| Project/Area Number |
25350533
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kumamoto University |
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Principal Investigator |
Morotomi Keiko 熊本大学, パルスパワー科学研究所, 特別研究員 (80639435)
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| Co-Investigator(Renkei-kenkyūsha) |
AKIYAMA Hidenori 熊本大学, パルスパワー科学研究所, 教授 (50126827)
YANO Ken-ichi 熊本大学, パルスパワー科学研究所, 教授 (70311230)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ナノ秒電気パルス / 癌治療 / 細胞死 / ネクローシス / ポリADPリボース / カルシウム / スフェロイド / アポトーシス |
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| Outline of Final Research Achievements |
This study was aimed to establish a new method using nanosecond electric pulses for cancer therapy. First, electrical conditions for efficient cell death induction were optimized. Next, cell death mechanisms were analyzed, and the induction of necrotic cell death and its associating poly ADP-ribose formation on cellular proteins were demonstrated. The presence of calcium augmented the cytotoxic effects of nanosecond electric pulses, and its absence conferred resistance to nanosecond electric pulses. A new electrode using a pair of tungsten carbide needles was manufactured and was examined for cell death induction. Finally, the cytotoxic effects of nanosecond electric pulses were confirmed by using spheroids, which are considered to be a tumor model in vitro. Taken together, these results contribute the development of a novel cancer therapy using nanosecond electric pulses.
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