Elucidation of radiation biological effect with quantitative and qualitative analyses of base damage cluster
| Project/Area Number |
25340034
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Saga University |
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Principal Investigator |
Terato Hiroaki 佐賀大学, 総合分析実験センター, 准教授 (00243543)
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| Co-Investigator(Kenkyū-buntansha) |
KONDO Toshihiro 佐賀大学, 総合分析実験センター, 教務員 (20186852)
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| Co-Investigator(Renkei-kenkyūsha) |
TOKUYAMA Yuka 佐賀大学, 総合分析実験センター, 教務員 (30398135)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 電離放射線 / DNA損傷 / DNA修復 / 重粒子放射線 / 放射線生物影響 / 線エネルギー付与（LET） / 生物学的効果比（RBE） / 放射線生物効果 / 塩基除去修復 / 重粒子線 / クラスターDNA損傷 / 酸化塩基損傷 / DNAグリコシラーゼ |
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| Outline of Final Research Achievements |
Clustered DNA damage is a specific type of DNA damage with ionizing radiation. In this study, we analyzed the yield of clustered base damage in cultured cells irradiated with various heavy ion beams, and investigated the repair process in post-irradiation cultured cells. Chinese hamster ovary (CHO) cells were irradiated by carbon, silicon, argon and iron ion beams with LETs of 13, 55, 90 and 200 keV/μm, respectively. The cell electrophoresis indicated that clustered base damage yields decreased as the LET increased. Then, clustered DNA damage repair was investigated using DNA repair mutant cells. DNA double-strand breaks accumulated in the mutant cells lacking Xrcc1 after irradiation, and the cell viability decreased. On the other hand, mouse embryonic fibroblast (Mef) cells lacking both Nth1 and Ogg1 became more resistant than the wild type. Thus, clustered base damage seems to be involved in the expression of heavy ion beam biological effects via the repair process.
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Report
(4 results)
Research Products
(23 results)
