| Project/Area Number |
25350115
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Eating habits
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| Research Institution | Nakamura Gakuen College |
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Principal Investigator |
OHTA CHIHO 中村学園大学, 栄養科学部, 講師 (80271435)
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| Co-Investigator(Kenkyū-buntansha) |
KOGA Nobuyuki 中村学園大学, 栄養科学部, 教授 (80136514)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAMOTO Kenta 中村学園大学, 栄養科学部, 助手 (50709582)
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| Research Collaborator |
OGATA Hitomi
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | ポリメトキシフラボン類 / カラマンダリン果皮 / 熱安定性 / 抗酸化活性 / 生体内動態 / nobiletin / heptamethoxyflavone / ポリメトキシフラボノイド類 / natsudaidain / ラット / in vivo代謝 / 脱メチル化 / HPLC / heptamethosyflavone / 肝ミクロゾーム / in vitro代謝 |
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| Outline of Final Research Achievements |
Naturally occurring polymethoxyflavones found in Kara mandarin (Citrus reticulate Blanco), NBL and HeptaMF, showed high stability for heat treatment at 100 degrees Celsius. More than 99.8% of both NBL and HeptaMF dosed was absorbed from the gastrointestinal tract in rats. The demethylation was a main pathway in the metabolism of both compounds by CYP3A and CYP1A enzymes. There was a species difference in metabolic pattern of both compounds. Anti-oxidative activity of extracts from the peel of Kara mandarin was increased by heat treatment at 100 degrees Celsius. Metabolites of HeptaMF had higher anti-oxidative activity than HeptaMF itself. NBL was a weak inhibitor of transporters such as P-glycoprotein and MRP2 in Caco-2 cells.
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