Development of a high-throughput system for separation, cultivation and metabolism analysis on uncultured microorganisms
| Project/Area Number |
25340087
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Modeling and technologies for environmental conservation and remediation
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| Research Institution | Nigata University of Phermacy and Applied Life Sciences |
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Principal Investigator |
Shigematsu Toru 新潟薬科大学, 応用生命科学部, 教授 (10315286)
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| Co-Investigator(Kenkyū-buntansha) |
IGUCHI Akinori 新潟薬科大学, 応用生命科学部, 助教 (60599786)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | メタン発酵 / 嫌気性微生物 / 微生物共生系 / 酢酸酸化細菌 / メタン生成古細菌 / 難培養性微生物 / ハイスループット / 分離培養 / 微生物分離培養技術 / 酢酸利用微生物 / マイクロプレート |
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| Outline of Final Research Achievements |
To develop a high-throughput microbial separation and cultivation method for unculturable microorganisms, a liquid cultivation method using 96-well microplates was applied for anaerobic microorganisms in a methane fermentation process. Aceticlastic methanogens affiliated with the genera Methanosaeta and Methanosarcina, as well as hydrogenotrophic methanogen affiliated with the genus Methanolinea, were separately cultivated on a medium with acetate as the sole carbon source. Acetate-oxidizing bacteria, which could form syntrophic associations with hydrogenotrophic methnaogens, could not be identified. This study would provide a novel culture-dependent method for analysis on the taxonomic diversity and physiological functions of anaerobic acetate-oxidizing syntrophs with hydrogenotrophic methanogens, which play important roles for acetate conversion to methane in methane fermentation processes.
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Report
(4 results)
Research Products
(1 results)
