Study of Novel receptors recognizing un-Methylated DNA
| Project/Area Number |
25350143
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Eating habits
|
|---|
| Research Institution | Showa University |
|---|
Principal Investigator |
Sawa Chika 昭和大学, 医学部, 講師 (80422541)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
Shioda Seiji 星薬科大学, 先端生命科学, 教授 (80102375)
|
|---|
| Research Collaborator |
Yofu Sachiko
Kiriyama Keisuke
|
|---|
| Project Period (FY) |
2013-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Keywords | 細胞外核酸 / マクロファージ / 酸化ストレス / 細胞増殖障害 / 免疫賦活 / 核酸 / 受容体 / マイクロアレイ / 自然免疫 |
|---|
| Outline of Final Research Achievements |
In this study, I clarified the following.
(1)Extracellular nucleic acid acts directly on cancer cells and has cell growth suppression. (2) dATP acts on macrophage-like cell line (PMA induced U937 cell line) and induces the expression of various factors such as IL-8 and THBS-1 (Thrombospondin 1). Furthermore, for the purpose of elucidating the cytotoxic mechanism by dATP, we tried to analyze the possibility of promoting oxidative stress. As a result, (3) induction of superoxide generation was confirmed only in the addition of dATP in the macrophage-like cell line.
I propose the possibility that nucleic acids released from necrotic cells and neutrophils act on nearby macrophages and release oxidative stress, thus causing damage to inflammatory cells.
|
Report
(5 results)
Research Products
(16 results)
-
-
-
-
-
-
-
-
-
-
[Presentation] Nucleoprotein affect on Cell-Cycle progression in human cancer cells2015
Author(s)
Sachiko Yofu, Keisuke Kiriyama, Junko Shibato, Randeep Rakwal, Chika Sawa, Tomomi Saito, Satomi Kishi, Masahito Sugi, Koji Usumi, Masaji Matsunaga, Seiji Shioda
Organizer
第120回日本解剖学会総会・全国学術集会
Place of Presentation
神戸
Year and Date
2015-03-21 – 2015-03-23
Related Report
-
-
-
-
-
-
