| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
This project aimed at development of a microfluidic chip (microchip) which enables facile and simultaneous detection of disease marker protein and nucleic acid by exploiting our original microchip technologies. First, we have succeeded in highly sensitive detection of a biomarker protein for prostate cancer by optimizing the antibody-supporting substrate. Second, we have developed a microchip for simultaneous detection of 3 different microRNAs. Finally, we tried measurements of 4 different microRNAs from a total RNA sample derived from human leucocytes. As a result, the measured values from the microchips well agreed with those from conventional quantitative polymerase chain reaction. These results indicate that the developed microchip is promising for application to real complex samples.
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