| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
We have established a novel olfactory-based spatial learning test and examined the effects of exposure to nano-sized diesel exhaust-origin secondary organic aerosol (SOA) on the learning performance in preweaning mice. Pregnant BALB/c mice were exposed to clean air, diesel exhaust (DE), or DE-origin SOA (DE-SOA) from gestational day 14 to postnatal day (PND) 10 in exposure chambers. On PND 11, the mice were examined by the olfactory-based spatial learning test. The mice exposed to DE or DE-SOA took a longer time to reach the target as compared to the control mice. N-methyl-D-aspartate (NMDA) receptor subunits NR1 and NR2B, and immunological markers such as TNF-α, COX2, and Iba1 mRNAs were significantly increased in the hippocampi of the DE-SOA-exposed preweaning mice as compared to the control mice. Our results indicate that DE-SOA exposure during developmental period may affect the olfactory-based spatial learning behavior in preweaning mice.
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