| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Outline of Final Research Achievements |
We have developed a poly(dimethylsiloxane) (PDMS)-based microfluidic device stable in low temperature and successfully cryopreserves cells inside. We inoculated the device with cells, cooled off to -80 degrees Celsius, and was evaluated a survival rate after thawing. The cells frozen and thawed within microchannels tended to rather die even they were transferred to conventional culture conditions after thawing.
In order to grasp a factor of this extinction, we then tried vacuum de-aeration of PDMS to reduce bubble formation in microchannels, and forming a dent structure at the middle of the microchannel to reduce flow velocity without letting a streamline exfoliate in the microchannel. It successfully increased the seeding density of the cells in the microchannels, and enabled quick removal of cryoprotective agent. By these improvements we have succeeded in cryopreservation of microfluidic devices in which cells were alive after freezing and thawing in the microchannels therein.
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