| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In order to detect genotoxic compounds in a rapid manner, new fluorescence probes were synthesized. One probe has a property with heat resistant. The Acyclovir moiety is one of the synthetic candidate because the alkoxymethyl moiety at N9 position of guanine is thought to be torrent for the depurinaion condition at elevated temperature. BODIPY-FL was able to be attached to OH group of Acyclovir that had been protected with DMTr at N2 positon. Obtained probe was found to enhance its fluorescence activity when alkylating reagents was treated. The second synthesized probe contained 8-pheny-BODIPY, of which F atom was substituted to OMe group. This probe was attached to deoxyguanosine as a general manner. Treatment of the Fenton reagent to this BODIPY derivative decrease its fluorescence activity as expected. Therefore, a probe to detect 8-oxo-dG formation was pursued. In other probes, oligonucleotide containing BODIPY modified dC is now undertaken to detect DNA strand breaks
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