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Explore the molecular mechanism of cellular differentiation induced by PAMPS gels.

Research Project

Project/Area Number 25350548
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionHokkaido University

Principal Investigator

Semba Shingo  北海道大学, 医学(系)研究科(研究院), 特任助教 (40466496)

Co-Investigator(Kenkyū-buntansha) YASUDA Kazunori  北海道大学, 医学研究科, 教授 (20166507)
津田 真寿美  北海道大学, 医学(系)研究科(研究院), 講師 (30431307)
Co-Investigator(Renkei-kenkyūsha) TSUDA Masumi  北海道大学, 医学研究科, 講師 (30431307)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords再生医工材料 / 生体材料 / ダブルネットワークゲル / ハイドロゲル / 軟骨再生 / 再生医工学材料
Outline of Final Research Achievements

It is known that PAMPS gel induces the spontaneous regeneration of damaged cartilage, however the detailed molecular mechanism remains unclear. In this study, we identified integrins as PAMPS-binding proteins and found that culturing on PAMPS gel drastically changed the activation of p38 MAP kinase in chondrocytic murine ATDC5 cell. It was also found that the gel enhanced the gene expression related with the extracellular matrix, and secreted extracellular matrix were accumulated in the gel, which enhanced the chondrogenic differentiation of ATDC5 cells cultured on PAMPS gel. These results suggest that PAMPS gel not only alters gene expression via intracellular signaling molecules, such as integrins and MAP kinases, but also functions as a molecular reservoir to accumulate the factors that promote cartilage differentiation.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (3 results)

All 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results) (of which Invited: 1 results)

  • [Journal Article] Synthetic PAMPS gel activates BMP/Smad signaling pathway in ATDC5 cells, which plays a significant role in the gel-induced chondrogenic differentiation.2016

    • Author(s)
      Goto K, Kimura T, Kitamura N, Semba S, Ohmiya Y, Aburatani S, Matsukura S, Tsuda M, Kurokawa T, Gong JP, Tanaka S, Yasuda K.
    • Journal Title

      J Biomed Mater Res A

      Volume: 未定 Issue: 3 Pages: 734-746

    • DOI

      10.1002/jbm.a.35615

    • NAID

      120005981343

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ハイドロゲルが誘導する軟骨細胞分化における細胞内情報伝達機構の解明2015

    • Author(s)
      仙葉愼吾
    • Organizer
      第5回北海道探索病理学研究シンポジウム
    • Place of Presentation
      ニューオータニイン札幌(北海道、札幌市)
    • Year and Date
      2015-10-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] PAMPSゲルが誘導する軟骨細胞分化における細胞内情報伝達機構の解明2014

    • Author(s)
      仙葉愼吾
    • Organizer
      ソフト&ウェットマテリアルが拓くライフイノベーション第1回研究成果報告シンポジウム
    • Place of Presentation
      北海道大学(北海道・札幌市)
    • Year and Date
      2014-06-26
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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