Molecular epidemiology and population dynamics of rotaviruses in Asia based on novel comprehensive whole genomic analysis method

• Project/Area Number: 25305022
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Hygiene and public health
• Research Institution: Sapporo Medical University
• Principal Investigator: Nobumichi Kobayashi 札幌医科大学, 医学部, 教授 (80186759)
• Co-Investigator(Kenkyū-buntansha): 鷲見 紋子 札幌医科大学, 医学部, 准教授 (10363699) ; 漆原 範子 札幌医科大学, 医学部, 講師 (80396308) ; ゴッシュ ソウビック 札幌医科大学, 医学部, 助教 (30597175)
• Research Collaborator: AIDA Satoru ; WANG Yuanhong ; PAUL Shyamal
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: ロタウイルス / ゲノム / 分子疫学 / 遺伝子分節 / 遺伝子交雑 / アジア / 下痢症 / 遺伝子型 / 中国 / バングラデシュ / 系統

Outline of Final Research Achievements

Epidemic dynamics for long periods of human rotaviruses in Asia was analyzed molecular epidemiologically based on whole genome (11 gene segments). In Wuhan, China, G3P[8] was the most prevalent genotype of rotaviruses over past 10 years, which was thereafter replaced by G9P[8]. Change of lineages was detected for most of their gene segments with the passage of time. In Bangladesh, two lineages of the most prevalent G2P[4] genotype in 2010 were replaced by a novel lineage after 3 years. These findings indicated that replacement of gene segments via reassortment and mutation occurred over time among rotaviruses with prevalent genotypes. It was suggested that genetic changes have been occurring constantly even among the same genotype of rotaviruses.

Research Products

• [Int'l Joint Research] 武漢市疾病対策制御センター(中国)
• [Int'l Joint Research] マイメンシン医科大学(バングラデシュ)
• [Int'l Joint Research] 武漢市疾病対策制御センター(中国)
• [Int'l Joint Research] マイメンシン医科大学(バングラデシュ)
• [Int'l Joint Research] ヤンゴン第二医科大学(ミャンマー)
• [Journal Article] Distribution of two distinct Rotavirus B (RVB) strains in the north-central Bangladesh and evidence for reassortment event among human RVB revealed by whole genomic analysis (2017)
  • Author(s): Aung MS, Nahar S, Aida S, Paul SK , Hossain MA, Ahmed S, Haque N, Ghosh S, Malik YS , Urushibara N, Kawaguchiya M, Sumi A, Kobayashi N
  • Journal Title: Infection Genetics and Evolution Volume: 47 Pages: 77-86
  • DOI: 10.1016/j.meegid.2016.11.001
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Multispecies reassortant bovine rotavirus strain carries a novel simian G3-like VP7 genotype (2016)
  • Author(s): Malik YS , Kumar N, Sharma K, Saurabh S, Dhama K, Prasad M, Ghosh S, Bányai K, Kobayashi N, Singh RK.
  • Journal Title: Infection Genetics and Evolution Volume: 41 Pages: 63-72
  • DOI: 10.1016/j.meegid.2016.03.023
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Whole genomic analysis of G2P[4] human Rotaviruses in Mymensingh, north-central Bangladesh (2016)
  • Author(s): Aida S, Nahar S, Paul SK, Hossain MA, Kabir MR, Sarkar SR, Ahmed S, Ghosh S, Urushibara N, Kawaguchiya M, Aung MS, Sumi A, Kobayashi N
  • Journal Title: Heliyon Volume: 2 Issue: 9 Pages: e00168-e00168
  • DOI: 10.1016/j.heliyon.2016.e00168
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Genomic characterization of G3P[6], G4P[6] and G4P[8] human rotaviruses from Wuhan, China: Evidence for interspecies transmission and reassortment events. (2015)
  • Author(s): Zhou X, Wang Y-H, Ghosh S, Tang W-F, Pang B-B, Liu M-Q, Peng J-S, ZhouD-J, Kobayashi N.
  • Journal Title: Ｉｎｆｅｃｔｉｏｎ Ｇｅｎｅｔｉｃｓ ａｎｄ Ｅｖｏｌｕｔｉｏｎ Volume: 33 Pages: 55-71
  • DOI: 10.1016/j.meegid.2015.04.010
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Analysis of codon usage pattern evolution in avian rotaviruses and their preferred host. (2015)
  • Author(s): Kattoor JJ, Malik YS, Sasidharan A, Rajan VM, Dhama K, Ghosh S, Bányai K, Kobayashi N, Singh RK
  • Journal Title: Ｉｎｆｅｃｔｉｏｎ Ｇｅｎｅｔｉｃｓ ａｎｄ Ｅｖｏｌｕｔｉｏｎ Volume: 34 Pages: 17-25
  • DOI: 10.1016/j.meegid.2015.06.018
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Whole genomic analysis of a porcine G6P[13] rotavirus strain. (2015)
  • Author(s): Ghosh S, Navarro R, Malik YS, Willingham AL, Kobayashi N
  • Journal Title: Ｖｅｔｅｒｉｎａｒｙ Ｍｉｃｒｏｂｉｏｌｏｇｙ Volume: 18 Issue: 3-4 Pages: 286-298
  • DOI: 10.1016/j.vetmic.2015.09.017
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Exotic rotaviruses in animals and rotaviruses in exotic animals. (2014)
  • Author(s): Ghosh S, Kobayashi N
  • Journal Title: VirusDisease Volume: 25 Issue: 2 Pages: 158-172
  • DOI: 10.1007/s13337-014-0194-z
  • Peer Reviewed
• [Journal Article] Molecular epidemiology and genetic evolution of the whole genome of G3P[8] human rotavirus in Wuhan, China, from 2000 through 2013 (2014)
  • Author(s): Wang YH, Pang BB, Ghosh S, Zhou X, Shintani T, Urushibara N, Song YW, He MY, Liu MQ, Tang WF, Peng JS, Hu Q, Zhou DJ, Kobayashi N
  • Journal Title: Ｐｌｏｓ ＯＮＥ Volume: 9 Issue: 3 Pages: e88850-e88850
  • DOI: 10.1371/journal.pone.0088850
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Hospital based surveillance and genetic characterization of rotavirusstrains in children ( (2014)
  • Author(s): Mullick S, Mandal P, Nayak MK, Ghosh S, De P, Rajendran K, Bhattacharya MK, Mitra U, Ramamurthy T, Kobayashi N, Chawla-Sarkar M.
  • Journal Title: Ｖａｃｃｉｎｅ Volume: 32 Suppl 1 Pages: A20-A28
  • DOI: 10.1016/j.vaccine.2014.03.018
  • Peer Reviewed / Open Access
• [Journal Article] Complex evolutionary patterns of two rare human G3P[9] rotavirus strains possessing a feline/canine-like H6 genotype on an AU-1-like genotype constellation. (2013)
  • Author(s): Wang YH, Pang BB, Zhou X, Ghosh S, Tang WF, Peng JS, Hu Q, Zhou DJ, Kobayashi N.
  • Journal Title: Infect Genet Evol Volume: 16C Pages: 103-112
  • DOI: 10.1016/j.meegid.2013.01.016
  • Peer Reviewed
• [Journal Article] Whole genomic analyses of asymptomatic human G1P[6], G2P[6] and G3P[6] rotavirus strains reveal intergenogroup reassortment events and genome segments of artiodactyl origin. (2013)
  • Author(s): Ghosh S, Urushibara N, Chawla-Sarkar M, Krishnan T, Kobayashi N.
  • Journal Title: Infect Genet Evol Volume: 16C Pages: 165-173
  • DOI: 10.1016/j.meegid.2012.12.019
  • Peer Reviewed
• [Journal Article] The origin of two rare human P[10] rotavirus strains (2013)
  • Author(s): Ghosh S, Urushibara N, Kawaguchiya M, Shintani T, Kobayashi N
  • Journal Title: Infect Genet Evol Volume: 13 Pages: 292-300
  • DOI: 10.1016/j.meegid.2012.10.021
  • Peer Reviewed
• [Presentation] Whole genomic analysis of human Rotavirus B in Bangladesh: evidence for co-circulation of genetically distinct strains. (2016)
  • Author(s): Kobayashi N, Aung MS, Aida S, Urushibara N, Kawaguchiya M, Sumi A, Paul SK, Nahar S, Hossain MA.
  • Organizer: 50th Joint Working Conference on Viral Diseases US-Japan Cooperative Medical Science Program
  • Place of Presentation: 米国（ベセスダ）
  • Year and Date: 2016-01-13
  • Int'l Joint Research
• [Presentation] Whole genomic analysis of a porcine G5P[13] Rotavirus strain (2016)
  • Author(s): Navarro R, Aung MS, Kobayashi N, Ghosh S
  • Organizer: First Joint AITVM-STVM conference (the Association of Institutions for Tropical Veterinary Medicine (AITVM) and the Society for Tropical Veterinary Medicine (STVM))
  • Place of Presentation: ベルリン(ドイツ）
  • Int'l Joint Research
• [Presentation] Genetic diversity of human Rotavirus B detected in Bangladesh． (2015)
  • Author(s): Aung Meijisoe, Satoru Aida, Samsoon Nahar, Mitsuyo Kawaguchiya, Noriko Urushibara, Shyamal Kumar Paul, Muhammad Akram Hossain, Nobumichi Kobayashi.
  • Organizer: 第63回日本ウイルス学会総会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2015-11-23
• [Presentation] バングラデシュにおけるG2P[4]ヒトロタウイルス株の全遺伝子配列に基づく分子疫学的解析． (2015)
  • Author(s): Satoru Aida, Souvik Ghosh, Noriko Urushibara, Aung Meijisoe, Shyamal Kumar Paul, Nobumichi Kobayashi.
  • Organizer: 第63回日本ウイルス学会総会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2015-11-23
• [Presentation] Analysis on genomic makeup of G3P[6], G4P[6] and G4P[8] rotavirus strains in China. (2015)
  • Author(s): Kobayashi N, Zhou X, Wang YH, Ghosh S.
  • Organizer: 17th International Conference on Emerging Infectious Diseases
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2015-01-26 – 2015-01-27
• [Presentation] Predominance of G9P[8] rotavirus among patients with gastroenteritis in Wuhan, China, during 2011-2014. (2014)
  • Author(s): Wang YH, Zhou X, Pang BB, Tang WF, Peng JS, Hu Q, Zhou DJ, Kobayashi N.
  • Organizer: 7th Asian Congress of Pediatric Infectious Diseases
  • Place of Presentation: Beijing, China
  • Year and Date: 2014-10-13
• [Presentation] Whole genomic analysis of human rotavirus strains causing asymptomatic infections in humans. (2014)
  • Author(s): Kobayashi N, Ghosh S, Sumi A, Taniguchi K, Wang YH.
  • Organizer: 10th China-Japan International Conference of Virology.
  • Place of Presentation: Changchun, China
  • Year and Date: 2014-08-24
• [Presentation] Whole genomic analysis of G3P[6], G4P[6] and G4P[8] rotavirus strains in Wuhan, China. (2014)
  • Author(s): Kobayashi N, Zhou X, Wang YH, Ghosh S, Liu MQ, Pang BB, Tang WF, Peng JS, Hu Q, Zhou DJ.
  • Organizer: International Congress of Virology (IUMS, 2014)
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2014-07-30
• [Presentation] 全ゲノム解析からみた世界のヒトロタウイルスの分子進化と感染伝播の様態 (2014)
  • Author(s): 小林宣道
  • Organizer: 第55回日本臨床ウイルス学会
  • Place of Presentation: 札幌
  • Year and Date: 2014-06-15
  • Invited
• [Presentation] 全遺伝子配列に基づく中国の主流行型G3P[8]ヒトロタウイルスの12年間にわたる分子進化様態の解析 (2013)
  • Author(s): 小林宣道、Ghosh S、新谷つづみ、Wang Y-H、Zhou X、Pang BB
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] Whole genomic analysis of common and unusual equine group A rotavirus strains detected in Japan (2013)
  • Author(s): Ghosh S、谷口孝喜、合田悟、小林宣道
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸