Isolation and characterization of bacteria playing a key role in nitrogen and sulfur cycle

• Project/Area Number: 25340086
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Modeling and technologies for environmental conservation and remediation
• Research Institution: Kumamoto University
• Principal Investigator: Morimura Shigeru 熊本大学, 自然科学研究科, 准教授 (20230146)
• Co-Investigator(Kenkyū-buntansha): KAWAGOSHI Yasunori 熊本大学, 大学院自然科学研究科, 教授 (00291211)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 微生物相 / 干潟 / 窒素循環 / 硫黄循環 / クローン解析 / 定量PCR / 機能性遺伝子 / 硫黄酸化細菌 / 脱窒菌 / 元素循環 / 干潟底泥環境 / 単離 / 生物機能利用 / 干潟環境

Outline of Final Research Achievements

The same uncultured clone was detected in clone analysis targeting nirS and aprA gene in tidal flat sediment sampled from Midorikawa, Shirakawa, and Yatsushiro sea. To clarify the characteristics of the uncultured clone, enrichment culture and isolation were corried out from the tidal flat sediments. Especially in sulfur oxidizing bacteria, which are important for sulfur cycle in tidal flat, both of heterotrophic and autotrophic bacteria were obtained. Therefore, not single group like sulfate reducing bacteria but multiple number of various sulfur oxidizing bacteria construct the oxidation reaction cycle.

Research Products

• [Journal Article] Isolation and characterization of heterotrophic thiosulfate-oxidizing bacteria and their possible role in the Midorikawa Tidal Flat, Ariake Sea, Japan (2015)
  • Author(s): Irfan Mustafa, Hiroto Ohta, Takuro Niidome, Shigeru Morimura
  • Journal Title: Journal of Ecology and the Natural Environment Volume: 7 Issue: 4 Pages: 106-112
  • DOI: 10.5897/jene2015.0504
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Isolation of thiosulfate oxidizers and their hypothetical roles in sulfur cycle of Midorikawa tidal flat (2014)
  • Author(s): Irfan Mustafa, Hiroto Ohta, Takuro Niidome, Shigeru Morimura
  • Organizer: GelK 5th International Symposium
  • Place of Presentation: Kumamoto, Japan
  • Year and Date: 2014-12-01
• [Presentation] Sulfur-oxidizing heterotrophic gammaproteobacteria at conserved tidal flats at the Ariake Sea (2014)
  • Author(s): Irfan Mustafa, Hiroto Ohta, Takuro Niidome, Shigeru Morimura
  • Organizer: The 2nd Seoul International Conference on Biological Engineering and Natural Science
  • Place of Presentation: Seoul, South Korea
  • Year and Date: 2014-08-29 – 2014-08-31
• [Presentation] Analysis of bacterial community for sulfur cycle in conserved tidal flats in Ariake sea
  • Author(s): Ifran Mustafa, Takao Yoshihara, Shigeru Morimura, Hiroto Ohta, Takuro Niidome, Tatsuya Masuda, Kiyoshi Takikawa
  • Organizer: 第65回日本生物工学会大会
  • Place of Presentation: 広島国際会議場
• [Presentation] 干潟底泥における硫黄酸化細菌群の解析
  • Author(s): 村中俊一朗、葭原孝雄、Tran Thanh Liem、太田広人、新留琢郎、増田龍哉、滝川 清、森村 茂
  • Organizer: 第20回日本生物工学会九州支部大会
  • Place of Presentation: 佐賀大学
• [Presentation] 干潟の硫黄循環に関与する微生物の定量的解析に関する検討
  • Author(s): 山口久美子、Irfan Mustafa、太田広人、新留琢郎、増田龍哉、滝川 清、森村 茂
  • Organizer: 第20回日本生物工学会九州支部大会
  • Place of Presentation: 佐賀大学