Creation of multi-theranostics nanoparticle for multi-strategic therpnosis

• Project/Area Number: 25350530
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Yamaguchi University (2016); The University of Tokushima (2013-2015)
• Principal Investigator: NAKAMURA Michihiro 山口大学, 医学(系)研究科(研究院), 教授 (10314858)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 有機シリカ粒子 / セラノスティックス / 光線力学療法 / 磁場温熱療法 / 光線温熱療法 / 診断と治療の一体化 / マルチモーダルイメージング / ナノ医療 / セラノスティック / 光線力学的治療 / 磁場温熱治療

Outline of Final Research Achievements

We developed novel type of silica nanoparticles, organosilica nanoparticles that are both structurally and functionally different from typical ones and have advantages for multifunctionalization. We have prepared multifunctionalized organosilica nanoparticles for multimodal imaging. In this study we prepared theranostic organosilica nanoparticles enable to combine diagnostics and therapy. We could theranostic organosilica nanoparticles with various pharmacological effects. Multi-theranostic organosilica nanoparticles showed cytotoxic activity on single cell imaging. Improved accumulation of multi-theranostics organosilica nanoparticles in tumor tissue were confirmed using near-infrared in vivo imaging. Furthermore multi-theranostics organosilica nanoparticles revealed therapeutic effects using tumor-bearing mice.

Research Products

• [Int'l Joint Research] UCLA(米国)
• [Int'l Joint Research] City of Hope(米国)
• [Int'l Joint Research] University of California, Los Angeles(米国)
• [Journal Article] Relaxometric Property of Organosilica Nanoparticles Internally Functionalized with Iron Oxide and Fluorescent Dye for Multimodal Imaging (2017)
  • Author(s): Michihiro Nakamura, Koichiro Hayashi, Hitoshi Kubo, Takafumi Kanadani, Masafumi Harada, Toshinobu Yogo
  • Journal Title: Journal of Colloid and Interface Science Volume: 492 Pages: 127-135
  • DOI: 10.1016/j.jcis.2017.01.004
  • Peer Reviewed
• [Journal Article] One-Pot Synthesis of Dual Stimulus-Responsive Degradable Hollow Hybrid Nanoparticles for Image-guided Trimodal Therapy (2016)
  • Author(s): Koichiro Hayashi, Takuma Maruhashi, Wataru Sakamoto, Toshinobu Yogo
  • Journal Title: Advanced Functional Materials Volume: 26 Issue: 47 Pages: 8613-8622
  • DOI: 10.1002/adfm.201603394
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Surface Treatment of Zinc Oxide Nanoparticles by Silica Coating and Evaluation of Their Dispersibility and Photoluminescent Properties (2015)
  • Author(s): Hideki Hashimoto, Ryosuke Tanino,Michihiro Nakamura, Yasuhisa Fujita
  • Journal Title: e-Journal of Surface Science and Nanotechnology Volume: 13 Issue: 0 Pages: 451-454
  • DOI: 10.1380/ejssnt.2015.451
  • NAID: 130005110967
  • ISSN: 1348-0391
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of polyethylene glycol-resistant macrophages on stealth imaging in vitro using fluorescent organosilica nanoparticles (2015)
  • Author(s): Nakamura M, Hayashi K, Nakano M, Kanadani T, Miyamoto K, Kori T, Horikawa K.
  • Journal Title: ACS Nano. Volume: 9 Issue: 2 Pages: 1058-1071
  • DOI: 10.1021/nn502319r
  • Peer Reviewed
• [Journal Article] Photostable Iodinated Silica/Porphyrin Hybrid Nanoparticles with Heavy-Atom Effect for Wide-Field Photodynamic/Photothermal Therapy Using Single Light Source (2014)
  • Author(s): Koichiro Hayashi, Michihiro Nakamura, Hirokazu Miki, Shuji Ozaki, Masahiro Abe, Toshio Matsumoto, Toshinari Kori, and Kazunori Ishimura
  • Journal Title: Advanced Functional Materials Volume: 24 Issue: 4 Pages: 503-513
  • DOI: 10.1002/adfm.201301771
  • Peer Reviewed
• [Journal Article] Magnetically responsive smart nanoparticles for cancer treatment with a combination of magnetic hyperthermia and remote-control drug release (2014)
  • Author(s): Hayashi K, Nakamura M, Miki H, Ozaki S, Abe M, Matsumoto T, Sakamoto W, Yogo T, Ishimura K
  • Journal Title: Theranostics Volume: 4 Issue: 8 Pages: 834-844
  • DOI: 10.7150/thno.9199
  • Peer Reviewed / Open Access
• [Presentation] Photo-biomedical Application of Multifunctional Organosilica Nanoparticles (2016)
  • Author(s): Michihiro Nakamura
  • Organizer: 10th Anniversary International Symposium on Nanomedicine (ISNM2016) (Keynote Speech of Special Session )
  • Place of Presentation: Auditorium of National Institute of Advanced Industrial Science and Technology (茨城県・つくば市)
  • Year and Date: 2016-11-23
  • Int'l Joint Research / Invited
• [Presentation] 多機能有機シリカナノ粒子の医学生物学的応用 (2016)
  • Author(s): 中村教泰
  • Organizer: 日本解剖学会第７１回中国・四国支部学術集会
  • Place of Presentation: 岡山大学 鹿田キャンパス (岡山県・岡山市)
  • Year and Date: 2016-10-22
• [Presentation] シリカナノ粒子の高機能セラノスティック (2016)
  • Author(s): 中村教泰
  • Organizer: 第32回日本DDS学会学術集会(シンポジウム：次世代DDSのためのマテリアル戦略)
  • Place of Presentation: グランシップ（静岡県コンベンションアーツセンター）(静岡県・静岡市)
  • Year and Date: 2016-06-20
  • Invited
• [Presentation] Multifunctionalized Organosilica Nanoparticles for Single Cell Imaging, Analysis and Characterization (2016)
  • Author(s): Michihiro Nakamura
  • Organizer: The 4th Japan-China Symposium on Nanomedicine
  • Place of Presentation: 北九州国際会議場 (福岡県・北九州市)
  • Year and Date: 2016-05-12
  • Int'l Joint Research / Invited
• [Presentation] 蛍光ナノ粒子を用いたマクロファージ取り込みのタイムラプスイメージング. (2016)
  • Author(s): 中村教泰、木戸玲子、鶴尾吉宏
  • Organizer: 第121回日本解剖学会総会全国学術集会
  • Place of Presentation: ビッグパレットふくしま(福島県郡山市)
  • Year and Date: 2016-03-28
• [Presentation] In Vivo Macrophage Imaging using Near-infrared Fluorescent Organosilica Nanoparticles. (2015)
  • Author(s): Michihiro Nakamura
  • Organizer: 9th Internal Symposium on Nanomedicine.
  • Place of Presentation: Mie University (Tsu, Mie)
  • Year and Date: 2015-12-10
  • Int'l Joint Research / Invited
• [Presentation] Organosilica Nanoparticles for Biomedical Applications. (2015)
  • Author(s): Michihiro Nakamura
  • Organizer: International Symposium on EcoTopia Science 2015 (ISETS'15)
  • Place of Presentation: Nagoya University, Nagoya, Aichi, Japan
  • Year and Date: 2015-11-27
  • Int'l Joint Research / Invited
• [Presentation] 蛍光ナノ粒子を用いたマクロファージ取り込みのタイムラプスイメージング. (2015)
  • Author(s): 中村教泰、木戸玲子、鶴尾吉宏
  • Organizer: 日本解剖学会第７０回中国・四国支部学術集会
  • Place of Presentation: 愛媛大学城北キャンパス南加記念ホール(愛媛県松山市)
  • Year and Date: 2015-10-24
• [Presentation] Effective impairment of myeloma progenitors by hyperthermia: augmentation with bortezomib and Pim inhibition in combination (2015)
  • Author(s): H. Miki, T. Harada, S. Nakamura, H. Tenshin, R. Amachi, K. Watanabe, D. Hanson, J. Teramachi, H. Fujino, K. Sogabe, M. Takahashi, T. Maruhashi, S. Fujii, K. Kagawa, M. Nakamura, T. Matsumoto, M. Abe.
  • Organizer: 15th International Myeloma Workshop
  • Place of Presentation: Rome, Italy
  • Year and Date: 2015-09-23
  • Int'l Joint Research
• [Presentation] 中性子増感作用を有する多機能性BNCT剤の創薬研究 (2015)
  • Author(s): 宇都義浩、多田竜、山田久嗣、中村教泰、影治照善、増田開、中田栄司、森井孝、増永慎一郎.
  • Organizer: 第１回徳島ナノメディシン・シンポジウム
  • Place of Presentation: 徳島大学藤井節郎記念ホール(徳島県徳島市)
  • Year and Date: 2015-07-29
• [Presentation] 有機シリカ粒子技術とBNCTセラノスティックスへの展開 (2015)
  • Author(s): 中村 教泰, 鶴尾 吉宏, 宇都 義浩, 影治 照喜, 増田開
  • Organizer: 第１回徳島ナノメディシン・シンポジウム
  • Place of Presentation: 徳島大学藤井節郎記念ホール(徳島県徳島市)
  • Year and Date: 2015-07-29
• [Presentation] Multifunctionalized Organosilica Nanoparticles for Single Cell Imaging, Analysis and Characterization. (2015)
  • Author(s): Michihiro Nakamura.
  • Organizer: 3rd China-Japan Symposium on Nanomedicine
  • Place of Presentation: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China
  • Year and Date: 2015-06-19
  • Int'l Joint Research / Invited
• [Presentation] 熱療法は薬剤耐性骨髄腫前駆細胞を効率的に障害する. (2015)
  • Author(s): 三木浩和、原田武志、藤井志朗 、天知良太、天真寛文、渡邉佳一郎、Hanson Derek、寺町順平 、八木ひかる、 曽我部公子 、髙橋真美子、丸橋朋子 、宇髙憲吾 、中村信元 、賀川久美子 、中村教泰、松本俊夫、安倍正博.
  • Organizer: 第40回 日本骨髄腫学会学術集会
  • Place of Presentation: くまもと森都心プラザ(熊本県熊本市)
  • Year and Date: 2015-05-16
• [Presentation] Syntheses, characterization, and biomedical applications of fluorescent organosilica nanoparticles (2015)
  • Author(s): Michihiro Nakumura
  • Organizer: 249th ACS National Meeting & Exposition
  • Place of Presentation: Colorado Convention Center (Denver, USA)
  • Year and Date: 2015-03-22 – 2015-03-26
• [Presentation] Single cell imaging and analysis for macrophage uptake of nanoparticles using fluorescent organosilica nanoparticles. (2015)
  • Author(s): Mutsuki Nakano, Michihiro Nakamura, Koichiro Hayashi, Takafumi Kanadani, Kazue Miyamoto
  • Organizer: 249th ACS National Meeting & Exposition
  • Place of Presentation: Colorado Convention Center (Denver, USA)
  • Year and Date: 2015-03-22 – 2015-03-26
• [Presentation] Time-lapse imaging of endosome acidification using pH-responsive fluorescent organosilica nanoparticles. (2015)
  • Author(s): Marika Iseki, Michihiro Nakamura.
  • Organizer: 第120回日本解剖学会総会・全国学術集会
  • Place of Presentation: 神戸国際会議場 (兵庫県神戸市)
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Development of organosilica nanoparticle for photodynamic therapy (PDT) and single cell analysis of PDT effect. (2015)
  • Author(s): Fumitaka.Koga, Michihiro.Nakumura, Katuhiro.Atagi, Koichiro.Hayashi.
  • Organizer: 第120回日本解剖学会総会・全国学術集会
  • Place of Presentation: 神戸国際会議場 (兵庫県神戸市)
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Preparation of near-infrared organosilica nanoparticle and its application to in vivo fluorescent imaging. (2015)
  • Author(s): Katsuhiro Atagi, Michihiro Nakamura, Takuya Murakami.
  • Organizer: 第120回日本解剖学会総会・全国学術集会
  • Place of Presentation: 神戸国際会議場 (兵庫県神戸市)
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Multimodal imaging using multifunctional nanoparticles. (2015)
  • Author(s): Michihiro Nakumura
  • Organizer: NCAP (Nanocancer Asia Pacific Network) at APAN39
  • Place of Presentation: 福岡国際会議場 (福岡県福岡市)
  • Year and Date: 2015-03-03
• [Presentation] Single Cell Imaging and Analysis using Fluorescent Organosilica Nanoparticles. (2014)
  • Author(s): Michihiro Nakumura
  • Organizer: 8th International Symposium on Nanomedicine (ISNM2014)
  • Place of Presentation: 愛媛大学 (愛媛県松山市)
  • Year and Date: 2014-12-03 – 2014-12-05
  • Invited
• [Presentation] Single Cell Imaging and Analysis using Fluorescent Organosilica Nanoparticles. (2014)
  • Author(s): Michihiro Nakumura
  • Organizer: The Second Japan-China Symposium on Nanomedicine
  • Place of Presentation: 広島大学 (広島県広島市)
  • Year and Date: 2014-05-16 – 2014-05-17
  • Invited
• [Presentation] 有機シリカ・マルチ蛍光ナノプローブによるSeamless蛍光イメーシング (2013)
  • Author(s): 中村教泰、林幸壱朗、三木浩和、尾崎修治、安倍正博、松本俊夫、石村和敬
  • Organizer: 第7回日本分子イメージング学会総会・学術集会
  • Place of Presentation: 横浜赤レンガ倉庫1号館(神奈川県横浜市)
• [Presentation] Biomedical Applications of Organosilica Nanoparticles (2013)
  • Author(s): Michihiro Nakamura
  • Organizer: THE CHINA-JAPAN SYMPOSIUM ON NANOMEDICINE
  • Place of Presentation: 東南大学(南京, 中国)
  • Invited
• [Presentation] Organosilica Nanoparticles for Multimodal imaging (2013)
  • Author(s): M. Nakamura, K. Hayashi, H. Kubo, M. Harada, K. Izumi, K. Ishimura
  • Organizer: 7th International Symposium on Nanomedicine (ISNM2013)
  • Place of Presentation: 九州工業大学 (福岡県北九州市)
  • Invited
• [Remarks] 有機シリカ粒子技術 Organosilica Particle Technology
  • URL: http://organosilica.com
• [Remarks] 山口大学医学部医学科器官解剖学 Organ Anatomy and Nanomedicine
  • URL: http://ds.cc.yamaguchi-u.ac.jp/~org-anat/