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Mechanism of accurate chromosome segregation by mitosis-specific phosphorylation of XRCC4

Research Project

Project/Area Number 25340031
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Risk sciences of radiation and chemicals
Research InstitutionOsaka University

Principal Investigator

Terasawa Masahiro  大阪大学, たんぱく質研究所, 特任助教 (20389688)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsDSB修復 / M期 / ゲノム不安定化 / XRCC4 / 非相同末端結合
Outline of Final Research Achievements

Double-strand break (DSB) is the most severe types of DNA damage. To maintain genomic stability, cells possess several checkpoints and DNA repair mechanisms that respond to DNA damage during the G1, S, and G2 phases. A defect in these processes results in apoptosis, aneuploidy, or translocation. However, mitotic cells are known to suppress DSB repair, although the biological significance of the suppression is unknown. This study showed that DSB repair, which prevents genomic instability in other cell cycle phases, is rather toxic to mitotic cells. During mitosis, phosphorylation of XRCC4, a factor of DSB repair, suppresses DSB repair and plays important roles to prevent aberrant chromosome segregation.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (12 results)

All 2016 2015 2014 Other

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) (of which Invited: 2 results) Remarks (2 results)

  • [Journal Article] The MRX Complex Ensures NHEJ Fidelity through Multiple Pathways Including Xrs2-FHA-Dependent Tel1 Activation.2016

    • Author(s)
      Iwasaki D, Hayashihara K, Shima H, Higashide M, Terasawa M, Gasser SM, Shinohara M.
    • Journal Title

      PLoS Genet.

      Volume: 12 Issue: 3 Pages: e1005942-e1005942

    • DOI

      10.1371/journal.pgen.1005942

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Canonical Non-homologous End Joining in Mitosis Induces Genome Instability and Is Suppressed by M-phase Specific Phosphorylation of XRCC4 via CDKs.2014

    • Author(s)
      Terasawa, M., Shinohara A., and M. Shinohara.
    • Journal Title

      PLoS Genetics

      Volume: 10 Issue: 8 Pages: e1004563-e1004563

    • DOI

      10.1371/journal.pgen.1004563

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Double-strand break repair-adox: restoration of suppressed double-strand break repair during mitosis induces genomic instability.2014

    • Author(s)
      Terasawa, M., Shinohara. A., and *M. Shinohara.
    • Journal Title

      Cancer Science

      Volume: 105 Issue: 12 Pages: 1519-1525

    • DOI

      10.1111/cas.12551

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 非相同末端結合因子XRCC4のM期特異的リン酸化はDNA損傷修復抑制を介してゲノム安定性保持に寄与する2015

    • Author(s)
      寺澤匡博、篠原美紀
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸商工会議所、国際展示場
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] 非相同末端結合因子XRCC4のM期特異的リン酸化はDNA損傷修復抑制を介してゲノム安定性保持に寄与する2015

    • Author(s)
      寺澤匡博、篠原美紀
    • Organizer
      第23回DNA複製・組換え修復ワークショップ研究会
    • Place of Presentation
      焼津グランドホテル
    • Year and Date
      2015-10-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] Mitosis-specific phosphorylation of XRCC4 maintains genome stability by suppression of DNA damage repair2015

    • Author(s)
      M. Terasawa, M. Shinohara
    • Organizer
      第74回日本癌学会学術総会 国際シンポジウム「がん染色体動態の分子機構」
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 非相同末端結合因子XRCC4のM期特異的リン酸化はDNA損傷修復抑制を介してゲノム安定性保持に寄与する2014

    • Author(s)
      寺澤匡博、篠原美紀
    • Organizer
      日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 非相同末端結合因子XRCC4のM期特異的リン酸化の染色体分配における役割2014

    • Author(s)
      寺澤匡博、篠原美紀
    • Organizer
      日本放射線影響学会
    • Place of Presentation
      鹿児島
    • Year and Date
      2014-10-01 – 2014-10-03
    • Related Report
      2014 Research-status Report
  • [Presentation] M期における染色体分配とDNA二重鎖切断修復経路制御の連携機構解明

    • Author(s)
      寺澤匡博、篠原美紀
    • Organizer
      第22回DNA複製・組換え・修復ワークショップ
    • Place of Presentation
      仙台
    • Related Report
      2013 Research-status Report
  • [Presentation] A coordination mechanism between chromosome segregation and regulations of DSB repair pathways during mitosis.

    • Author(s)
      Masahiro Terasawa, Akira Shinohara and Miki Shinohara
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Remarks] 守護神が実は破壊者にも!?DNA修復機構はいつでもどこでも守らず、時には壊す側に

    • URL

      http://resou.osaka-u.ac.jp/ja/research/2014/20140829_1

    • Related Report
      2014 Research-status Report
  • [Remarks] DNA修復機構はいつでも守る側ではなく、破壊する側に廻ることも

    • URL

      http://www.protein.osaka-u.ac.jp/jpn/achievement/papers/press-release-dna.php

    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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