mycology
  • Place of Presentation: Melbourne (Australia)
  • Year and Date: 2015-05-04
  • Int'l Joint Research
• [Presentation] A novel transcriptional regulator AtrR is required for cdr1B expression and cyp51A-related azole resistance in Aspergillus fumigatus (2015)
  • Author(s): Hagiwara, D; Shimizu, K; Watanabe, A; Ohba, A; Kamei K; Gonoi, T; Kawamoto, S; Gom, K
  • Organizer: International Society of Human and Animal Mycology
  • Place of Presentation: Melbourne (Australia)
  • Year and Date: 2015-05-04
  • Int'l Joint Research / Invited
• [Presentation] Environment as a Potential Source of Fusarium spp. Invasive Infections in Immunocompromised Patients (2014)
  • Author(s): Moretti ML, Busso-Lopes A, Moraes R, Muraosa Y, Mikami Y, Plinio Trabasso P, Tominaga K, Reichert-Lima F, Lyra L, Gonoi T,Taguchi H, Schreiber A, Katsuhiko Kamei K
  • Organizer: Infectious Diseases Society of America annual meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2014-10-08 – 2014-10-12
• [Presentation] A novel dsRNA mycovirus associated with reduced pathogenicity of Aspergillus fumigatus in a mouse infection model (2014)
  • Author(s): Azusa Takahashi-Nakaguchi, Misa Yahara, Erika Shishido, Hiromitsu Moriyama, Tohru Gonoi
  • Organizer: International Union of Microbiological Society
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2014-07-27 – 2014-08-01
• [Presentation] Screening and functional analysis of novel pathogenic factors in Aspergilluys fumigatus. (2014)
  • Author(s): Kanae Sakai, Tohru Gonoi
  • Organizer: International Union of Microbiological Society
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2014-07-27 – 2014-08-01
• [Presentation] A novel dsRNA mycovirus reduces pathogenicity of Aspergillus fumigatus in a mouse infection model. (2014)
  • Author(s): Azusa Takahashi-Nakaguchi1, Misa Yahara1, Erika Shishido1, Hiromitsu Moriyama2, Tohru Gonoi
  • Organizer: IUMS (International Union of Microbial Society)
  • Place of Presentation: Toronto, Canada
• [Presentation] Screening and functional analysis of novel pathogenic factor in Aspergillus fumigatus. (2014)
  • Author(s): Kanae Sakai, Tohru Gonoi
  • Organizer: IUMS (International Union of Microbial Society)
  • Place of Presentation: Toronto, Canada
• [Presentation] Characterization of Aspergillus species isolated from cereals in aflotoxigenic hot zones in Kenya (2014)
  • Author(s): OMM Mashedi, LW Muita, RK Korir, EG Gatumwa, AK Kulo, T Yaguchi, K Yokoyama, S Kawamoto, T Matsuzawa, CB Bii, Gonoi T
  • Organizer: 6th ADVANCES AGAINST ASPERGILLOSIS
  • Place of Presentation: Madrid, Spain
• [Presentation] Comparison of molecular techniques for identification of Fusarium species from clinical samples (2013)
  • Author(s): Marcela Souz, Tetsuhiro Matsuzawa, Tohru Gonoi, Katsuhiko Kamei, Yasunori Muraosa, Angelica Schreiber, Luzia Lyra, Maria Luiza Moretti, Plinio Trabasso
  • Organizer: Infectious Disease Society of America
  • Place of Presentation: San Francisco, California, USA
• [Book] Lectin-Microarray Technique for Glycomic Profiling of Fungal Cell Surfaces.in Methods in Molecular Biology (2014)
  • Author(s): Shibazaki A, Gonoi T
  • Total Pages: 5
  • Publisher: Springer
• [Remarks] 五ノ井(Gonoi) PI 真菌・放線菌と宿主の分子相互作用研究プロジェクト
  • URL: http://www.pf.chiba-u.ac.jp/research/project/gonoi.html
• [Remarks] 千葉大学真菌医学研究センター
  • URL: http://www.pf.chiba-u.ac.jp/
• [Remarks] 千葉大学真菌医学研究センター・微生物資源分野
  • URL: http://www.pf.chiba-u.ac.jp/research/scientists_list/gonoi.html
• [Remarks] Digital Atlas of Actinomycetes: Nocardia etc.
  • URL: http://www0.nih.go.jp/saj/DigitalAtlas2/atlas.cgi?contrib=A.%20Takahashi-Nakaguchi
• [Funded Workshop] The 6 th ASIA PACIFIC SOCIETY for MEDICAL MYCOLOGY CONGRESS (2016)
  • Place of Presentation: Bali, Indonesia
  • Year and Date: 2016-10-20