| Project/Area Number |
25350556
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kansai University |
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Principal Investigator |
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| Research Collaborator |
FUTAKI Yudai
YAMAMOTO Yuki
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 細胞集合体 / 周期性ペプチド / 線維芽細胞 / 肝細胞 / 軟骨細胞 / 間葉系幹細胞 / 旋回培養 / 分化誘導 / ALP活性 / 共培養システム / 幹細胞 / アルブミン産出 / グリコサミノグリカン |
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| Outline of Final Research Achievements |
In the presence of (Lys-Pro)n (n=10, 12) peptide in cell culture medium, cell aggregation was observed beginning on the 3 day of culture, and the cell aggregations formed maintained their shape over the 7-day. Cell aggregations are induced by periodic peptides to form aggregates of fibroblasts, hepatocytes, and human mesenchymal stem cells (hMSCs). We evaluated (Lys-Pro)n derivative periodic peptides, such as (Lys-Pro-Pro)8 and (Lys-Lys-Pro)8, with respect to the relationship between the structure of the peptide chain and the function of hepatocyte, and hMSC aggregates. The periodic peptides induced the formation of uniformly sized cell aggregates. Albumin production was comparable in cell aggregation and 2D monolayer cultures on day 1, increased dramatically on day 3. Periodic peptides induced cell aggregation exhibited a relatively higher albumin production with the 2D monolayer. A result of ALP activity of hMSC aggregation was higher than 2D monolayer cultures.
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