| Project/Area Number |
25305015
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 海外学術 |
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| Research Field |
Virology
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| Research Institution | Nagasaki University |
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Principal Investigator |
MORITA Kouichi 長崎大学, 熱帯医学研究所, 教授 (40182240)
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| Co-Investigator(Kenkyū-buntansha) |
HASEBE Futoshi 長崎大学, 国際連携研究戦略本部, 教授 (20253693)
INOUE Shingo 長崎大学, 熱帯医学研究所, 助教 (00346925)
鍋島 武 長崎大学, 熱帯医学研究所, 助教 (30546859)
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| Co-Investigator(Renkei-kenkyūsha) |
YU Fuxun 長崎大学, 熱帯医学研究所, 助教 (30437842)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Keywords | アルボウイルス / プロテオミクス / ゲノミクス / 環境政策 / 環境分析 |
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| Outline of Final Research Achievements |
We collected several thousand clinical (sera) and environmental (mosquitoes) samples in the Philippines, Kenya, Myanmar, Nepal and Japan for virus isolation. Viruses were isolated by inoculating the samples to cultured cells such as mosquito cell line (C6/36) and mammalian cell lines (Vero, LLC-MK2, etc). Culture supernatant of cells showing cytopathic effect were subjected to PCR, nLC-MS assay and NGS sequencing. A number of known viruses were identified namely, dengue virus, chikungunya virus, Rift Valley fever virus and entero viruses while some unidentified viruses e.g. Tanay virus and Tofla virus were recently recognized. In this research program, we established a procedure to facilitate the detection of unknown virus combining proteome and genome information.
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