| Project/Area Number |
25330344
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Life / Health / Medical informatics
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| Research Institution | Hiroshima City University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | アミノ酸 / アミノアシルtRNA合成酵素 / バイオセンシング / 酵素 / 病態計測 / 小型装置 / バイオセンサー / マイクロチップ |
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| Outline of Final Research Achievements |
The analysis of free amino acids in plasma is useful for estimating disease status in clinical diagnoses. Large instruments, such as those used for high-performance liquid chromatography, are generally used to analyze amino acids in biological fluids; however, the conventional analytical tools are burdensome in terms of cost, time, and space requirements. Therefore, the development of simple and rapid analytical tools for measuring amino acid concentrations is desirable for the clinical diagnostics.
In this study, the specific interaction between aminoacyl-tRNA synthetase (aaRS) and its corresponding amino acid was used to measure amino acid concentrations. Pyrophosphate released by the amino acid-aaRS binding reaction was detected by the Trinder’s reagent spectrophotometric method.
For the specific detection of glycine, glycyl-tRNA synthetase was used as the recognition element and the method provided selective quantitation of 5–50 µM glycine.
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