Application of Hydrophobic Micropatterns to Immunosensor with Fluid Control Mechanism for Improving Sensitivity

• Project/Area Number: 25350517
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Shinshu University (2015); Iwate University (2013-2014)
• Principal Investigator: YAMAGUCHI Masaki 信州大学, 学術研究院繊維学系, 教授 (50272638)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 計測工学 / 生物・生体工学 / 分子認識 / 流体工学 / ストレス

Outline of Final Research Achievements

The purpose of this study is to clarify the effectiveness of periodic structure applied to this type of fluid control mechanism to improve the sensitivity of biosensors. We evaluated the functionality of centrifugal fluid valve with micrometer sized periodic structure in the micro-flow channel. A disposable compact disc-based chip with centrifugal fluid valve was designed for this evaluation. In this case, parallel pillars showed higher hydrophobicity than cross-shaped pillars. The transferred volume ratio increased by 20% compared to that without a micro-periodic structure when a protein solution with a similar concentration to that in saliva and plasma was used. It was shown that the static hydrophobicity dominates the switching characteristics in the proposed centrifugal fluid valve. Our study suggests that applying periodic structure to fluid control mechanisms is an effective means of realizing hydrophobic surfaces.

Research Products

• [Journal Article] 微細周期構造の付与で検出特性を改善した気泡センサ (2016)
  • Author(s): 山口 昌樹，平岡 仁
  • Journal Title: 電気学会論文誌 E Volume: Vol.136, No.4 Pages: 1186-1189
  • NAID: 130005141449
  • Peer Reviewed
• [Journal Article] Disposable Collection Kit for Rapid and Reliable Collection of Saliva (2015)
  • Author(s): Masaki Yamaguchi, Yuki Tezuka, Kazunori Takeda, and Vivek Shetty
  • Journal Title: American Journal of Human Biology Volume: Vol.27 Issue: 5 Pages: 720-723
  • DOI: 10.1002/ajhb.22696
  • NAID: 120007136178
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Application of hydrophobic micropatterns to centrifugal fluid valve in flow channel (2015)
  • Author(s): Tomoki Shimakura and Masaki Yamaguchi
  • Journal Title: Journal of Adhesion Science and Technology Volume: Vol.29 Issue: 23 Pages: 2565-2575
  • DOI: 10.1080/01694243.2015.1073826
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 唾液バイオマーカーはストレスを発信する (2015)
  • Author(s): 山口 昌樹
  • Journal Title: ストレス科学 Volume: Vol.29, No.4 Pages: 355-365
• [Journal Article] Injection-molded Plastic Plate with Hydrophobic Surface by Nano-periodic Structure Applied in Uniaxial Direction (2015)
  • Author(s): Masaki Yamaguchi, Shinya Sasaki, Shojiro Suzuki, Yuki Nakayama
  • Journal Title: The American Journal of Human Biology Volume: Vol.29 Issue: 1 Pages: 24-35
  • DOI: 10.1080/01694243.2014.973158
  • Peer Reviewed / Open Access
• [Journal Article] Relationship between Salivary Cortisol and Depression in Adolescent Survivors of a Major Natural Disaster (2014)
  • Author(s): Takashi Yonekura, Kazunori Takeda, Vivek Shetty, Masaki Yamaguchi
  • Journal Title: The Journal of Physiological Sciences Volume: Vol.64 Issue: 4 Pages: 261-267
  • DOI: 10.1007/s12576-014-0315-x
  • NAID: 120007136668
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Automated-immunosensor with centrifugal fluid valves for salivary cortisol measurement (2014)
  • Author(s): Masaki Yamaguchi, Hiroki Katagata, Yuki Tezuka, Daisuke Niwa, Vivek Shetty
  • Journal Title: Sensing and BioSensing Research Volume: Vol.1 Pages: 15-20
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Proposal for a Power Supply Line-free Mass Sensor for Measuring Total Protein in Human Saliva (2014)
  • Author(s): Masaki Yamaguchi, and Yuta Kimura
  • Journal Title: Sensor Letters Volume: Vol.12 Issue: 6 Pages: 1186-1189
  • DOI: 10.1166/sl.2014.3165
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 30種の精油を用いた嗜好性の個人差に関する実態調査 (2013)
  • Author(s): 中山 友紀，長山 優，山口 昌樹
  • Journal Title: アロマテラピー学雑誌 Volume: Vol.13 Pages: 21-28
  • NAID: 40019668528
  • Peer Reviewed
• [Journal Article] 香辛料により引き起こされる生理反応の唾液バイオマーカーを用いた評価 (2013)
  • Author(s): 中野 敦行，長山 優，山口 昌樹
  • Journal Title: ライフサポート Volume: Vol.25 Pages: 63-67
  • NAID: 10031193009
  • Peer Reviewed
• [Journal Article] Stress evaluation in adult patients with atopic dermatitis using salivary cortisol. (2013)
  • Author(s): Mizawa M, Yamaguchi M, Ueda C, Makino T, Shimizu T.
  • Journal Title: Bio Med Res Int. Volume: 2013; 138027. Pages: 138027-138027
  • DOI: 10.1155/2013/138027
  • Peer Reviewed
• [Journal Article] Saliva Sampling Method affects Performance of a Salivary α-Amylase Biosensor (2013)
  • Author(s): Theodore F. Robles, Masaki Yamaguchi, Vivek Shetty
  • Journal Title: The American Journal of Human Biology Volume: Vol.25 Issue: 6 Pages: 719-724
  • DOI: 10.1002/ajhb.22438
  • Peer Reviewed
• [Journal Article] Photomask Patterning for Slope-Form Deep-Etching Using Deep-Reactive-Ion Etching and Gradation Exposure (2013)
  • Author(s): Masaki Yamaguchi and Yuki Nakayama
  • Journal Title: Sensors and Materials Volume: Vol.26 Pages: 31-37
  • Peer Reviewed
• [Presentation] 液滴の動的挙動の数値解析方法の提案 (2016)
  • Author(s): 坂田 哲大，横井 研介，山口 昌樹
  • Organizer: 第25回ライフサポート学会フロンティア講演会
  • Place of Presentation: 東京工科大学 (大田区)
  • Year and Date: 2016-03-08
• [Presentation] フェムト秒レーザーを用いたナノ微細周期構造の作成とそのデバイス応用 (2016)
  • Author(s): 山口 昌樹
  • Organizer: 第84回レーザ加工学会講演会
  • Place of Presentation: 三菱電機㈱ (名古屋市)
  • Year and Date: 2016-01-20
  • Invited
• [Presentation] 唾液バイオマーカーは心身ストレスを発信する (2015)
  • Author(s): 山口 昌樹
  • Organizer: 日本動物細胞工学会2015
  • Place of Presentation: 東北大学 (仙台市)
  • Year and Date: 2015-07-09
  • Invited
• [Presentation] Non-invasive Biosensors for Stress Monitoring (2014)
  • Author(s): Masaki Yamaguchi
  • Organizer: UK-Japan Workshop: Biosensing Technology for Innovative Healthcare
  • Place of Presentation: 英国大使館 (千代田区)
  • Year and Date: 2014-12-01 – 2014-12-02
  • Invited
• [Presentation] A Disc-shaped Power Supply Line-free Mass Sensor for Measuring Biomarker (2014)
  • Author(s): Masaki Yamaguchi, DongSu You, Yuki Nakayama
  • Organizer: The Fifth International Conference on Sensor Device Technologies and Application
  • Place of Presentation: Lisbon (Portugal)
  • Year and Date: 2014-11-17 – 2014-11-19
• [Presentation] 唾液バイオマーカーはストレスを発信する (2014)
  • Author(s): 山口 昌樹
  • Organizer: 日本ストレス学会学術総会第30回記念大会
  • Place of Presentation: 日本大学 (世田谷区)
  • Year and Date: 2014-11-07
  • Invited
• [Presentation] Analytical Properties of a Centrifugal Immunosensor for Salivary Cortisol (2014)
  • Author(s): Masaki Yamaguchi, Hiroki Katagata, Daisuke Niwa, Vivek Shetty
  • Organizer: 24th Anniversary World Congress on Biosensors
  • Place of Presentation: Melbourne (Australia)
  • Year and Date: 2014-05-27 – 2014-05-30
• [Presentation] Portable Salivary Cortisol Immunosensor with a Centrifugal Fluid Valve
  • Author(s): Masaki Yamaguchi, Shohei Sasaki, Vivek Shetty
  • Organizer: 3rd International Conference on Bio-Sensing Technology
  • Place of Presentation: Sitges (Spain)
• [Presentation] Proposal for a power supply line-free mass sensor using separate excitation for saliva samples
  • Author(s): M. Yamaguchi and Y. Kimura
  • Organizer: International Symposium on Olfaction and Electronic Nose
  • Place of Presentation: Daegu (Korea)
• [Presentation] Disposable and Quantitative Saliva Collection Kit for Point-Of-Care Diagnostics
  • Author(s): M. Yamaguchi, Y. Tezuka
  • Organizer: 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: 大阪国際会議場 (大阪市)
• [Presentation] Threshold Levels for Wettability in Nano- and Micro-meter Periodic Structures
  • Author(s): Yamaguchi M, Sasaki S
  • Organizer: 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: 大阪国際会議場 (大阪市)
• [Presentation] 唾液コルチゾール測定器の開発とビッグデータの活用
  • Author(s): 山口 昌樹
  • Organizer: 第66回日本自律神経学会総会
  • Place of Presentation: 愛知県産業労働センター (名古屋市)
  • Invited
• [Presentation] ストレス検査の現状と展望 ―唾液でわかる運動の効用―
  • Author(s): 山口 昌樹
  • Organizer: 公益社団法人日本フィットネス協会 ACSM CECセミナー
  • Place of Presentation: 国立オリンピック記念青少年総合センター (渋谷区)
  • Invited
• [Book] 非侵襲的検体検査の最前線，第II編第4章1節唾液検査のストレス測定への展開 (2015)
  • Author(s): 山口 昌樹，他
  • Total Pages: 261
  • Publisher: シーエムシー出版
• [Book] 次世代ヘルスケア機器の新製品開発，第3章第7節唾液バイオマーカーによる診断技術とストレスの可視化 (2014)
  • Author(s): 山口 昌樹，他
  • Total Pages: 684
  • Publisher: 技術情報協会
• [Book] ウェットプロセスによる精密薄膜コーティング技術，第3章第6節[9]フィルム・プラスチックへの微細周期構造の形成と濡れ性の制御 (2014)
  • Author(s): 山口 昌樹，他
  • Total Pages: 561
  • Publisher: 技術情報協会
• [Book] スマート・ヒューマンセンシング，第1編 第8章 健康回復支援のための唾液ストレスセンサ (2014)
  • Author(s): 三林 浩二 監修，山口 昌樹
  • Total Pages: 265
  • Publisher: シーエムシー出版
• [Book] 皮膚の測定評価法バイブル，第1部第7章第3節 紫外線による皮膚のダメージ度を皮膚マーカーで評価したい (2013)
  • Author(s): 菅原 隆 編，山口 昌樹，清水 忠道
  • Total Pages: 740
  • Publisher: 技術情報協会
• [Book] Biochemical Sensors - Mimicking Gustatory and Olfactory Senses, Chapter 28: Evaluating the Psychobiologic Effects of Air Conditioner through Biomarkers (2013)
  • Author(s): Kiyoshi Toko Eds., Masaki Yamaguchi and Hajime Nishimiya
  • Total Pages: 560
  • Publisher: Pan Stanford Publishing Pte Ltd.
• [Book] パーソナルヘルスケア，第2編 第6章 第3節 唾液ホルモンから慢性ストレスを可視化するポータブル機器の開発 (2013)
  • Author(s): エヌ・ティー・エス 編，山口 昌樹
  • Total Pages: 359
  • Publisher: エヌ・ティー・エス
• [Remarks] 信州大学 山口研究室
  • URL: http://fiber.shinshu-u.ac.jp/yamaguchi/TOP.html
• [Remarks] 岩手大学 山口・佐々木研究室
  • URL: http://www.mech.iwate-u.ac.jp/~yamaguchi/
• [Patent(Industrial Property Rights)] Resonant type mass sensor (2014)
  • Inventor(s): 山口 昌樹
  • Industrial Property Rights Holder: 信州大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-08-21
  • Acquisition Date: 2015-05-05
  • Overseas
• [Patent(Industrial Property Rights)] 三次元造形装置及びワイヤ貫入機構ユニット (2014)
  • Inventor(s): 山口 昌樹
  • Industrial Property Rights Holder: 岩手大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-139042
  • Filing Date: 2014-07-04
• [Patent(Industrial Property Rights)] 三次元造形装置及びワイヤ張設機構ユニット (2014)
  • Inventor(s): 山口 昌樹
  • Industrial Property Rights Holder: 岩手大学
  • Industrial Property Rights Type: 実用新案
  • Filing Date: 2014-07-04
  • Acquisition Date: 2014-08-27
• [Patent(Industrial Property Rights)] 心身状態関連情報提供装置，心身状態関連情報提供方法およびプログラム (2014)
  • Inventor(s): 山口 昌樹
  • Industrial Property Rights Holder: 岩手大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-029643
  • Filing Date: 2014-02-19
• [Patent(Industrial Property Rights)] マイクロ流路装置 (2014)
  • Inventor(s): 千葉 豪，山口 昌樹
  • Industrial Property Rights Holder: 大日本印刷，岩手大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-049331
  • Filing Date: 2014-03-12