| Project/Area Number |
25350143
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Eating habits
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| Research Institution | Showa University |
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Principal Investigator |
Sawa Chika 昭和大学, 医学部, 講師 (80422541)
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| Co-Investigator(Renkei-kenkyūsha) |
Shioda Seiji 星薬科大学, 先端生命科学, 教授 (80102375)
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| Research Collaborator |
Yofu Sachiko
Kiriyama Keisuke
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 細胞外核酸 / マクロファージ / 酸化ストレス / 細胞増殖障害 / 免疫賦活 / 核酸 / 受容体 / マイクロアレイ / 自然免疫 |
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| Outline of Final Research Achievements |
In this study, I clarified the following.
(1)Extracellular nucleic acid acts directly on cancer cells and has cell growth suppression. (2) dATP acts on macrophage-like cell line (PMA induced U937 cell line) and induces the expression of various factors such as IL-8 and THBS-1 (Thrombospondin 1). Furthermore, for the purpose of elucidating the cytotoxic mechanism by dATP, we tried to analyze the possibility of promoting oxidative stress. As a result, (3) induction of superoxide generation was confirmed only in the addition of dATP in the macrophage-like cell line.
I propose the possibility that nucleic acids released from necrotic cells and neutrophils act on nearby macrophages and release oxidative stress, thus causing damage to inflammatory cells.
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