| Project/Area Number |
25350537
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Osaka City University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Mitsuhiro 大阪市立大学, 大学院医学研究科, 講師 (40309571)
UEMURA Takuya 大阪市立大学, 大学院医学研究科, 病院講師 (10597321)
TAKAMATSU Kiyohito 大阪市立大学, 大学院医学研究科, 客員准教授 (30295688)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | iPS細胞 / 人工神経 / 末梢神経 / 再生医療 / 線維芽細胞増殖因子 / ドラッグデリバリーシステム / 成長因子 / FGF |
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| Outline of Final Research Achievements |
For peripheral nerve repair, various modifications have been explored. Here, sciatic nerve gaps in mice were reconstructed in the following groups: nerve conduit alone (C group), nerve conduit coated with induced pluripotent stem cell (iPSc)-derived neurospheres (I group), nerve conduit coated with iPSc-derived neurospheres and basic fibroblast growth factor (bFGF)-incorporated gelatin microspheres (I+F group), and autograft (A group). The fastest functional recovery and the greatest axon regeneration occurred in the A group, followed in order by the I+F group, I group, and C group until 12 weeks after reconstruction. Thus, peripheral nerve regeneration using nerve conduits and functional recovery in mice were accelerated by a combination of iPSc-derived neurospheres and a bFGF drug delivery system. The combination of all three fundamental methodologies was essential and useful for peripheral nerve regenerative medicine.
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