| Project/Area Number |
25350180
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Eating habits
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| Research Institution | Osaka City Institute of Public Health and Environmental Sciences |
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Principal Investigator |
Nakamura Hiromi 大阪市立環境科学研究所, 調査研究課 微生物保健グループ, 研究主任 (00332445)
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| Co-Investigator(Kenkyū-buntansha) |
井口 純 宮崎大学, 農学部, 准教授 (00437948)
西川 禎一 大阪市立大学, 大学院生活科学研究科, 教授 (60183539)
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| Co-Investigator(Renkei-kenkyūsha) |
ABE NIICHIROU 大阪市立環境科学研究所, 調査研究課微生物保健グループ, 研究副主幹 (10321936)
WADA TAKAYUKI 長崎大学, 熱帯医学研究所, 助教 (70332450)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | リステリア / PFGE / MLST / 遺伝子型別 / 自由生活性アメーバ / 食品製造施設 / 食品 / アイスクリーム / アメーバ / 定着 / 施設定着 / バイオフィルム / 菌体外多糖 |
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| Outline of Final Research Achievements |
One hundred and forty-nine of Listeria monocytogenes (LM) strains isolated from the food and the food-processing plants were classified by genetic analysis (PFGE and MLST). We detected LM and free-living amoebae (FLA) in 81 swab samples obtained from the three lightly-pickled vegetables processing plants. As the result of this, we specified some genetic types of LM which were consistently detected from several foods processed by different manufacturers. Three swab samples from two different plants detected both LM and FLA, of which two samples were from packaging machines.
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