Development of a method for identifying functional site in disordered regions of proteins
| Project/Area Number |
25330352
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Life / Health / Medical informatics
|
|---|
| Research Institution | Meiji Pharmaceutical University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
HIROSE Shuichi 長瀬産業株式会社 (60549898)
|
|---|
| Project Period (FY) |
2013-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | 蛋白質 / 機械学習 / 生体生命情報学 / 分子認識 / 天然変性 / ディスオーダー領域 / 機能部位 / PSSM / Masking / Filtering / Smoothing |
|---|
| Outline of Final Research Achievements |
We developed a method for identifying functional site in disordered regions of proteins by using POODLE-S, POODLE-L, PSIPRED, ESPRESSO and Pfam database. POODLE-S and PODDLE-L are methods for predicting short and long disordered regions of proteins, respectively. PSIPRED is a method for predicting protein secondary structures developed by David Jones, et al. ESPRESSO is a system for estimating protein expression and solubility in protein expression systems. Pfam database, is available at EMBL-EBI site, is a large collection of protein families, each represented by multiple sequence alignments and identified their function sites. The prediction accuracy of this method is 78% that is better than desired value (70%), but the True Positive Rate (TPR) of this method is 43% that is less than TPR of other method (57%).
The web system to implement the method has developed in our server. But the system is not available, since the prediction efficiency is not sufficient to use.
|
Report
(5 results)
Research Products
(4 results)
