Comprehensive survey of arboviruses in the tropics

• Project/Area Number: 25305015
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Virology
• Research Institution: Nagasaki University
• Principal Investigator: MORITA Kouichi 長崎大学, 熱帯医学研究所, 教授 (40182240)
• Co-Investigator(Kenkyū-buntansha): HASEBE Futoshi 長崎大学, 国際連携研究戦略本部, 教授 (20253693) ; INOUE Shingo 長崎大学, 熱帯医学研究所, 助教 (00346925) ; 鍋島 武 長崎大学, 熱帯医学研究所, 助教 (30546859)
• Co-Investigator(Renkei-kenkyūsha): YU Fuxun 長崎大学, 熱帯医学研究所, 助教 (30437842)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
• Keywords: アルボウイルス / プロテオミクス / ゲノミクス / 環境政策 / 環境分析

Outline of Final Research Achievements

We collected several thousand clinical (sera) and environmental (mosquitoes) samples in the Philippines, Kenya, Myanmar, Nepal and Japan for virus isolation. Viruses were isolated by inoculating the samples to cultured cells such as mosquito cell line (C6/36) and mammalian cell lines (Vero, LLC-MK2, etc). Culture supernatant of cells showing cytopathic effect were subjected to PCR, nLC-MS assay and NGS sequencing. A number of known viruses were identified namely, dengue virus, chikungunya virus, Rift Valley fever virus and entero viruses while some unidentified viruses e.g. Tanay virus and Tofla virus were recently recognized. In this research program, we established a procedure to facilitate the detection of unknown virus combining proteome and genome information.

Research Products

• [Journal Article] Tofla virus: A newly identified Nairovirus of the Crimean-Congo hemorrhagic fever group isolated from ticks in Japan. (2016)
  • Author(s): Shimada S, Aoki K, Nabeshima T, Fuxun Y, Kurosaki Y, Shiogama K, Onouchi T, Sakaguchi M, Fuchigami T, Ono H, Nishi K, Posadas-Herrera G, Uchida L, Takamatsu Y, Yasuda J, Tsutsumi Y, Fujita H, Morita K, Hayasaka D.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 20213-20213
  • DOI: 10.1038/srep20213
  • NAID: 120006666534
  • Peer Reviewed / Open Access
• [Journal Article] Isolation of dengue serotype 3 virus from the cerebrospinal fluid of an encephalitis patient in Hai Phong, Vietnam in 2013. (2015)
  • Author(s): Phu Ly MH, Takamatsu Y, Nabeshima T, Pham Hoai LL, Pham Thi H, Dang Thi D, Nguyen NL, Nguyen Thi TT, Le Thi QM, Buerano CC, Morita K, Hasebe F.
  • Journal Title: J Clin Virol. Volume: 70 Pages: 93-96
  • DOI: 10.1016/j.jcv.2015.07.295
  • NAID: 120006986955
  • Peer Reviewed / Open Access
• [Journal Article] Molecular epidemiology of Japanese encephalitis in northern Vietnam, 1964-2011: genotype replacement. (2015)
  • Author(s): Do LP, Bui TM, Hasebe F, Morita K, Phan NT.
  • Journal Title: Virol J. Volume: 12 Issue: 1 Pages: 51-51
  • DOI: 10.1186/s12985-015-0278-4
  • NAID: 120006987080
  • Peer Reviewed / Open Access
• [Journal Article] Detection of Chikungunya Virus in Nepal. (2015)
  • Author(s): Pandey BD, Neupane B, Pandey K, Tun MM, Morita K.
  • Journal Title: Am J Trop Med Hyg. Volume: 93(4) Issue: 4 Pages: 697-700
  • DOI: 10.4269/ajtmh.15-0092
  • Peer Reviewed / Open Access
• [Journal Article] Molecular Epidemiology of Dengue Viruses Co-circulating in Upper Myanmar in 2006 (2015)
  • Author(s): Kyaw Zin Thant, Mya Myat Ngwe Tun, Maria del Carmen Parquet, Shingo Inoue, Yee Yee Lwin, Sanda Lin, Kay Thi Aye, Pe Thet Khin, Tin Myint, Khin Htwe, Takeshi Nabeshima and Kouichi Morita.
  • Journal Title: Tropical Medicine and Health Volume: 43 Issue: 1 Pages: 21-27
  • DOI: 10.2149/tmh.2014-27
  • NAID: 130004704897
  • ISSN: 1348-8945, 1349-4147
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A Dengue virus serotype 4-dominated outbreak in central Vietnam, 2013 (2015)
  • Author(s): Yuki Takamatsu, Takeshi Nabeshima, Thuy Thi Thu Nguyen, Dinh Thi Dang, Ly Hoai Linh Pham, Hang Thi Pham, Lien Thi Nam Nguyen, Huong Ly Minh Phu, Thuy Thu Bui, Mai Thi Quynh Le, Kouichi Morita, Futoshi Hasebe.
  • Journal Title: Journal of Clinical Virology Volume: 66 Pages: 24-26
  • DOI: 10.1016/j.jcv.2015.02.016
  • NAID: 120006987102
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Detection of East/Central/South African Genotype of Chikungunya Virus in Myanmar, 2010 (2014)
  • Author(s): Ngwe Tun MM, Thant KZ, Inoue S, Nabeshima T, Aoki K, Kyaw AK, Myint T, Tar T, Maung KTT, Hayasaka D, Morita K.
  • Journal Title: Emerging Infectious Diseases Volume: 20 Issue: 8 Pages: 1378-1381
  • DOI: 10.3201/eid2008.131431
  • NAID: 120006986459
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Tanay virus, a new species of virus isolated from mosquitoes in the Philippines (2014)
  • Author(s): T. Nabeshima, S. Inoue, K. Okamoto, P.H. Guillermo, F. Yu, L. Uchida, A. Ichinose, M. Sakaguchi, T. Sunahara, C.C. Buerano, F.P. Tadena, I.B. Orbita, F.F. Natividad, and K. Morita
  • Journal Title: Journal of General Virology Volume: - Issue: 6 Pages: 1390-1395
  • DOI: 10.1099/vir.0.061887-0
  • Peer Reviewed
• [Journal Article] Tanay virus, a new species of virus isolated from mosquitoes in the Philippines. (2014)
  • Author(s): Takeshi Nabeshima, Shingo Inoue, Kenta Okamoto, Guillermo Posadas-Herrera, Fuxun Yu, Leo Uchida, Akitoyo Ichinose, Miako Sakaguchi, Toshihiko Sunahara, Corazon C. Buerano, Florencio P. Tadena, Ildefonso B. Orbita, Filipinas F.
  • Journal Title: Journal of General Virology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] An approach for differentiating Echovirus 30 and Japanese encephalitis virus infections in acute (2013)
  • Author(s): Yuki Takamatsu, Leo Uchida, Phan Thi Nga, Kenta Okamoto, Takeshi Nabeshima, Dang Thi Thu Thao, Do Thien Hai, Nguyen Thi Tuyet, Hoang Minh Duc, Le Xuan Luat, Futoshi Hasebe and Kouichi Morita.
  • Journal Title: Virology Journal Volume: 10 Pages: 280-280
  • Peer Reviewed
• [Presentation] Tofla virus: a new Nairovirus belonging to the Crimean-Congo hemorrhagic fever group isolated from ticks in Japan. (2015)
  • Author(s): Satoshi Shimada, Kotaro Aoki, Takeshi Nabeshima, Yu Fuxun, Yohei Kurosaki, Kazuya Shiogama, Takanori Onouchi, Miako Sakaguchi, Posadas-Herrera Guilermo, Leo Uchida, Yuki Takamatsu, Jiro Yasuda, Yutaka Tsutsumi, Hiromi Fujita, Kouichi Morita, Daisuke Hayasaka.
  • Organizer: The 63rd Annual Meeting of the Japanese Society for Virology.
  • Place of Presentation: 福岡国際会議場（福岡県・福岡市）
  • Year and Date: 2015-11-22
• [Presentation] Dengue outbreak in Myanmar 2013: A decade persistence of predominant dengue virus serotype 1. (2015)
  • Author(s): Ngwe Tun Mya Myat, Kyaw Aung Kyaw, Nader Makki, Rohitha Muthugala, Takeshi Nabeshima, Shingo Inoue, Daisuke Hayasaka, Myat Thwe Saw Zin, Kouichi Morita.
  • Organizer: The 63rd Annual Meeting of the Japanese Society for Virology.
  • Place of Presentation: 福岡国際会議場（福岡県・福岡市）
  • Year and Date: 2015-11-22
• [Presentation] A dengue virus serotype 4 dominant outbreak in central Vietnam, 2013. (2015)
  • Author(s): Nguyen Thi Thu Thuy, Yuki Takamatsu, Dang thi Dinh, Nguyen Nam Lien, Dang Hoai Linh Ly, Pham Thi Hang, Bui Thu Thuy, Takeshi Nabeshima, Le Quynh Mai, Kouichi Morita, Futoshi Hasebe.
  • Organizer: The 63rd Annual Meeting of the Japanese Society for Virology.
  • Place of Presentation: 福岡国際会議場（福岡県・福岡市）
  • Year and Date: 2015-11-22
• [Presentation] Production, characterization and diagnostic application of monoclonal antibodies against yellow fever virus. (2015)
  • Author(s): Fernand Adungo, Yu Fusun, David Kamau, Inoue Shingo, Hayasaka Daisuke, Guillemo Pasadas-Herrera, Rosemary Sang, Mwau Matilu, Morita Kouich.
  • Organizer: The 14th Awaji International Forum on Infection and Immunity.
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県・淡路市）
  • Year and Date: 2015-09-08
  • Int'l Joint Research
• [Presentation] 国内のマダニから分離された新規ナイロウイルス. (2015)
  • Author(s): 嶋田聡, 青木康太郎, 鍋島武, 余福勲, 黒崎陽平, 塩竈和也, 尾之内高慶, 坂口美亜子, 淵上剛, 小野北斗, 西弘大, Guillermo Posadas-Herrera, 内田玲麻, 高松由基, 安田二朗, 堤寛, 藤田博己, 森田公一, 早坂大輔.
  • Organizer: 第52回日本ウィルス学会九州支部総会.
  • Place of Presentation: 別府ビーコンプラザ 中会議室（大分県・別府市）
  • Year and Date: 2015-09-04
• [Presentation] 国内のマダニから分離された新規ナイロウイルス. (2015)
  • Author(s): 嶋田聡, 青木康太郎, 鍋島武, 余福勲, 黒崎陽平, 塩竈和也, 尾之内高慶, 坂口美亜子, 淵上剛, 小野北斗, 西弘大, Guillermo Posadas-Herrera, 内田玲麻, 高松由基, 安田二朗, 堤寛, 藤田博己, 森田公一, 早坂大輔.
  • Organizer: 第50回日本脳炎ウイルス生態学研究会.
  • Place of Presentation: ホテル京都エミナース（京都府・京都市）
  • Year and Date: 2015-05-15
• [Presentation] ケニアにおけるチクングニアの発生報告. (2015)
  • Author(s): 井上真吾, Caloline Wsonga, James Kimotho, 森田公一, Rosemary Sang, Lilian Musila.
  • Organizer: 第50回日本脳炎ウイルス生態学研究会.
  • Place of Presentation: ホテル京都エミナース（京都府・京都市）
  • Year and Date: 2015-05-15
• [Presentation] 長崎県の自然環境に生息する蚊やダニが媒介するウイルス病 (2014)
  • Author(s): 森田公一
  • Organizer: 第２６回日本環境動物昆虫学会年次大会
  • Place of Presentation: 長崎大学文教キャンパス（長崎市）
  • Year and Date: 2014-11-29 – 2014-11-30
  • Invited
• [Presentation] Development of monoclonal antibodies against SFTS virus nucleocapsid protein and application in sero-diagnosis (2014)
  • Author(s): 余福勲、Adungo Ferdinard、早坂大輔、森田公一
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-11-10 – 2014-11-12
• [Presentation] わが国への侵入が危惧されるフラビウイルス感染症 (2014)
  • Author(s): 森田公一
  • Organizer: 第14回ヒトと動物の共通感染症研究会学術集会
  • Place of Presentation: 国立感染症研究所（東京都新宿区）
  • Year and Date: 2014-11-08
  • Invited
• [Presentation] ケニアインド洋沿岸におけるデング熱の発生報告 (2014)
  • Author(s): 井上真吾，Allan ole Kwallah, Salame Ashur, Mulati Omuyundo, Missiani Ochwoto, Samson Muuo, Lucy Okubi, Matilu Mwau, 森田公一
  • Organizer: 第49回日本脳炎ウイルス生態学研究会
  • Place of Presentation: ホテルニュー田中（山口市）
  • Year and Date: 2014-05-16 – 2014-05-17
• [Presentation] 未知の蚊媒介性ウイルス (2013)
  • Author(s): 鍋島武，井上真吾，岡本健太，H. Guillermo Posadas，Yu Fuxun，F. Natividad
  • Organizer: 第54回日本熱帯医学会大会
  • Place of Presentation: 長崎ブリックホール（長崎市）
• [Presentation] ベトナム国の急性髄膜炎/脳炎患者におけるエコーウイルス30感染例と日本脳炎ウイルス感染例の臨床比較解析。 (2013)
  • Author(s): 高松由基，内田玲麻，Phan Thi Nga，岡本健太，鍋島武，Dang Thi Thu Thao，Do
  • Organizer: 第54回日本熱帯医学会大会
  • Place of Presentation: 長崎ブリックホール（長崎市）