| Project/Area Number |
25330338
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Life / Health / Medical informatics
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| Research Institution | Oita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IWAKI Takafumi 大分大学, 医学部医学科, 助教 (60416419)
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| Co-Investigator(Renkei-kenkyūsha) |
UEDA Kazuyoshi 横浜国立大学, 工学系研究院, 教授 (40223458)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2014: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 生命分子計算 / DNA局所構造変化 / G-quadruplex / DNA / RNA / 円偏光二色性 / ストップトフロー / 分子動力学 / 分子動力学（MD） / 円偏光二色性（CD） / ストップトフロー（緩和時間） / ニコチンアミドモノヌクレオチド（NMN） / Idarubicin / irinotecan / ストップトフロー測定 / 対イオン / DNA凝集 / DNA間相互作用 / 熱安定性 |
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| Outline of Final Research Achievements |
Oligonucletides with a G-rich sequence can form G-quadruplex. G-quadruplex exist in living cells and has drawn attention as a target for gene expression control, cell division, and design of drugs foe cancer. The binding of ligand TMPyP4 with G-quadruplex has been extensively studied. TMPyP4 is well known for the telomerase inhibiter, but interaction of the TMPyP4 and G-quadruplex were studied by ESI-MS, NMR, FRET, and circular dichroism. Irinotecan is a drug used for the treatment of cancer, and prevents DNA from unwinding by inhibition of topoisomerase. We make a hyposys that irinotecan is interact with G-quadruplex, because irinotecan resembles TMPyP4 in action mechanism. Stopped-flow circular dichroism is used to characterize the assembly of complexes consisting of G-quadruplex bound to the ligands. In this study, the rates of each process were determined over the temperature range of 10-50°C and activation energies were determined from the slope of Arrhenius plots.
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