Elucidation of the mechanism of cell-to-cell variability in non-viral transfection
| Project/Area Number |
25350553
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Nagasaki University |
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Principal Investigator |
FUMOTO Shintaro 長崎大学, 医歯薬学総合研究科(薬学系), 准教授 (70380988)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 遺伝子・核酸工学材料 / 遺伝子導入機構 / エンドサイトーシス / 細胞間 / リポソーム / 高分子 / 細胞間差 |
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| Outline of Final Research Achievements |
The purpose of the present study was to elucidate the mechanism of cell-to-cell variability in non-viral transfection in HepG2 cells. In case of lipoplexes, there was no correlation between cellular association and transgene expression over the threshold of cellular association. Moreover, dispersion condition of the lipoplexes and oxidative stress were important pharmaceutical and cellular factors, respectively.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Secure and effective gene delivery system of plasmid DNA coated by polynucleotide.2015
Author(s)
Kodama Y, Ohkubo C, Kurosaki T, Egashira K, Sato K, Fumoto S, Nishida K, Higuchi N, Kitahara T, Nakamura T, Sasaki H.
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Journal Title
J Drug Target.
Volume: 23
Issue: 1
Pages: 43-51
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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