Establishment of neonatal animal model for early detection of environmental pollutant induced-learning disability
Project/Area Number |
25340066
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Environmental impact assessment
|
Research Institution | National Institute for Environmental Studies |
Principal Investigator |
Win-Shwe Tin-Tin 国立研究開発法人国立環境研究所, 環境健康研究センター, 主任研究員 (00391128)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 神経毒性 / 学習行動 / 新生児モデル / 環境汚物質 / マウス / 学習・記憶 / 行動 / 新生児 / 脳 / 早期検診 / 環境汚染物質 / 嗅球 / バイマーカー |
Outline of Final Research Achievements |
We have established a novel olfactory-based spatial learning test and examined the effects of exposure to nano-sized diesel exhaust-origin secondary organic aerosol (SOA) on the learning performance in preweaning mice. Pregnant BALB/c mice were exposed to clean air, diesel exhaust (DE), or DE-origin SOA (DE-SOA) from gestational day 14 to postnatal day (PND) 10 in exposure chambers. On PND 11, the mice were examined by the olfactory-based spatial learning test. The mice exposed to DE or DE-SOA took a longer time to reach the target as compared to the control mice. N-methyl-D-aspartate (NMDA) receptor subunits NR1 and NR2B, and immunological markers such as TNF-α, COX2, and Iba1 mRNAs were significantly increased in the hippocampi of the DE-SOA-exposed preweaning mice as compared to the control mice. Our results indicate that DE-SOA exposure during developmental period may affect the olfactory-based spatial learning behavior in preweaning mice.
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Report
(4 results)
Research Products
(6 results)