| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Base modification and AP site in DNA generated by alkylation damage cause cell death and mutagenesis, but the lesions are usually repaired by base excision repair (BER) pathway. Here, we studied initial steps in the repair of DNA alkylation damage by nucleotide excision repair (NER) using fission yeast. NER factors Rhp7p and Rhp41p are involved in repair of AP site. The rhp7 and rph16 genes are induced by different types of DNA damage. Therefore, NER activity not only target UV lesion, but can also remove AP site and subtle base modifications synergistically with BER.
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