The study on the mechanism of time- and location-specific differentiation in the epithelium of small intestine
| Project/Area Number |
25350167
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Eating habits
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
BABA Ryoko 産業医科大学, 医学部, 講師 (90271436)
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| Co-Investigator(Kenkyū-buntansha) |
MORIMOTO Hiroyuki 産業医科大学, 医学部, 教授 (30335806)
FUJITA Mamoru 中村学園大学, 栄養科学部, 教授 (60037471)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 小腸 / 吸収上皮細胞 / 乳飲期 / トランスサイトーシス / エンドサイトーシス / オルガノイド / 空腸 / 回腸 / 幹細胞 |
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| Outline of Final Research Achievements |
We have reported that the absorptive cells of small intestine differ by age and its location. However, variation of the cells was not well known. To elucidate the differentiation mechanism of the cells, we analyzed the epithelium of small intestine by using electron microscope, DNA microarray and organoid methods. During suckling period, the absorptive cells of upper part of small intestine had well-developed apical transcytic membrane system. The cells of lower part had well-developed apical endocytic membrane system including giant lysosome. On the other hand, in adult, the absorptive cells of small intestine had neither apical transcytic nor endocytic membrane system. In the microarray analysis, the expression level of time- and location-specific structure and function related genes tended to be higher. In the organoid analysis, the absorptive cells derived from isolated crypt had similar time- and location-depended structure. Further investigation is needed to confirm our findings.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] High glucose concentration-induced expression of pentraxin-3 in a rat model of continuous peritoneal dialysis.2016
Author(s)
Ishimatsu N, Miyamoto T, Ueno H, Hasegawa E, Kuma A, Fujimoto Y, Bando K, Nakamata J, Furuno Y, Serino R, Baba R, Morimoto H, Doi Y, Tamura M, Otsuji Y.
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Journal Title
Histol Histopathol.
Volume: 31
Related Report
Peer Reviewed
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[Journal Article] Diamine oxidase as a marker of intestinal mucosal injury and the effect of soluble dietary fiber on gastrointestinal tract toxicity after intravenous 5-fluorouracil treatment in rats.2014
Author(s)
Fukudome I, Kobayashi M, Dabanaka K, Maeda H, Okamoto K, Okabayashi T, Baba R, Kumagai N, Oba K, Fujita M, Hanazaki K.
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Journal Title
Med Mol Morphol.
Volume: 47
Issue: 2
Pages: 100-7
DOI
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Peer Reviewed
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[Presentation] Morphological analysis of small intestinal organoids2015
Author(s)
Takahashi H, Baba R, Ishimatsu N, Morimoto H, Fujita M.
Organizer
The 120th Annual Meeting of The Japanese Society of Anatomists and the 92nd Annual Meeting of The Physiological Society of Japan
Place of Presentation
神戸国際会議場・展示場（兵庫県・神戸市）
Year and Date
2015-03-21 – 2015-03-23
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