| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The predominant bacterium obtained from the unialgal culture strain of the red alga Gracilariopsis chorda was suggested to be an undescribed taxon assigned to the family Flavobacteriaceae based on the 16S ribosomal RNA gene sequences. On the other hand, the results of morphological and phenotypic classification suggest that the predominant bacteria may belong to the family Flavobacteriaceae. Both results of morphological and phenotypic classification and genetic assay were similar. Algal culture experiment with medium containing indole-3-acetic acid (IAA) was carried out, because some Flavobacteriaceae bacteria are known to produce an auxin, IAA. A simple rapid technique in normal phase partition chromatography for monosaccharide and disaccharide analysis in edible seaweeds was developed. Algal components were measured by HPLC, GC-MS, and LC-MS. A bacterial product, IAA accelerated algal growth rate, and changed algal amino acid and saccharide compositions.
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