Isolation and characterization of bacteria playing a key role in nitrogen and sulfur cycle
| Project/Area Number |
25340086
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Modeling and technologies for environmental conservation and remediation
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAWAGOSHI Yasunori 熊本大学, 大学院自然科学研究科, 教授 (00291211)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 微生物相 / 干潟 / 窒素循環 / 硫黄循環 / クローン解析 / 定量PCR / 機能性遺伝子 / 硫黄酸化細菌 / 脱窒菌 / 元素循環 / 干潟底泥環境 / 単離 / 生物機能利用 / 干潟環境 |
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| Outline of Final Research Achievements |
The same uncultured clone was detected in clone analysis targeting nirS and aprA gene in tidal flat sediment sampled from Midorikawa, Shirakawa, and Yatsushiro sea. To clarify the characteristics of the uncultured clone, enrichment culture and isolation were corried out from the tidal flat sediments. Especially in sulfur oxidizing bacteria, which are important for sulfur cycle in tidal flat, both of heterotrophic and autotrophic bacteria were obtained. Therefore, not single group like sulfate reducing bacteria but multiple number of various sulfur oxidizing bacteria construct the oxidation reaction cycle.
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Report
(4 results)
Research Products
(6 results)
